AF647 Cadaverine
AF647 Cadaverine is a far-red fluorescent polarity tracer. AF647 Cadaverine possesses a primary amine group that enables covalent labeling of carboxylic acids via carbodiimide activation or covalent labeling of glutamine residues through transamidation, rendering it applicable for bioconjugation, cell imaging, and blood-brain barrier permeability assays (Ex/Em = 651/670 nm).
For research use only. We do not sell to patients.
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (The following is our recommended protocol. This protocol is for guidance only and should be modified according to your specific needs).
1. Preparation: Dissolve your carboxyl-containing biomolecule in a suitable buffer (e.g., PBS or MES, pH 6.0-7.2).
2. Activation: Add 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and sulfo-NHS to activate the carboxyl groups for 1 hour at room temperature.
3. Reaction: Add AF647 Cadaverine dissolved in sodium carbonate buffer (pH ~9).
4. Incubation: React overnight at room temperature in the dark.
5. Purification: Dialyse or use gel filtration (such as Bio-Gel P30) to remove unreacted dye.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 (male and female, 5-7 months of age)[1]
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Dosage:400 mM
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Administration:i.v. via retro-orbital vein; single administration per imaging session
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Result:Detected a 12-fold increase in parenchymal fluorescence in line-scan-induced capillary injury models compared to pre-injection baseline.
Showed no significant increases in parenchymal fluorescence at 5 minutes or 2 days post-single pericyte ablation, indicating no detectable blood-brain barrier leakage.
Chemical Information
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SMILES
[AF647 Cadaverine]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
AF647 Cadaverine Related Classifications
HY-D3567 Related Classifications
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