High-Efficiency Capture of Drug Resistant-Influenza Virus by Live Imaging of Sialidase Activity

  • PLoS One. 2016 May 27;11(5):e0156400. doi: 10.1371/journal.pone.0156400.
Yuuki Kurebayashi  1 ,  Tadanobu Takahashi  1 ,  Chihiro Tamoto  1 ,  Keiji Sahara  2 ,  Tadamune Otsubo  3 ,  Tatsuya Yokozawa  1 ,  Nona Shibahara  1  4 ,  Hirohisa Wada  4 ,  Akira Minami  1 ,  Kiyoshi Ikeda  3 ,  Takashi Suzuki  1
Affiliations
  • 1. Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka-shi, Shizuoka, Japan.
  • 2. Shizuoka Institute of Environment and Hygiene, Shizuoka-shi, Shizuoka, Japan.
  • 3. Department of Organic Chemistry, School of Pharmaceutical Sciences, Hiroshima International University, Kure-shi, Hiroshima, Japan.
  • 4. Shizuoka City Institute of Environmental Sciences and Public Health, Shizuoka-shi, Shizuoka, Japan.
PMID: 27232333 DOI: 10.1371/journal.pone.0156400
Abstract

Influenza A and B viruses possess a neuraminidase protein that shows sialidase activity. Influenza virus-specific neuraminidase inhibitors (NAIs) are commonly used for clinical treatment of influenza. However, some influenza A and B viruses that are resistant to NAIs have emerged in nature. NAI-resistant viruses have been monitored in public hygiene surveys and the mechanism underlying the resistance has been studied. Here, we describe a new assay for selective detection and isolation of an NAI-resistant virus in a speedy and easy manner by live fluorescence imaging of viral sialidase activity, which we previously developed, in order to achieve high-efficiency capture of an NAI-resistant virus. An NAI-resistant virus maintains sialidase activity even at a concentration of NAI that leads to complete deactivation of the virus. Infected cells and focuses (infected cell populations) of an oseltamivir-resistant virus were selectively visualized by live fluorescence sialidase imaging in the presence of oseltamivir, resulting in high-efficiency isolation of the resistant viruses. The use of a combination of Other NAIs (zanamivir, peramivir, and laninamivir) in the imaging showed that the oseltamivir-resistant virus isolated in 2008 was sensitive to zanamivir and laninamivir but resistant to peramivir. Fluorescence imaging in the presence of zanamivir also succeeded in selective live-cell visualization of cells that expressed zanamivir-resistant NA. Fluorescence imaging of NAI-resistant sialidase activity will be a powerful method for study of the NAI resistance mechanism, for public monitoring of NAI-resistant viruses, and for development of a new NAI that shows an effect on various NAI-resistant mutations.

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