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PTK9 Protein, Human

Cat. No.: HY-P77167
COA Handling Instructions

PTK9, an actin-binding protein, regulates motility and morphological processes by inhibiting actin polymerization and capping the barbed ends of filaments. It plays a crucial role in clathrin-mediated endocytosis and the distribution of endocytic organelles. PTK9 interacts with G-actin, capping protein (CP), and possibly TWF2 and phosphoinositides, particularly PI(4,5)P2, which down-regulates its activity. Additionally, it interacts with ACTG1. PTK9 Protein, Human is the recombinant human-derived PTK9 protein, expressed by E. coli , with tag free. The total length of PTK9 Protein, Human is 252 a.a., with molecular weight of ~33 kDa.

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Description

PTK9, an actin-binding protein, regulates motility and morphological processes by inhibiting actin polymerization and capping the barbed ends of filaments. It plays a crucial role in clathrin-mediated endocytosis and the distribution of endocytic organelles. PTK9 interacts with G-actin, capping protein (CP), and possibly TWF2 and phosphoinositides, particularly PI(4,5)P2, which down-regulates its activity. Additionally, it interacts with ACTG1. PTK9 Protein, Human is the recombinant human-derived PTK9 protein, expressed by E. coli , with tag free. The total length of PTK9 Protein, Human is 252 a.a., with molecular weight of ~33 kDa.

Background

PTK9, an actin-binding protein, actively participates in motile and morphological processes, exerting its influence on actin polymerization by likely sequestering G-actin. Through its capacity to cap the barbed ends of filaments, PTK9 plays a regulatory role in cellular motility. Furthermore, its involvement in clathrin-mediated endocytosis and the distribution of endocytic organelles suggests a multifaceted role in cellular dynamics. PTK9 interacts with G-actin, particularly the ADP-actin form, and with capping protein (CP), highlighting its intricate involvement in actin-related processes. Additionally, it may have interactions with TWF2 and phosphoinositides, particularly PI(4,5)P2, where binding to the latter results in down-regulation. Moreover, PTK9 has been observed to interact with ACTG1, further expanding its network of molecular associations.

Species

Human

Source

E. coli

Tag

Tag Free

Accession

Q12792-4 (M1-D252)

Gene ID
Molecular Construction
N-term
PTK9 (M1-D252)
Accession # Q12792-4
C-term
Synonyms
Twinfilin-1; Protein A6; Protein tyrosine kinase 9; TWF1
AA Sequence

MLYAATRATLKKEFGGGHIKDEVFGTVKEDVSLHGYKKYLLSQSSPAPLTAAEEELRQIKINEVQTDVGVDTKHQTLQGVAFPISREAFQALEKLNNRQLNYVQLEIDIKNEIIILANTTNTELKDLPKRIPKDSARYHFFLYKHSHEGDYLESIVFIYSMPGYTCSIRERMLYSSCKSRLLEIVERQLQMDVIRKIEIDNGDELTADFLYEEVHPKQHAHKQSFAKPKGPAGKRGIRRLIRGPAETEATTD

Molecular Weight

Approximately 33 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

PTK9 Protein, Human Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
PTK9 Protein, Human
Cat. No.:
HY-P77167
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