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Targeted therapy! The Capetin Prize winning "Click Chemistry" can be used like this!Targeted therapy! The Nobel Prize winning "Click Chemistry" can be used like this!2025-02-06
14 Results for "
confocal microscope
" in MedChemExpress (MCE) Product Catalog:
Products (14)
14 Results for "confocal microscope" in MCE Product Catalog:
Cat. No.: HY-D3126
CAS No.: 2420449-92-7
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IOPBA is a fluorescent probe that can be used for the detection and bioimaging of trivalent iron ions Fe 3+ and fluoride ions F - in living cells. IOPBA emits blue fluorescence in its native state, but upon the introduction of Fe 3+, it forms a Fe 3+-IOPBA π-complex, where Fe 3+ ions coordinate with two phenyl groups of IOPBA, resulting in fluorescence quenching. IOPBA returns to its free state and recovers its original fluorescence after F - sequesters Fe 3+ to form the more stable [FeF6] 3- and dissociates the Fe 3+-IOPBA complex. The excitation wavelength of IOPBA is 342 nm, and its emission wavelength is 458 nm .
Cat. No.: HY-D3098
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Mito‑VR2 is a mitochondria-targeted molecular rotor-type viscosity fluorescent probe with high responsiveness to microenvironmental viscosity, which is suitable for synchronous imaging of mitochondrial viscosity during autophagy in living cells. Mito-VR2 achieves specific targeting of mitochondria through its hydrophobic cationic structure, and its fluorescence intensity responds only to microenvironmental viscosity. Mito-VR2 emits almost no fluorescence in low-viscosity media, but its fluorescence emission is significantly enhanced in high-viscosity environments .
Cat. No.: HY-D3117
CAS No.: 2146114-18-1
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MBCB is a two-photon Fluorescent probe for dual-detection of mitochondrial SO₂ derivatives and viscosity. For SO₂ derivatives detection, the probe utilizes a Michael addition mechanism: nucleophilic addition of SO₂ derivatives to the C=C bond between the carbazole skeleton and 3-methylbenzothiazolium moiety destroys the strong intramolecular charge transfer (ICT) system between these groups, while enhancing the weak ICT system between the benzothiazole group and carbazole framework; this causes the red emission at 600 nm to decrease and the blue emission at 434 nm to increase, creating a ratiometric response based on the I₄₃₄ₙₘ/I₆₀₀ₙₘ intensity ratio. For viscosity detection, in low-viscosity environments, steric hindrance creates a twisted ICT (TICT) system with weak fluorescence, while in high-viscosity environments, intramolecular rotation is blocked, the TICT state is disrupted, and the strong ICT system is recovered, leading to a strong red emission at 567 nm with negligible change to the short-wavelength emission at 415 nm, creating a ratiometric response based on the I₅₆₇ₙₘ/I₄₁₅ₙₘ intensity ratio that has a logarithmic linear relationship with viscosity. The probe has excitation/emission wavelengths of Ex/Em = 351/434, 600 nm for SO₂ derivatives detection and Ex/Em = 351/567 nm for viscosity detection, with two-photon excitation at 740 nm for bioimaging; it also exhibits good mitochondrial targeting ability with a Pearson's colocalization coefficient of 0.93 when paired with Mito-Tracker Green. The probe shows high sensitivity and selectivity for SO₂ derivatives, has low cell cytotoxicity, and can be applied to detect exogenous/endogenous HSO₃⁻ in living cells and in vivo, as well as visualize mitochondrial viscosity changes induced by nystatin[1].
Cat. No.: HY-D3367
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Th-TMR-S-SNAP is a fluorescent probe used for SNAP-tag labeling and wash-free live-cell imaging of intracellular SNAP-tag fusion proteins. The excitation wavelength of Th-TMR-S-SNAP is 561 nm, and its emission wavelength ranges from 570 to 620 nm .
Cat. No.: HY-D3119
CAS No.: 2246946-49-4
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MitoAIE1 is a Fluorescent probe for mitochondrial viscosity detection. MitoAIE1 contains a pyridinium structural unit, which endows it with mitochondria-targeting specificity. The detection mechanism of this probe is based on aggregation-induced emission (AIE): in low-viscosity media such as PBS buffer, intramolecular rotation leads to non-radiative energy dissipation, resulting in only weak fluorescence; however, in high-viscosity environments such as the mitochondrial matrix or the vicinity of the inner mitochondrial membrane, such intramolecular motion is restricted, thereby significantly enhancing its fluorescent signal; in addition, this probe is not interfered by changes in microenvironment polarity and pH. Its emission wavelength is 625 nm, with absorption peaks at 325 nm and 450 nm; when transferred from low-viscosity PBS to high-viscosity 99% glycerol, its fluorescence intensity at 625 nm can be increased by 38-fold. It can be used to monitor changes in mitochondrial viscosity during processes such as Stauroporine (HY-15141)-induced apoptosis and starvation-induced mitophagy in live cells, and has good biocompatibility .
Cat. No.: HY-D3090
CAS No.: 2757682-04-3
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NRLD is a lipid droplet-targeting solvatochromic fluorescent probe developed based on Nile Red (HY-D0718), which can target lipid droplets and sense the non-polar lipid microenvironment inside lipid droplets. NRLD exhibits superior lipid droplet-targeting selectivity compared to the parent Nile Red dye in HeLa cell imaging. NRLD achieves detection relying on the solvatochromic effect generated by excited-state charge transfer, and its emission wavelength shifts according to the local polarity changes of the microenvironment it locates in: the more compact and ordered the lipid packing and the lower the environmental polarity, the more blue-shifted the emission; the looser the lipid packing or the stronger the hydration and the higher the environmental polarity, the more red-shifted the emission. NRLD shows blue shift in the non-polar oil core of lipid droplets and red shift in high-polarity environments such as phosphate buffer .
Cat. No.: HY-D3130
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Mem-pH is a ratiometric fluorescent probe used for measuring vesicular pH. Mem-pH inserts into the plasma membrane bilayer and localizes to the vesicular lumen, switching between its basic and acidic forms via protonation during vesicular acidification to enable ratiometric pH readout. In aqueous media, Mem-pH forms non-emissive aggregates through aggregation-induced quenching, and then depolymerizes and recovers its fluorescent activity upon binding to the plasma membrane. The excitation/emission wavelengths of the basic form of Mem-pH are 405/450-550 nm, while those of the acidic form are 488/500-700 nm .
Cat. No.: HY-D3121
CAS No.: 2763752-95-8
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HOTPy is a mitochondria-targeted fluorescent viscosity probe with aggregation-induced emission properties, which responds to microenvironmental viscosity. HOTPy can recognize the abnormal increase in mitochondrial viscosity and capture dynamic viscosity changes in acute alcoholic liver injury models at both cellular and in vivo levels. The maximum absorption peak of HOTPy in PBS solution is at 379 nm; its maximum emission peak reaches 611 nm in a high-viscosity glycerol system. HOTPy can be used for the research of acute alcoholic liver injury .
Cat. No.: HY-D3124
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Gol-SiRhoNox is a Golgi apparatus-targeted fluorescent probe with high selectivity for Fe (II), with a limit of detection of 50 nM. Gol-SiRhoNox integrates a myristoyl motif for Golgi apparatus targeting and a silicon-fused rhodamine N-oxide as the Fe (II)-sensitive moiety. Gol-SiRhoNox is non-fluorescent in its native state; Fe (II)-mediated deoxygenation of the N-oxide triggers fluorescence recovery. Its fluorescent product, Gol-SiR, has excitation/emission wavelengths of 630/665 nm and a maximum absorption wavelength of 655 nm .
Cat. No.: HY-134096
CAS No.: 78323-98-5
Synonyms: DNS-M
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Dansyl-morpholine (DNS-M) is a Fluorescent probe for lipid droplet imaging, cancer cell discrimination, and real-time tracking of lipid droplet dynamics. As a solvatochromic probe with a donor-π-acceptor structure, it relies on hydrophobic interaction for its mechanism of action: its good lipophilicity, confirmed by an oil-water partition coefficient LogP = 2.35, allows it to rapidly penetrate cell membranes, and it specifically localizes to the hydrophobic core of lipid droplets; its fluorescence is strongly enhanced in the nonpolar environment of lipid droplets, while it emits very weak fluorescence in polar environments like PBS buffer, and it exhibits a bathochromic shift in emission wavelength with increasing solvent polarity. It has negligible cytotoxicity, with cell viability remaining over 95% after 24-hour incubation with 100 μM of the probe, and it possesses excellent photostability, retaining over 97% of initial fluorescence intensity after 60 continuous laser scans. For cell imaging applications, its excitation/emission wavelengths for lipid droplet labeling are Ex/Em = 405/480−540 nm, and in a simulative lipid environment O/W emulsion, it has an excitation wavelength of ~346 nm and emission wavelength of ~500 nm, giving a Stokes shift of 154 nm[1].
Cat. No.: HY-D3125
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NtHzBtd is a fluorescent probe for detecting Fe 3+. NtHzBtd is applicable to the selective fluorescent detection of Fe 3+ ions and live cell imaging studies. NtHzBtd can selectively coordinate with Fe 3+ to form a 1:1 complex, triggering chelation enhanced quenching (CHEQ) and intramolecular charge transfer (ICT) processes, which result in fluorescence turn-off, thereby enabling sensitive detection of Fe 3+ and live cell fluorescence imaging. After binding to Fe 3+, NtHzBtd reduces fluorescence intensity, exhibits a rapid response property, with a limit of detection of 0.036 μM and a response time of approximately 55 s. The detection wavelengths are Ex/Em = 334/401 nm (solution system) .
Cat. No.: HY-D3385
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AM4-66 is a fluorescent dye used for cell membrane surface labeling. AM4-66 serves as a cell membrane surface marker and can label intact plasma membranes; when used sequentially with fluid-phase endocytosis markers such as fluorescent dextran, it does not stain dextran-positive intracellular structures that have detached from the cell surface, thus confirming that these internalized membranes no longer belong to the intact cell membrane surface (Ex/Em = 510/750 nm) .
Cat. No.: HY-D3075
CAS No.: 2490504-23-7
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TPE-2E N-oxide is a fluorescent probe used for hypoxia detection and tumor hypoxia imaging. Due to its active intramolecular motion, TPE-2E N-oxide shows no fluorescence in aqueous solution. In hypoxic environments, its N-oxide group undergoes two-electron reduction by CYP450 reductase and other heme protein reductases expressed under hypoxia, forming hydrophobic aggregates that trigger aggregation-induced emission through the restriction of intramolecular motion. TPE-2E N-oxide exhibits selective lipid droplet localization in cells. The absorption wavelength of TPE-2E N-oxide in dichloromethane is 313 nm; in a 99% hexane/dichloromethane mixed system, its excitation wavelength is 330 nm and emission wavelength is 460 nm. For in vitro cell imaging, excitation is performed at 405 nm, with an emission wavelength range of 430-560 nm .
Cat. No.: HY-D3567
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AF647 Cadaverine is a far-red fluorescent polarity tracer. AF647 Cadaverine possesses a primary amine group that enables covalent labeling of carboxylic acids via carbodiimide activation or covalent labeling of glutamine residues through transamidation, rendering it applicable for bioconjugation, cell imaging, and blood-brain barrier permeability assays (Ex/Em = 651/670 nm) .
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Protocols
Targeted therapy! The Capetin Prize winning "Click Chemistry" can be used like this!Targeted therapy! The Nobel Prize winning "Click Chemistry" can be used like this!A suitable model is crucial in drug screening experiments. Organs can mimic the three-dimensional functional structure of internal organs, have similar spatial organization to corresponding organs, maintain some key characteristics, and reproduce some physiological functions. They are widely used for modeling and personalized drug screening physiological functions.2025-02-06
