(±)-Silybin (Synonyms: Silibinin (mixture of Silybin A and Silybin B))

Cat. No.: HY-139637 Purity: 99.40%: Data Sheet
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(±)-Silybin is the racemate of Silybin (HY-N0779A). Silybin induces apoptosis and exhibits hepatoprotective, antioxidant, anti-inflammatory, anti-cancer activity.

For research use only. We do not sell to patients.

(±)-Silybin Chemical Structure

CAS No. : 36804-17-8

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
250 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
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Other Forms of (±)-Silybin:

Silybin In-stock

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Biological Activity
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Description

(±)-Silybin is the racemate of Silybin (HY-N0779A). Silybin induces apoptosis and exhibits hepatoprotective, antioxidant, anti-inflammatory, anti-cancer activity^[1]^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
B16-4A5	IC₅₀	>100 μM Compound: 5	Toxicity in mouse B16-4A5 cells assessed as inhibition of cell proliferation in presence of 1 mM theophylline after 72 hrs by WST8 dye reduction assay Toxicity in mouse B16-4A5 cells assessed as inhibition of cell proliferation in presence of 1 mM theophylline after 72 hrs by WST8 dye reduction assay	[PMID: 20189399]
B16-4A5	IC₅₀	21 μM Compound: 5	Inhibition of theophylline-stimulated melanogenesis in mouse B16-4A5 cells after 72 hrs Inhibition of theophylline-stimulated melanogenesis in mouse B16-4A5 cells after 72 hrs	[PMID: 20189399]
HEK293	IC₅₀	2.7 μM Compound: Silybin A	Inhibition of OATP1B3 (unknown origin) expressed in HEK293 cells assessed as reduction of [3H]estradiol-17beta-glucuronide uptake after 3 mins by beta-counting Inhibition of OATP1B3 (unknown origin) expressed in HEK293 cells assessed as reduction of [3H]estradiol-17beta-glucuronide uptake after 3 mins by beta-counting	[PMID: 23401473]
HEK293	IC₅₀	9.7 μM Compound: Silybin A	Inhibition of OATP1B1 (unknown origin) expressed in HEK293 cells assessed as reduction of [3H]estradiol-17beta-glucuronide uptake after 3 mins by beta-counting Inhibition of OATP1B1 (unknown origin) expressed in HEK293 cells assessed as reduction of [3H]estradiol-17beta-glucuronide uptake after 3 mins by beta-counting	[PMID: 23401473]
Hepatocyte	IC₅₀	29.9 μM Compound: Silybin	Hepatoprotective activity against D-GaIN/TNFalpha-induced cell death in mouse hepatocyte at 100 uM relative to control Hepatoprotective activity against D-GaIN/TNFalpha-induced cell death in mouse hepatocyte at 100 uM relative to control	[PMID: 29353722]
Hepatocyte	IC₅₀	33.6 μM Compound: Silybin	Hepatoprotective activity against D-GaIN/TNFalpha-induced cell death in mouse hepatocyte at 100 uM Hepatoprotective activity against D-GaIN/TNFalpha-induced cell death in mouse hepatocyte at 100 uM	[PMID: 29353722]
Hepatocyte	IC₅₀	38.8 μM Compound: Silybin	Hepatoprotective activity against D-GaIN-induced toxicity in mouse hepatocyte relative to control Hepatoprotective activity against D-GaIN-induced toxicity in mouse hepatocyte relative to control	[PMID: 29353722]
HepG2	EC₅₀	75.7 μM Compound: Silybin	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by SRB assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by SRB assay	[PMID: 29353722]
Huh-7	IC₅₀	80 μM Compound: 1	Cytotoxicity against human Huh7.5.1 cells after 72 hrs Cytotoxicity against human Huh7.5.1 cells after 72 hrs	[PMID: 23673225]
Huh7.5.1	CC₅₀	80 μM Compound: 3	Cytotoxicity against human Huh7.5.1 cells after 72 hrs by ATPlite assay Cytotoxicity against human Huh7.5.1 cells after 72 hrs by ATPlite assay	[PMID: 30485098]
HUVEC	IC₅₀	62.33 μM Compound: 1a, (2R,3R,10R,11R)	Cytotoxicity against HUVEC assessed as cell viability after 16 hrs by MTT assay Cytotoxicity against HUVEC assessed as cell viability after 16 hrs by MTT assay	[PMID: 21928794]
HUVEC	IC₅₀	8.83 μM Compound: 1a, (2R,3R,10R,11R)	Antiproliferative activity against HUVEC assessed as inhibition of cell growth after 3 days by MTT assay Antiproliferative activity against HUVEC assessed as inhibition of cell growth after 3 days by MTT assay	[PMID: 21928794]
LoVo	IC₅₀	50 μM Compound: Silybin A; Silibinin	Cytotoxicity against human LoVo cells assessed as reduction in cell growth after 24 hrs by trypan blue exclusion assay Cytotoxicity against human LoVo cells assessed as reduction in cell growth after 24 hrs by trypan blue exclusion assay	[PMID: 27517806]
LX-2	IC₅₀	151.1 μM Compound: Silybin	Cytotoxicity against human LX2 cells assessed as reduction in cell viability by MTT assay Cytotoxicity against human LX2 cells assessed as reduction in cell viability by MTT assay	[PMID: 33775837]
LX-2	IC₅₀	164.4 μM Compound: Silybin	Inhibition of hyaluronic acid deposition in human LX2 cells Inhibition of hyaluronic acid deposition in human LX2 cells	[PMID: 33775837]
LX-2	IC₅₀	51.5 μM Compound: Silybin	Inhibition of collagen type 1 deposition in human LX2 cells Inhibition of collagen type 1 deposition in human LX2 cells	[PMID: 33775837]
LX-2	IC₅₀	83.7 μM Compound: Silybin	Inhibition of laminin deposition in human LX2 cells Inhibition of laminin deposition in human LX2 cells	[PMID: 33775837]
MCF7	IC₅₀	222.8 μM Compound: 1	Cytotoxicity in human MCF7 cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay Cytotoxicity in human MCF7 cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay	[PMID: 28411546]
MDCK-II	IC₅₀	4.5 μM Compound: Silybin A	Inhibition of OATP2B1 (unknown origin) expressed in MDCK2 cells assessed as reduction of [3H]estrone-3-sulfate uptake after 3 mins by beta-counting Inhibition of OATP2B1 (unknown origin) expressed in MDCK2 cells assessed as reduction of [3H]estrone-3-sulfate uptake after 3 mins by beta-counting	[PMID: 23401473]
T47D	IC₅₀	110 μM Compound: Silybin A; Silibinin	Cytotoxicity against human T47D cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against human T47D cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 27517806]
T47D	IC₅₀	12 μM Compound: Silybin A; Silibinin	Downregulation of telomerase expression in human T47D cells after 72 hrs in presence of curcumin by SYBR green-dye based RT-PCR analysis Downregulation of telomerase expression in human T47D cells after 72 hrs in presence of curcumin by SYBR green-dye based RT-PCR analysis	[PMID: 27517806]
T47D	IC₅₀	20 μM Compound: Silybin A; Silibinin	Cytotoxicity against human T47D cells assessed as reduction in cell viability after 24 hrs in presence of curcumin by MTT assay Cytotoxicity against human T47D cells assessed as reduction in cell viability after 24 hrs in presence of curcumin by MTT assay	[PMID: 27517806]

Molecular Weight

482.44

Formula

C₂₅H₂₂O₁₀

CAS No.

36804-17-8

Appearance

Solid

Color

White to off-white

SMILES

OC[C@@H]1[C@@H](C2=CC(OC)=C(C=C2)O)OC3=CC([C@H]4OC5=CC(O)=CC(O)=C5C([C@@H]4O)=O)=CC=C3O1

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (103.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0728 mL	10.3640 mL	20.7280 mL
5 mM	0.4146 mL	2.0728 mL	4.1456 mL
10 mM	0.2073 mL	1.0364 mL	2.0728 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.40%

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Data Sheet (271 KB) SDS (393 KB)

COA (249 KB) HNMR (268 KB) RP-HPLC (351 KB)

Handling Instructions (2659 KB)

References

[1]. Silvia Zappavigna, et al. Silybin-Induced Apoptosis Occurs in Parallel to the Increase of Ceramides Synthesis and miRNAs Secretion in Human Hepatocarcinoma Cells. Int J Mol Sci. 2019 May 3;20(9):2190. [Content Brief]

[2]. Runbin Sun, et al. Silybin ameliorates hepatic lipid accumulation and modulates global metabolism in an NAFLD mouse model. Biomed Pharmacother. 2020 Mar;123:109721. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0728 mL	10.3640 mL	20.7280 mL	51.8199 mL
	5 mM	0.4146 mL	2.0728 mL	4.1456 mL	10.3640 mL
	10 mM	0.2073 mL	1.0364 mL	2.0728 mL	5.1820 mL
	15 mM	0.1382 mL	0.6909 mL	1.3819 mL	3.4547 mL
	20 mM	0.1036 mL	0.5182 mL	1.0364 mL	2.5910 mL
	25 mM	0.0829 mL	0.4146 mL	0.8291 mL	2.0728 mL
	30 mM	0.0691 mL	0.3455 mL	0.6909 mL	1.7273 mL
	40 mM	0.0518 mL	0.2591 mL	0.5182 mL	1.2955 mL
	50 mM	0.0415 mL	0.2073 mL	0.4146 mL	1.0364 mL
	60 mM	0.0345 mL	0.1727 mL	0.3455 mL	0.8637 mL
	80 mM	0.0259 mL	0.1295 mL	0.2591 mL	0.6477 mL
	100 mM	0.0207 mL	0.1036 mL	0.2073 mL	0.5182 mL

(±)-Silybin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(±)-Silybin36804-17-8Silibinin (mixture of Silybin A and Silybin B)Apoptosismilkthistlehepatoprotectiveantioxidantanti-inflammatoryanti-cancerHepG2G1-phaseS-phaseAKTInhibitorinhibitorinhibit

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(±)-Silybin (Synonyms: Silibinin (mixture of Silybin A and Silybin B))

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Other Forms of (±)-Silybin:

(±)-Silybin Related Antibodies

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References

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Complete Stock Solution Preparation Table

(±)-Silybin Related Classifications

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