Urolithin A Protects Porcine Oocytes from Artificially Induced Oxidative Stress Damage to Enhance Oocyte Maturation and Subsequent Embryo Development

  • Int J Mol Sci. 2025 Mar 26;26(7):3037. doi: 10.3390/ijms26073037.
Wen Shi  1 ,  Chaobin Qin  1 ,  Yanyan Yang  1 ,  Xiaofen Yang  1 ,  Yizhen Fang  1 ,  Bing Zhang  1 ,  Dong Wang  1 ,  Wanyou Feng  2 ,  Deshun Shi  1
Affiliations
  • 1. Guangxi Key Laboratory of Animal Breeding & Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
  • 2. School of Environmental and Life Sciences, Nanning Normal University, Nanning 530001, China.
PMID: 40243704 DOI: 10.3390/ijms26073037
Abstract

Both the livestock and biomedical fields require a large supply of high-quality mature oocytes. However, the in vitro maturation (IVM) process often leads to an accumulation of Reactive Oxygen Species (ROS), which can cause defects in oocyte meiosis and embryo development, ultimately compromising oocyte quality. Urolithin A (UA), known for its antioxidant properties, has not been thoroughly investigated for its potential to mitigate the negative effects of oxidative stress during the in vitro culturing of oocytes, and its underlying mechanism is not well understood. In this study, an in vitro oxidative stress model was established using porcine oocytes treated with H2O2, followed by exposure to varying concentrations of UA. The results revealed that 30 μM UA significantly improved both the quality of oocyte culture and the developmental potential of the resulting embryos. UA was found to enhance oocyte Autophagy, reduce oxidative stress-induced mitochondrial damage, and restore mitochondrial function. Additionally, it lowered ROS and DNA damage levels in the oocytes, maintained proper spindle/chromosome alignment and actin Cytoskeleton structure, promoted nuclear maturation, prevented abnormal cortical granule distribution, and supported oocyte cytoplasmic maturation. As a result, UA alleviated oxidative stress-induced defects in oocyte maturation and cumulus cell expansion, thereby improving the developmental potential and quality of parthenogenetic embryos. After supplementation with UA, pig parthenogenetic embryo pluripotency-related genes (Nanog and Sox2) and antiapoptotic genes (Bcl2) were upregulated, while proapoptotic genes (Bax) were downregulated. In conclusion, this study suggests that adding UA during IVM can effectively mitigate the adverse effects of oxidative stress on porcine oocytes, presenting a promising strategy for enhancing their developmental potential in vitro.

Keywords
autophagy; embryo development; meiotic maturation; mitochondrial function; oocyte quality; oxidative stress; urolithin A.
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