Cat. No.: HY-139637

(±)-Silybin

(Synonyms: Silibinin (mixture of Silybin A and Silybin B))

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(±)-Silybin is the racemate of Silybin (HY-N0779A). Silybin induces apoptosis and exhibits hepatoprotective, antioxidant, anti-inflammatory, anti-cancer activity.

For research use only. We do not sell to patients.

Purity: 99.40%
CAS No.: 36804-17-8
Formula: C₂₅H₂₂O₁₀
Molecular Weight:482.44

Storage:

4°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

(±)-Silybin Chemical Structure

CAS No.: 36804-17-8

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

(±)-Silybin is the racemate of Silybin (HY-N0779A). Silybin induces apoptosis and exhibits hepatoprotective, antioxidant, anti-inflammatory, anti-cancer activity^[1]^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
B16-4A5	IC₅₀	>100 μM Compound: 5	Toxicity in mouse B16-4A5 cells assessed as inhibition of cell proliferation in presence of 1 mM theophylline after 72 hrs by WST8 dye reduction assay Toxicity in mouse B16-4A5 cells assessed as inhibition of cell proliferation in presence of 1 mM theophylline after 72 hrs by WST8 dye reduction assay	[PMID: 20189399]
B16-4A5	IC₅₀	21 μM Compound: 5	Inhibition of theophylline-stimulated melanogenesis in mouse B16-4A5 cells after 72 hrs Inhibition of theophylline-stimulated melanogenesis in mouse B16-4A5 cells after 72 hrs	[PMID: 20189399]
HEK293	IC₅₀	2.7 μM Compound: Silybin A	Inhibition of OATP1B3 (unknown origin) expressed in HEK293 cells assessed as reduction of [3H]estradiol-17beta-glucuronide uptake after 3 mins by beta-counting Inhibition of OATP1B3 (unknown origin) expressed in HEK293 cells assessed as reduction of [3H]estradiol-17beta-glucuronide uptake after 3 mins by beta-counting	[PMID: 23401473]
HEK293	IC₅₀	9.7 μM Compound: Silybin A	Inhibition of OATP1B1 (unknown origin) expressed in HEK293 cells assessed as reduction of [3H]estradiol-17beta-glucuronide uptake after 3 mins by beta-counting Inhibition of OATP1B1 (unknown origin) expressed in HEK293 cells assessed as reduction of [3H]estradiol-17beta-glucuronide uptake after 3 mins by beta-counting	[PMID: 23401473]
Hepatocyte	IC₅₀	29.9 μM Compound: Silybin	Hepatoprotective activity against D-GaIN/TNFalpha-induced cell death in mouse hepatocyte at 100 uM relative to control Hepatoprotective activity against D-GaIN/TNFalpha-induced cell death in mouse hepatocyte at 100 uM relative to control	[PMID: 29353722]
Hepatocyte	IC₅₀	33.6 μM Compound: Silybin	Hepatoprotective activity against D-GaIN/TNFalpha-induced cell death in mouse hepatocyte at 100 uM Hepatoprotective activity against D-GaIN/TNFalpha-induced cell death in mouse hepatocyte at 100 uM	[PMID: 29353722]
Hepatocyte	IC₅₀	38.8 μM Compound: Silybin	Hepatoprotective activity against D-GaIN-induced toxicity in mouse hepatocyte relative to control Hepatoprotective activity against D-GaIN-induced toxicity in mouse hepatocyte relative to control	[PMID: 29353722]
HepG2	EC₅₀	75.7 μM Compound: Silybin	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by SRB assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by SRB assay	[PMID: 29353722]
Huh-7	IC₅₀	80 μM Compound: 1	Cytotoxicity against human Huh7.5.1 cells after 72 hrs Cytotoxicity against human Huh7.5.1 cells after 72 hrs	[PMID: 23673225]
Huh7.5.1	CC₅₀	80 μM Compound: 3	Cytotoxicity against human Huh7.5.1 cells after 72 hrs by ATPlite assay Cytotoxicity against human Huh7.5.1 cells after 72 hrs by ATPlite assay	[PMID: 30485098]
HUVEC	IC₅₀	62.33 μM Compound: 1a, (2R,3R,10R,11R)	Cytotoxicity against HUVEC assessed as cell viability after 16 hrs by MTT assay Cytotoxicity against HUVEC assessed as cell viability after 16 hrs by MTT assay	[PMID: 21928794]
HUVEC	IC₅₀	8.83 μM Compound: 1a, (2R,3R,10R,11R)	Antiproliferative activity against HUVEC assessed as inhibition of cell growth after 3 days by MTT assay Antiproliferative activity against HUVEC assessed as inhibition of cell growth after 3 days by MTT assay	[PMID: 21928794]
LoVo	IC₅₀	50 μM Compound: Silybin A; Silibinin	Cytotoxicity against human LoVo cells assessed as reduction in cell growth after 24 hrs by trypan blue exclusion assay Cytotoxicity against human LoVo cells assessed as reduction in cell growth after 24 hrs by trypan blue exclusion assay	[PMID: 27517806]
LX-2	IC₅₀	151.1 μM Compound: Silybin	Cytotoxicity against human LX2 cells assessed as reduction in cell viability by MTT assay Cytotoxicity against human LX2 cells assessed as reduction in cell viability by MTT assay	[PMID: 33775837]
LX-2	IC₅₀	164.4 μM Compound: Silybin	Inhibition of hyaluronic acid deposition in human LX2 cells Inhibition of hyaluronic acid deposition in human LX2 cells	[PMID: 33775837]
LX-2	IC₅₀	51.5 μM Compound: Silybin	Inhibition of collagen type 1 deposition in human LX2 cells Inhibition of collagen type 1 deposition in human LX2 cells	[PMID: 33775837]
LX-2	IC₅₀	83.7 μM Compound: Silybin	Inhibition of laminin deposition in human LX2 cells Inhibition of laminin deposition in human LX2 cells	[PMID: 33775837]
MCF7	IC₅₀	222.8 μM Compound: 1	Cytotoxicity in human MCF7 cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay Cytotoxicity in human MCF7 cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay	[PMID: 28411546]
MDCK-II	IC₅₀	4.5 μM Compound: Silybin A	Inhibition of OATP2B1 (unknown origin) expressed in MDCK2 cells assessed as reduction of [3H]estrone-3-sulfate uptake after 3 mins by beta-counting Inhibition of OATP2B1 (unknown origin) expressed in MDCK2 cells assessed as reduction of [3H]estrone-3-sulfate uptake after 3 mins by beta-counting	[PMID: 23401473]
T47D	IC₅₀	110 μM Compound: Silybin A; Silibinin	Cytotoxicity against human T47D cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against human T47D cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 27517806]
T47D	IC₅₀	12 μM Compound: Silybin A; Silibinin	Downregulation of telomerase expression in human T47D cells after 72 hrs in presence of curcumin by SYBR green-dye based RT-PCR analysis Downregulation of telomerase expression in human T47D cells after 72 hrs in presence of curcumin by SYBR green-dye based RT-PCR analysis	[PMID: 27517806]
T47D	IC₅₀	20 μM Compound: Silybin A; Silibinin	Cytotoxicity against human T47D cells assessed as reduction in cell viability after 24 hrs in presence of curcumin by MTT assay Cytotoxicity against human T47D cells assessed as reduction in cell viability after 24 hrs in presence of curcumin by MTT assay	[PMID: 27517806]

Chemical Information

CAS No. 36804-17-8
Appearance Solid
Molecular Weight 482.44
Formula C₂₅H₂₂O₁₀
Color White to off-white
SMILES

OC[C@@H]1[C@@H](C2=CC(OC)=C(C=C2)O)OC3=CC([C@H]4OC5=CC(O)=CC(O)=C5C([C@@H]4O)=O)=CC=C3O1
Synonyms

Silibinin (mixture of Silybin A and Silybin B)
Structure Classification
- Flavonoids
- Flavanonols
Initial Source
- Plants
- Compositae
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (103.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.0728 mL

10.3640 mL

20.7280 mL

5 mM

0.4146 mL

2.0728 mL

4.1456 mL

10 mM

0.2073 mL

1.0364 mL

2.0728 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

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Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Silvia Zappavigna, et al. Silybin-Induced Apoptosis Occurs in Parallel to the Increase of Ceramides Synthesis and miRNAs Secretion in Human Hepatocarcinoma Cells. Int J Mol Sci. 2019 May 3;20(9):2190. [Content Brief]

[2]. Runbin Sun, et al. Silybin ameliorates hepatic lipid accumulation and modulates global metabolism in an NAFLD mouse model. Biomed Pharmacother. 2020 Mar;123:109721. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.0728 mL	10.3640 mL	20.7280 mL	51.8199 mL
	5 mM	0.4146 mL	2.0728 mL	4.1456 mL	10.3640 mL
	10 mM	0.2073 mL	1.0364 mL	2.0728 mL	5.1820 mL
	15 mM	0.1382 mL	0.6909 mL	1.3819 mL	3.4547 mL
	20 mM	0.1036 mL	0.5182 mL	1.0364 mL	2.5910 mL
	25 mM	0.0829 mL	0.4146 mL	0.8291 mL	2.0728 mL
	30 mM	0.0691 mL	0.3455 mL	0.6909 mL	1.7273 mL
	40 mM	0.0518 mL	0.2591 mL	0.5182 mL	1.2955 mL
	50 mM	0.0415 mL	0.2073 mL	0.4146 mL	1.0364 mL
	60 mM	0.0345 mL	0.1727 mL	0.3455 mL	0.8637 mL
	80 mM	0.0259 mL	0.1295 mL	0.2591 mL	0.6477 mL
	100 mM	0.0207 mL	0.1036 mL	0.2073 mL	0.5182 mL

Keywords

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(±)-Silybin

Apoptosis Compound Library

Natural Product Library

Anti-Cancer Compound Library

Antioxidant Compound Library

Lipid Compound Library

Traditional Chinese Medicine Active Compound Library

Flavonoids Library

Anti-Cancer Natural Product Library

Plant-Sourced Natural Product Library

Cell Death Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Anti-Fibrosis Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Natural Product Library

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

(±)-Silybin

Apoptosis Compound Library

Natural Product Library

Anti-Cancer Compound Library

Antioxidant Compound Library

Lipid Compound Library

Traditional Chinese Medicine Active Compound Library

Flavonoids Library

Anti-Cancer Natural Product Library

Plant-Sourced Natural Product Library

Cell Death Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Anti-Fibrosis Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Natural Product Library

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

(±)-Silybin Related Classifications

HY-139637 Related Classifications