Tetranortriterpenes and Limonoids from the Roots of Aphanamixis polystachya

  • Molecules. 2016 Sep 2;21(9):1167. doi: 10.3390/molecules21091167.
Ching-Jie Lin  1 ,  I-Wen Lo  2 ,  Yu-Chi Lin  3 ,  Shun-Ying Chen  4 ,  Ching-Te Chien  5 ,  Yao-Haur Kuo  6 ,  Tsong-Long Hwang  7  8 ,  Shorong-Shii Liou  9 ,  Ya-Ching Shen  10
Affiliations
  • 1. School of Pharmacy, College of Medicine, National Taiwan University, No. 33, Linsen S. Rd., Zhongzheng Dist., Taipei 10050, Taiwan. [email protected].
  • 2. School of Pharmacy, College of Medicine, National Taiwan University, No. 33, Linsen S. Rd., Zhongzheng Dist., Taipei 10050, Taiwan. [email protected].
  • 3. Department of Life Sciences, National Cheng Kung University, No. 1, University Road, Tainan 701, Taiwan. [email protected].
  • 4. Taiwan Forestry Research Institute, Council of Agriculture, Executive Yuan, No. 53, Nanhai Rd., Zhongzheng Dist., Taipei 10066, Taiwan. [email protected].
  • 5. Taiwan Forestry Research Institute, Council of Agriculture, Executive Yuan, No. 53, Nanhai Rd., Zhongzheng Dist., Taipei 10066, Taiwan. [email protected].
  • 6. National Research Institute of Chinese Medicine, Ministry of Health and Welfare, No. 155-1, Sec. 2, Linong St., Beitou District, Taipei 11221, Taiwan. [email protected].
  • 7. Graduate Institute of Natural Products, School of Traditional Medicine, College of Medicine, and Chinese Herbal Medicine Research Team, Healthy Aging Research Center, Chang Gung University, Taoyuan 33302, Taiwan. [email protected].
  • 8. Department of Cosmetic Science and Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, Taoyuan 33302, Taiwan. [email protected].
  • 9. Department of Pharmacy, Tajen University, No. 20, Weixin Rd., Yanpu Township, Pingtung 90741, Taiwan. [email protected].
  • 10. School of Pharmacy, College of Medicine, National Taiwan University, No. 33, Linsen S. Rd., Zhongzheng Dist., Taipei 10050, Taiwan. [email protected].
PMID: 27598114 DOI: 10.3390/molecules21091167
Abstract

Phytochemical investigation of the acetone extract from the roots of Aphanamixis polystachya resulted in isolation of four new tetranortriterpenes (1-4) in addition to one protolimonoid (methyl-1ξ,7R-diacetoxy-23R,25-dihydroxy-20S,24R-21,24-epoxy-3,4-seco-apotirucall-4(28),14(15)-diene-3-oate (5)), five known limonoids (rohituka 3 (6), rohituka 7 (7), nymania 1 (8), rubrin G (9), prieurianin (10)) and a steroid (2,3-dihydroxy-5-pregnan-16-one (11)). Their structures were determined by spectroscopic analyses, including 2D-NMR (COSY, HMQC, HMBC, and NOESY) and high-resolution electrospray ionization mass spectrometry (HRESIMS). Cytotoxic and anti-inflammatory activities of these compounds were evaluated. Compounds 4 and 5 showed significant inhibition against superoxide generation and Elastase release by human neutrophils in response to (formyl-l-methionyl-l-leucyl-l-phenylalanine/cytochalasin B) (FMLP/CB).

Keywords
Aphanamixis polystachya; anti-inflammatory activity; apotirucallane-type triterpenoid; cytotoxicity; limonoid.
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