1. Metabolic Enzyme/Protease
    NF-κB
    Immunology/Inflammation
    Cell Cycle/DNA Damage
    Autophagy
    Apoptosis
  2. Drug Metabolite
    Reactive Oxygen Species
    DNA/RNA Synthesis
    Autophagy
    Apoptosis
  3. Urolithin A

Urolithin A 

Cat. No.: HY-100599 Purity: 98.05%
Handling Instructions

Urolithin A, a gut-microbial metabolite of ellagic acid, exerts anti-inflammatory, antiproliferative, and antioxidant properties. Urolithin A induces autophagy and apoptosis, suppresses cell cycle progression, and inhibits DNA synthesis.

For research use only. We do not sell to patients.

Urolithin A Chemical Structure

Urolithin A Chemical Structure

CAS No. : 1143-70-0

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Based on 1 publication(s) in Google Scholar

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Description

Urolithin A, a gut-microbial metabolite of ellagic acid, exerts anti-inflammatory, antiproliferative, and antioxidant properties. Urolithin A induces autophagy and apoptosis, suppresses cell cycle progression, and inhibits DNA synthesis[1][2].

In Vitro

Micromolar urolithin A concentrations induces both autophagy and apoptosis. Urolithin A suppresses cell cycle progression and inhibited DNA synthesis in human sw620 colorectal cancer cells[2].
Urolithin A shows antiproliferative effects and inhibits T24 and Caco-2 cell growth with IC50s of 43.9 and 49 μM, respectively[3].
Urolithin A exerts a dose- and time-dependent significant arrest at G2/M and S phases after treatments with 50 and 100 μM at 24 and 48 h compared to control cells. It induces cell apoptosis with 50 and 100 μM [4].
Urolithin A shows potent antiproliferative activity on HepG2 cells. When cell death is induced by Urolithin A, the expression of β-catenin, c-Myc and Cyclin D1 are decreased and TCF/LEF transcriptional activation is notably down-regulated. Urolithin A also increases protein expression of p53, p38-MAPK and caspase-3, but suppresses expression of NF-κB p65 and other inflammatory mediators[5].

In Vivo

The volume of paw edema is reduced at 1 h after oral administration of urolithin A. In addition, plasma in treated mice exhibited significant oxygen radical antioxidant capacity (ORAC) scores with high plasma levels of the unconjugated form at 1 h after oral administration of urolithin A[6].

Molecular Weight

228.20

Formula

C₁₃H₈O₄

CAS No.

1143-70-0

SMILES

O=C1C2=CC(O)=CC=C2C3=CC=C(O)C=C3O1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 30 mg/mL (131.46 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.3821 mL 21.9106 mL 43.8212 mL
5 mM 0.8764 mL 4.3821 mL 8.7642 mL
10 mM 0.4382 mL 2.1911 mL 4.3821 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (10.96 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.5 mg/mL (10.96 mM); Suspended solution; Need ultrasonic

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (10.96 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[2]

Human colon cancer cells HT-29 are treated for 24 and 48 h at 100 and 50 μM of Urolithin A and Iso Urolithin A aglycones and their glucuronide conjugates. Cell viability and proliferation are measured using a TC10 automated cell counter with the addition of Trypan blue for viability determination. IC50 values are determined by MTT assay[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[4]

Mice: Paw edema is induced in the right hind paw of ICR mice by the subcutaneous injection of 1% λ-carrageenan in pysiological saline (50 μL). The inflammation level is quantified by the volume of paw edema. Urolithin A dissolved in 0.5% carboxymethylcellulose suspension is orally administered to the mice at 1 or 6 h before carrageenan injection. The anti-inflammatory effects of urolithin A on carrageenan-induced edema in mice are analyzed[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 98.05%

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Keywords:

Urolithin ADrug MetaboliteReactive Oxygen SpeciesDNA/RNA SynthesisAutophagyApoptosisInhibitorinhibitorinhibit

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