V-ATPase-IN-2 

V-ATPase-IN-2 is an inhibitor targeting the a-c subunits of vacuolar H⁺-ATPase (V-ATPase), with an EC₅₀ of 8.449 μg/mL against Phytophthora capsici. V-ATPase-IN-2 causes significant vacuolar enlargement and ultrastructural damage in Phytophthora capsici cells, and also inhibits sporangium formation, zoospore release, and cyst germination of this pathogen. V-ATPase-IN-2 can be used in studies related to Phytophthora capsici infection^[1].

V-ATPase-IN-2 (compound 4j) potently inhibits mycelial growth of Phytophthora capsici, with an EC₅₀ value of 8.449 μg/mL. This compound exhibits broad-spectrum inhibitory activity against other phytopathogenic fungi and retains activity against some flutolanil-resistant mutant strains^[1].
V-ATPase-IN-2 (5-20 μg/mL) inhibits the activity of Phytophthora capsici V-ATPase in a dose-dependent manner, with an inhibition rate of 57.72% at the concentration of 20 μg/mL^[1].
V-ATPase-IN-2 (10-20 μg/mL; 2 days of dark incubation + 2 days of light incubation) potently inhibits sporangium formation in Phytophthora capsici^[1].
V-ATPase-IN-2 (10-20 μg/mL; 30 min incubation at 4 °C followed by incubation at 25 °C for zoospore release) inhibits zoospore release of Phytophthora capsici^[1].
V-ATPase-IN-2 (10-20 μg/mL; 6 h incubation at 25 °C in the dark) inhibits sporangiospore germination of Phytophthora capsici^[1].
V-ATPase-IN-2 (20 μg/mL; incubated until colonies reach a diameter of ~4 cm²) disrupts the cellular ultrastructure of Phytophthora capsici hyphae by inducing significant vacuolar enlargement^[1].
V-ATPase-IN-2 forms stable interactions with the interface between subunits a and c of Phytophthora capsici V-ATPase, in which the Lys847 and Phe764 residues play key roles, supporting its function as a V-ATPase inhibitor^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

514.42

C₂₂H₂₀F₆N₆O₂

O=C(CN1N=C(C=C1C)C(F)(F)F)N2CCN(CC2)C3=NC=NC(C4=CC=C(C=C4)OC(F)(F)F)=C3

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Lu X, et al. Computationally and Target-Guided Discovery of Trifluoromethyl-Pyrazole Derivatives Targeting the a–c Subunit Interface of V-ATPase in Phytophthora capsici. J Agric Food Chem. 2026 May 20;74(20).