PTK9 Protein, Human
Based on 1 Customer Validation
PTK9, an actin-binding protein, regulates motility and morphological processes by inhibiting actin polymerization and capping the barbed ends of filaments. It plays a crucial role in clathrin-mediated endocytosis and the distribution of endocytic organelles. PTK9 interacts with G-actin, capping protein (CP), and possibly TWF2 and phosphoinositides, particularly PI(4,5)P2, which down-regulates its activity. Additionally, it interacts with ACTG1. PTK9 Protein, Human is the recombinant human-derived PTK9 protein, expressed by E. coli , with tag free.
- Species: Human
- Source: E. coli
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
PTK9, an actin-binding protein, regulates motility and morphological processes by inhibiting actin polymerization and capping the barbed ends of filaments. It plays a crucial role in clathrin-mediated endocytosis and the distribution of endocytic organelles. PTK9 interacts with G-actin, capping protein (CP), and possibly TWF2 and phosphoinositides, particularly PI(4,5)P2, which down-regulates its activity. Additionally, it interacts with ACTG1. PTK9 Protein, Human is the recombinant human-derived PTK9 protein, expressed by E. coli , with tag free.
PTK9, an actin-binding protein, actively participates in motile and morphological processes, exerting its influence on actin polymerization by likely sequestering G-actin. Through its capacity to cap the barbed ends of filaments, PTK9 plays a regulatory role in cellular motility. Furthermore, its involvement in clathrin-mediated endocytosis and the distribution of endocytic organelles suggests a multifaceted role in cellular dynamics. PTK9 interacts with G-actin, particularly the ADP-actin form, and with capping protein (CP), highlighting its intricate involvement in actin-related processes. Additionally, it may have interactions with TWF2 and phosphoinositides, particularly PI(4,5)P2, where binding to the latter results in down-regulation. Moreover, PTK9 has been observed to interact with ACTG1, further expanding its network of molecular associations.
Technical Parameters
-
Species Human
-
Source E. coli
-
Tag Tag Free
-
Accession
Q12792-4 (M1-D252)
-
Molecular Construction
-
N-term
-
PTK9 (M1-D252)
Accession # Q12792-4 -
C-term
-
-
Protein Length
Full Length of Isoform-4
-
Synonyms
TWF1; PTK9; Twinfilin-1; Protein A6; Protein tyrosine kinase 9; A6 Protein Tyrosine Kinase; A6; Twinfilin, Actin-Binding Protein, Homolog 1; Twinfilin, Actin-Binding Protein, Homolog 1 (Drosophila); PTK9 Protein Tyrosine Kinase 9; Twinfilin Actin Binding
-
AA Sequence
MLYAATRATLKKEFGGGHIKDEVFGTVKEDVSLHGYKKYLLSQSSPAPLTAAEEELRQIKINEVQTDVGVDTKHQTLQGVAFPISREAFQALEKLNNRQLNYVQLEIDIKNEIIILANTTNTELKDLPKRIPKDSARYHFFLYKHSHEGDYLESIVFIYSMPGYTCSIRERMLYSSCKSRLLEIVERQLQMDVIRKIEIDNGDELTADFLYEEVHPKQHAHKQSFAKPKGPAGKRGIRRLIRGPAETEATTD
-
Predicted Molecular Mass
29 kDa
-
Molecular Weight
Approximately 36 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (236 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
