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Patent Blue V

" in MedChemExpress (MCE) Product Catalog:

By Targets:	Fluorescent Dye (3) Reference Standards (1)
By Research Areas:	Cancer (1)

Inhibitors & Agonists

Fluorescent Dyes

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-126395

Patent Blue V calcium salt	Fluorescent Dye	Others
Patent Blue V calcium salt is a triarylmethane dye used for tissue staining and lymphatic tracing, mainly applied by topical injection or eye drops. Patent Blue V calcium salt has affinity for specific tissues (such as corneal endothelium, lymphatic system), and stains the target structure by adsorption or binding, assisting in precise operation during surgery. Patent Blue V calcium salt is mainly used in ophthalmic surgery (such as graft staining for Descemet's membrane endothelial keratoplasty) and lymphatic drainage localization for sentinel lymph node biopsy of tumors .

HY-126395R

Patent Blue V calcium salt (Standard)	Fluorescent Dye Reference Standards	Others
Patent Blue V (calcium salt) (Standard) is the analytical standard of Patent Blue V (calcium salt). This product is intended for research and analytical applications. Patent Blue V has been widely used in sentinel lymph node mapping. Patent Blue V is also a food coloring agent and an alternative dye for trypan blue (TB) in descemet membrane endothelial keratoplasty (DMEK) .

HY-D0293

Acid blue 1 free acid Xylene Blue free acid; C.I. Acid Blue 1 free acid	Fluorescent Dye	Cancer
Acid blue 1 free acid (Patent Blue V) is a novel biological dye that can be used as an intraocular dye for retinectomy. Retinectomy refers to the removal of the translucent inner limiting membrane (ILM). The application of appropriate dyes in vitreoretinal surgery can achieve the purpose of complete removal. Acid blue 1 free acid can be used to stain retinal premembranous structures. Spectral analysis shows that Acid blue 1 free acid has strong absorption below 450 nm and above 600 nm, showing a blue-green color. Acid blue 1 free acid is also used as a marker in lymphangiography for resection of neoplastic lymph nodes .

Cat. No.	Product Name	Type

HY-D0293

Acid blue 1 free acid Xylene Blue free acid; C.I. Acid Blue 1 free acid	Fluorescent Dyes
Acid blue 1 free acid (Patent Blue V) is a novel biological dye that can be used as an intraocular dye for retinectomy. Retinectomy refers to the removal of the translucent inner limiting membrane (ILM). The application of appropriate dyes in vitreoretinal surgery can achieve the purpose of complete removal. Acid blue 1 free acid can be used to stain retinal premembranous structures. Spectral analysis shows that Acid blue 1 free acid has strong absorption below 450 nm and above 600 nm, showing a blue-green color. Acid blue 1 free acid is also used as a marker in lymphangiography for resection of neoplastic lymph nodes .

Acid blue 1 free acid

Xylene Blue free acid; C.I. Acid Blue 1 free acid

Fluorescent Dyes

Acid blue 1 free acid (Patent Blue V) is a novel biological dye that can be used as an intraocular dye for retinectomy. Retinectomy refers to the removal of the translucent inner limiting membrane (ILM). The application of appropriate dyes in vitreoretinal surgery can achieve the purpose of complete removal. Acid blue 1 free acid can be used to stain retinal premembranous structures. Spectral analysis shows that Acid blue 1 free acid has strong absorption below 450 nm and above 600 nm, showing a blue-green color. Acid blue 1 free acid is also used as a marker in lymphangiography for resection of neoplastic lymph nodes .

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BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

MedchemExpress Validation 03

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred

MedchemExpress Validation 04

MedchemExpress Validation

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