URAT1 inhibitor 3

Name: URAT1 inhibitor 3
Brand: MedChemExpress
SKU: HY-151432
Rating: 4.5 (1 reviews)

Cat. No.: HY-151432 Purity: 98.02%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

URAT1 inhibitor 3 is a potent, orally active, selective URAT1 inhibitor with an IC₅₀ value of 0.8 nM. URAT1 inhibitor 3 has urate-lowering efficacy. URAT1 inhibitor 3 can be used in research of gout and hyperuricemi.

For research use only. We do not sell to patients.

URAT1 inhibitor 3 Chemical Structure

CAS No. : 2850331-30-3

Size	Price	Stock	Quantity
5 mg	USD 240	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 390	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 750	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1150	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1750	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

IC50: 0.8 nM (URAT1)^[1]

In Vitro

URAT1 inhibitor 3 (0-400 μM; 24 and 72 h; HepG2 and HK2 cells) has low toxicity and inhibits cell viability at high concentrations^[1].
URAT1 inhibitor 3 (0.01-100 μM) has lower inhibition on urate excretion transporters with IC₅₀ values of 10.16 μM and 4.04 μM of OAT1 and ABCG2, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	HepG2 and HK2 cells
Concentration:	0, 100, 200, 300, and 400 μM
Incubation Time:	24 and 72 hours
Result:	Had little cytotoxicity at 24 h and inhibition rate of 34.75% and 35.9% of HepG2 and HK2 cells, respectively.

In Vivo

URAT1 inhibitor 3 (1-4 mg/kg; i.g.; once) has urate lowering efficacy in a mouse model of hyperuricemia^[1].
URAT1 inhibitor 3 (50 mg/kg; i.g.; daily, for 14 d) has no hepatic and renal toxicities in mice^[1].
URAT1 inhibitor 3 (50 mg/kg; i.g.; once) induces GSH depletion in Kunming mice with hyperuricemia model^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Kunming (KM) mice with hyperuricemia model^[1]
Dosage:	1, 2, and 4 mg/kg
Administration:	Oral gavage; once
Result:	Decreased the serum urate levels in a dose-dependent manner.

Animal Model:	Male Kunming mice^[1]
Dosage:	50 mg/kg
Administration:	Oral gavage; daily, for 14 days
Result:	Did not cause renal toxicity.

Animal Model:	Male Kunming mice with hyperuricemia model^[1]
Dosage:	50 mg/kg
Administration:	Oral gavage; once
Result:	Decreased the serum GSH levels from 42.23 μM to 20.39 μM.

Molecular Weight

307.13

Formula

C₁₄H₈Cl₂N₂O₂

CAS No.

2850331-30-3

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(C1=CC(Cl)=C(O)C(Cl)=C1)N2C=CC3=CC=CN=C32

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (325.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2560 mL	16.2798 mL	32.5595 mL
5 mM	0.6512 mL	3.2560 mL	6.5119 mL
10 mM	0.3256 mL	1.6280 mL	3.2560 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 10 mg/mL (32.56 mM); Clear solution

This protocol yields a clear solution of ≥ 10 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (100.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.02%

Select Batch:

Data Sheet (281 KB) SDS (251 KB)

COA (260 KB) HNMR (110 KB) LCMS (99 KB)

Handling Instructions (2659 KB)

References

[1]. Zhao Z, et, al. Discovery of novel benzbromarone analogs with improved pharmacokinetics and benign toxicity profiles as antihyperuricemic agents. Eur J Med Chem. 2022 Nov 15;242:114682. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2560 mL	16.2798 mL	32.5595 mL	81.3988 mL
	5 mM	0.6512 mL	3.2560 mL	6.5119 mL	16.2798 mL
	10 mM	0.3256 mL	1.6280 mL	3.2560 mL	8.1399 mL
	15 mM	0.2171 mL	1.0853 mL	2.1706 mL	5.4266 mL
	20 mM	0.1628 mL	0.8140 mL	1.6280 mL	4.0699 mL
	25 mM	0.1302 mL	0.6512 mL	1.3024 mL	3.2560 mL
	30 mM	0.1085 mL	0.5427 mL	1.0853 mL	2.7133 mL
	40 mM	0.0814 mL	0.4070 mL	0.8140 mL	2.0350 mL
	50 mM	0.0651 mL	0.3256 mL	0.6512 mL	1.6280 mL
	60 mM	0.0543 mL	0.2713 mL	0.5427 mL	1.3566 mL
	80 mM	0.0407 mL	0.2035 mL	0.4070 mL	1.0175 mL
	100 mM	0.0326 mL	0.1628 mL	0.3256 mL	0.8140 mL