TrpB Protein, E.coli (His)

The TrpB protein, especially its β subunit, plays a crucial role in the synthesis of L-tryptophan from indole and L-serine. This enzyme function involves converting precursor molecules into the essential amino acid L-tryptophan, thereby facilitating its biosynthetic pathway. TrpB Protein, E.coli (His) is the recombinant E. coli-derived TrpB protein, expressed by E. coli , with N-6*His labeled tag.

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  • Species: E.coli
  • Source: E. coli
  • Biological Activity
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

The TrpB protein, especially its β subunit, plays a crucial role in the synthesis of L-tryptophan from indole and L-serine. This enzyme function involves converting precursor molecules into the essential amino acid L-tryptophan, thereby facilitating its biosynthetic pathway. TrpB Protein, E.coli (His) is the recombinant E. coli-derived TrpB protein, expressed by E. coli , with N-6*His labeled tag.

Background

The TrpB protein, specifically its beta subunit, assumes a crucial role in the synthesis of L-tryptophan from indole and L-serine. This enzymatic function highlights the beta subunit's involvement in the conversion of precursor molecules into the essential amino acid L-tryptophan, contributing to the biosynthetic pathway of this important cellular component. The enzymatic activity of TrpB underscores its significance in cellular metabolism, particularly in the synthesis of tryptophan, a fundamental building block for protein synthesis and various biological processes.

Technical Parameters

  • Species E.coli
  • Source E. coli
  • Tag N-6*His
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • 6*His
    • TrpB (T2-I397)
      Accession # P0A879
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    Tryptophan synthase beta chain; trpB

  • AA Sequence

    TTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQFNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMGKTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVHSGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILEREGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRVGIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALEAFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILKARGEI

  • Predicted Molecular Mass

    43.8 kDa

  • Molecular Weight

    Approximately 38-48 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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