Gol-SiRhoNox
Gol-SiRhoNox is a Golgi apparatus-targeted fluorescent probe with high selectivity for Fe (II), with a limit of detection of 50 nM. Gol-SiRhoNox integrates a myristoyl motif for Golgi apparatus targeting and a silicon-fused rhodamine N-oxide as the Fe (II)-sensitive moiety. Gol-SiRhoNox is non-fluorescent in its native state; Fe (II)-mediated deoxygenation of the N-oxide triggers fluorescence recovery. Its fluorescent product, Gol-SiR, has excitation/emission wavelengths of 630/665 nm and a maximum absorption wavelength of 655 nm.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 分子式: C47H68N4O5Si
- 分子量:797.15
-
保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
生物活性
-
化学情報
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
生物活性
製品説明
体外実験
1. Stock Solution Preparation
1.1 Solvent: DMSO.
1.2 Concentration recommendation: 1 mM.
2. Working Solution Preparation
2.1 Diluent: HBSS.
2.2 Working concentration: 5 μM.
2.3 Notes: Adjust the working solution concentration as needed; prepare fresh before use.
3. Staining Procedure
3.1 Sample Type Description
3.1.1 Adherent cells (HepG2 cells, SH-SY5Y neuroblastoma cells)[1].
3.2 Incubation Conditions
3.2.1 For adherent cells: Incubate cells with 5 μM Gol-SiRhoNox at 37°C for 30 min.
3.3 Washing Steps
3.3.1 For adherent cells: Before adding the probe, carefully wash the cells 3 times with an appropriate buffer to remove extracellular Fe (II).
4. Control Setup
4.1 Positive control: Before probe incubation, incubate cells in FBS-free Modified Essential Medium (MEM) with 100 μM Fe (II) (added as Fe (NH4)2 (SO4)2·6H2O) at 37°C for 30 min.
4.2 Negative control: Incubate cells with Gol-SiRhoNox in a system containing 1 mM 2,2'-Bipyridine (HY-D0020).
4.3 Blank control: Do not perform Fe (II) supplementation treatment on cells before probe incubation.
5. Detection and Analysis
5.1 Instrument type: Fluorescence microscope.
5.2 Excitation/emission wavelength: 630 nm/665 nm (Cy5 filter set).
5.3 Result Analysis
5.3.1 Fluorescence localization: Fe (II) mainly distributes in the Golgi apparatus in normal cells, while its localization shifts to lysosomes in systems with protein sorting defects.
5.3.2 Changes in fluorescence intensity: Fluorescence is generated by the interaction with labile Fe (II) in the Golgi apparatus; cells pretreated with Fe (II) show increased fluorescence intensity; cells treated with Fe (II) chelators show decreased fluorescence intensity; the Golgi fluorescence intensity of VPS35-knockdown cells is lower than that of wild-type cells, and it can be restored after treatment with R55.
5.3.3 Color change: Magenta fluorescence is observable under the Cy5 filter set.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
-
分子量 797.15
-
分子式 C47H68N4O5Si
-
SMILES
CCCCCCCCCCCCCC(NCCNC(C1=CC2=C(C(OC23C4=CC=C(C=C4[Si](C)(C5=C3C=CC([N+](CC)(CC)[O-])=C5)C)N(CC)CC)=O)C=C1)=O)=O
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Gol-SiRhoNox 関連分類
HY-D3124 関連分類
Powered by Bioz