ASK1 Protein, Human (His)

ASK1 protein is a key serine/threonine kinase in the MAP kinase pathway, coordinating cellular responses to environmental changes and affecting cell fate, differentiation, and survival. ASK1 is a key player in apoptosis and promotes mitochondria-dependent caspase activation. ASK1 Protein, Human (His) is the recombinant human-derived ASK1 protein, expressed by E. coli , with C-6*His labeled tag. The total length of ASK1 Protein, Human (His) is 298 a.a., .

ASK1, a serine/threonine kinase, functions as a pivotal component in the MAP kinase signal transduction pathway, playing a crucial role in orchestrating cellular responses to environmental changes. This kinase is instrumental in determining cell fate, influencing processes such as differentiation and survival. Notably, ASK1 is a key player in the apoptosis signal transduction pathway, facilitating mitochondria-dependent caspase activation. Moreover, MAP3K5/ASK1 is indispensable for the innate immune response, contributing significantly to host defense against a diverse array of pathogens. It serves as a mediator of signal transduction in response to various stressors, including oxidative stress, and responds to receptor-mediated inflammatory signals, such as those initiated by tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Upon activation, ASK1 acts as an upstream activator for both the MKK/JNK and p38 MAPK signal transduction cascades by phosphorylating and activating MAP kinase kinases such as MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6, and MAP2K7/MKK7. These activated MAP2Ks, in turn, initiate the activation of p38 MAPKs and c-jun N-terminal kinases (JNKs), both of which exert control over the transcription factors of the activator protein-1 (AP-1) family.

Human

E. coli

C-6*His

Q99683 (M649-K946)

4217

Approximately 34.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.