TrpB Protein, E.coli (His)
The TrpB protein, especially its β subunit, plays a crucial role in the synthesis of L-tryptophan from indole and L-serine. This enzyme function involves converting precursor molecules into the essential amino acid L-tryptophan, thereby facilitating its biosynthetic pathway. TrpB Protein, E.coli (His) is the recombinant E. coli-derived TrpB protein, expressed by E. coli , with N-6*His labeled tag.
- Species: E.coli
- Source: E. coli
Biological Activity
The TrpB protein, especially its β subunit, plays a crucial role in the synthesis of L-tryptophan from indole and L-serine. This enzyme function involves converting precursor molecules into the essential amino acid L-tryptophan, thereby facilitating its biosynthetic pathway. TrpB Protein, E.coli (His) is the recombinant E. coli-derived TrpB protein, expressed by E. coli , with N-6*His labeled tag.
The TrpB protein, specifically its beta subunit, assumes a crucial role in the synthesis of L-tryptophan from indole and L-serine. This enzymatic function highlights the beta subunit's involvement in the conversion of precursor molecules into the essential amino acid L-tryptophan, contributing to the biosynthetic pathway of this important cellular component. The enzymatic activity of TrpB underscores its significance in cellular metabolism, particularly in the synthesis of tryptophan, a fundamental building block for protein synthesis and various biological processes.
Technical Parameters
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Species E.coli
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Source E. coli
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Tag N-6*His
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Accession
P0A879 (T2-I397)
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Gene ID58388660 [NCBI]
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Molecular Construction
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N-term
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6*His
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TrpB (T2-I397)
Accession # P0A879 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
Tryptophan synthase beta chain; trpB
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AA Sequence
TTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQFNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMGKTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVHSGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILEREGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRVGIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALEAFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILKARGEI
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Predicted Molecular Mass
43.8 kDa
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Molecular Weight
Approximately 38-48 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)