Selenomethionine activates MAPK signaling to activate epidermal keratinocyte migration, speeding skin wound healing

  • Biochem Biophys Res Commun. 2025 Sep 8:778:152398. doi: 10.1016/j.bbrc.2025.152398.
Yijing Zhou  1 ,  Chuanrui Yang  2 ,  Jieli Bai  1 ,  Jin Yang  3 ,  Lin Chen  4 ,  Qin Yu  5 ,  Xiaoyan Wen  6 ,  Yafei Yang  7
Affiliations
  • 1. Zunyi Medical University, Zunyi, Guizhou, China; Affiliated Hospital of Chengdu University, Chengdu, Sichuan, China.
  • 2. Affiliated Hospital of Chengdu University, Chengdu, Sichuan, China; Chengdu University, Chengdu, Sichuan, China.
  • 3. Zunyi Medical University, Zunyi, Guizhou, China; Affiliated Hospital of Chengdu University, Chengdu, Sichuan, China. Electronic address: [email protected].
  • 4. Zunyi Medical University, Zunyi, Guizhou, China; Affiliated Hospital of Chengdu University, Chengdu, Sichuan, China. Electronic address: [email protected].
  • 5. Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, China.
  • 6. Affiliated Hospital of Chengdu University, Chengdu, Sichuan, China.
  • 7. The Third Affiliated Hospital of Shenzhen University (Luohu Hospital Group), Shenzhen University, Shenzhen, China.
PMID: 40706245 DOI: 10.1016/j.bbrc.2025.152398
Abstract

The skin, the body's biggest organ, is prone to persistent wounds when wound healing is impeded. Exploring safe and effective wound-healing drugs holds significant clinical importance. This study examined how selenomethionine (Se-Met) affects keratinocyte proliferation and migration using cell proliferation and scratch assays; an ICR mouse skin wound model was established to evaluate healing capacity and stained; and related gene activity was examined using RT-qPCR and Western blot. The results showed that Se-Met promoted the migration of HaCaT cells, regulated the MAPK pathway and MMP-2 and MMP-9 protein expression, improved wound healing rates in mice, enhanced tissue morphology, and decreased inflammatory markers like TNF-α, IL-6, and IL-1β's mRNA levels.

Keywords
Keratinocyte; MAPK pathway; Selenomethionine; Wound healing.
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