NT5C2 Protein, Human

NT5C2 Protein, Human is the recombinant human-derived NT5C2, expressed by E. coli , with tag Free labeled tag. ,

NT5C2 is a broad specificity cytosolic 5'-nucleotidase that primarily catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates. It also exhibits phosphotransferase activity, transferring phosphate groups from donor nucleoside monophosphates to acceptor nucleosides (with preference for inosine, deoxyinosine, and guanosine). The enzyme shows highest activity toward IMP and GMP, followed by dIMP, dGMP, and XMP; while it can catalyze phosphate transfer from pyrimidine monophosphates, the efficiency is lower. These activities enable NT5C2 to regulate cellular purine nucleoside/nucleotide pools. by similar: Its phosphotransferase activity is more pronounced for inosine, deoxyinosine, and guanosine, with relatively reduced efficiency for pyrimidine monophosphates.

Human

E. coli

Tag Free

P49902-1 (M1-E561)

22978

Full Length of Isoform-1

MSTSWSDRLQNAADMPANMDKHALKKYRREAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVGKVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSHRPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESPLATRNRTSVDFKDTDYKRHQLTRSISEIKPPNLFPLAPQEITHCHDEDDDEEEEEEEE

Approximately 60 kDa

≥ 80%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50mM Tris-HCl (pH 8.0), 1mM DTT, 200mM NaCl, 5% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.