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  2. Rose-β-D-Gal

Rose-β-D-Gal 

Cat. No.: HY-W127769
Handling Instructions

Rose-β-D-Gal is a flurescent dye, is also a β-galactosidase substrate. Rose-β-D-Gal creates a pink/magenta color after the reaction and has been used for detection of β-gal activity.

For research use only. We do not sell to patients.

Rose-β-D-Gal Chemical Structure

Rose-β-D-Gal Chemical Structure

CAS No. : 138182-21-5

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Description

Rose-β-D-Gal is a flurescent dye, is also a β-galactosidase substrate. Rose-β-D-Gal creates a pink/magenta color after the reaction and has been used for detection of β-gal activity[1][2].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Immunostaining of Tissues[1][2]:
1.Prepare 25 mg/mL Rose-β-D-Gal stock solution in Dimethyl Sulfoxide (DMSO) and store at -20 °C. It is vital to protect the stock solution from the light.
2.Prepare rinse buffer for Rose-β-D-Gal staining: 0.1 % Sodium Deoxycholate, 0.2 % IGEPAL CA-630, 2 mM MgCl2 in 0.1 M Na Phosphate buffer (pH 7.3). Prepare 1,000 mL of 0.5 M Na Phosphate buffer (pH 7.3) by mixing with 158 mL of 1 M NaH2PO4, 342 mL of 1 M Na 2HPO4, and 500 mL water.
3.Prepare substrate solution for Rose-β-D-Gal fresh, containing 1 mg/mL Rose-β-D-Gal, 5 mM Potassium Ferricyanide, and 5 mM Potassium Ferrocyanide in the rinse buffer.
4.Rehydrate frozen tissue sections in PBS: quickly wash with PBS twice, wash with rinse buffer for 10 min 3 time.
5.Expose to β-galactosidase substrate Rose-β-D-Gal: stain at 37 ℃ for as long as needed to see stain, up to an overnight time period, keep covered and in the dark during color development. Wash with PBS for 5 min twice.
6.Fix tissue in 4% PFA, unless on intend to continue with in situ hybridizations, and continue to step 4.
Note: Rose-β-D-Gal is not very stable in alcohols or organic solvents, so must use water-based counterstains such as Gill’s hematoxylin and cover slipping with aqueous mountant.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

329.73

Formula

C14H16ClNO6

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Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
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Product Name:
Rose-β-D-Gal
Cat. No.:
HY-W127769
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