CAPN1 Protein, Mouse (His)

CAPN1 protein, a calcium-regulated non-lysosomal thiol-protease, facilitates cytoskeletal remodeling and signal transduction. It selectively cleaves substrates involved in these processes, including CTBP1 at 'Asn-375', 'Gly-388', and 'His-410'. CAPN1 also activates caspase-7 (CASP7), highlighting its role in regulating cellular functions. CAPN1 Protein, Mouse (His) is the recombinant mouse-derived CAPN1 protein, expressed by E. coli , with N-6*His labeled tag.

CAPN1 is a calcium-regulated non-lysosomal thiol-protease that plays a crucial role in cytoskeletal remodeling and signal transduction. It selectively catalyzes limited proteolysis of substrates involved in these processes. Specifically, CAPN1 targets CTBP1 and cleaves it at 'Asn-375', 'Gly-388', and 'His-410'. Additionally, CAPN1 is responsible for the cleavage and activation of caspase-7 (CASP7), further emphasizing its importance in regulating cellular functions.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

E. coli

N-6*His

O35350 (M1-A713)

12333  [NCBI]

Full Length

MTEELITPVYCTGVSAQVQKKRDKELGLGRHENAIKYLGQDYETLRARCLQSGVLFQDEAFPPVSHSLGFKELGPHSSKTYGIKWKRPTELMSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNETILHRVVPYGQSFQDGYAGIFHFQLWQFGEWVDVVIDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGCTSEAFEDFTGGVTEWYDLQKAPSDLYQIILKALERGSLLGCSINISDIRDLEAITFKNLVRGHAYSVTGAKQVTYQGQRVNLIRMRNPWGEVEWKGPWSDSSYEWNKVDPYEREQLRVKMEDGEFWMSFRDFIREFTKLEICNLTPDALKSRTLRNWNTTFYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLEEVDDADDYDNRESGCSFLLALMQKHRRRERRFGRDMETIGFAVYQVPRELAGQPVHLKRDFFLANASRAQSEHFINLREVSNRIRLPPGEYIVVPSTFEPNKEGDFLLRFFSEKKAGTQELDDQIQANLPDEKVLSEEEIDDNFKTLFSKLAGDDMEISVKELQTILNRIISKHKDLRTNGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLTIFRKFDLDKSGSMSAYEMRMAIEAAGFKLNKKLHELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKLLDTDLDGVVTFDLFKWLQLTMFA

Approximately 84 kDa, based on SDS-PAGE under reducing conditions.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.