URAT1 inhibitor 3
Based on 1 Customer Validation
URAT1 inhibitor 3 is a potent, orally active, selective URAT1 inhibitor with an IC50 value of 0.8 nM. URAT1 inhibitor 3 has urate-lowering efficacy. URAT1 inhibitor 3 can be used in research of gout and hyperuricemi.
For research use only. We do not sell to patients.
- Purity: 98.02%
- CAS No.: 2850331-30-3
- Formula: C14H8Cl2N2O2
- Molecular Weight:307.13
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
IC50: 0.8 nM (URAT1)[1]
URAT1 inhibitor 3 (0-400 μM; 24 and 72 h; HepG2 and HK2 cells) has low toxicity and inhibits cell viability at high concentrations[1].
URAT1 inhibitor 3 (0.01-100 μM) has lower inhibition on urate excretion transporters with IC50 values of 10.16 μM and 4.04 μM of OAT1 and ABCG2, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HepG2 and HK2 cells
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Concentration:0, 100, 200, 300, and 400 μM
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Incubation Time:24 and 72 hours
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Result:Had little cytotoxicity at 24 h and inhibition rate of 34.75% and 35.9% of HepG2 and HK2 cells, respectively.
URAT1 inhibitor 3 (50 mg/kg; i.g.; daily, for 14 d) has no hepatic and renal toxicities in mice[1].
URAT1 inhibitor 3 (50 mg/kg; i.g.; once) induces GSH depletion in Kunming mice with hyperuricemia model[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Kunming (KM) mice with hyperuricemia model[1]
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Dosage:1, 2, and 4 mg/kg
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Administration:Oral gavage; once
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Result:Decreased the serum urate levels in a dose-dependent manner.
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Animal Model:Male Kunming mice[1]
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Dosage:50 mg/kg
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Administration:Oral gavage; daily, for 14 days
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Result:Did not cause renal toxicity.
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Animal Model:Male Kunming mice with hyperuricemia model[1]
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Dosage:50 mg/kg
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Administration:Oral gavage; once
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Result:Decreased the serum GSH levels from 42.23 μM to 20.39 μM.
Chemical Information
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CAS No. 2850331-30-3
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Appearance Solid
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Molecular Weight 307.13
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Formula C14H8Cl2N2O2
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Color Off-white to light yellow
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SMILES
O=C(C1=CC(Cl)=C(O)C(Cl)=C1)N2C=CC3=CC=CN=C32
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (325.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (281 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2560 mL | 16.2798 mL | 32.5595 mL | 81.3988 mL |
| 5 mM | 0.6512 mL | 3.2560 mL | 6.5119 mL | 16.2798 mL | |
| 10 mM | 0.3256 mL | 1.6280 mL | 3.2560 mL | 8.1399 mL | |
| 15 mM | 0.2171 mL | 1.0853 mL | 2.1706 mL | 5.4266 mL | |
| 20 mM | 0.1628 mL | 0.8140 mL | 1.6280 mL | 4.0699 mL | |
| 25 mM | 0.1302 mL | 0.6512 mL | 1.3024 mL | 3.2560 mL | |
| 30 mM | 0.1085 mL | 0.5427 mL | 1.0853 mL | 2.7133 mL | |
| 40 mM | 0.0814 mL | 0.4070 mL | 0.8140 mL | 2.0350 mL | |
| 50 mM | 0.0651 mL | 0.3256 mL | 0.6512 mL | 1.6280 mL | |
| 60 mM | 0.0543 mL | 0.2713 mL | 0.5427 mL | 1.3566 mL | |
| 80 mM | 0.0407 mL | 0.2035 mL | 0.4070 mL | 1.0175 mL | |
| 100 mM | 0.0326 mL | 0.1628 mL | 0.3256 mL | 0.8140 mL |
URAT1 inhibitor 3 Related Classifications
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