2. Cell Cycle/DNA Damage
  3. IRE1
  4. 3,6-DMAD hydrochloride

3,6-DMAD hydrochloride, an acridine derivative, is a potent IRE1α-XBP1s pathway inhibitor. 3,6-DMAD hydrochloride promotes IL-6 secretion via the IRE1α-XBP1s pathway. 3,6-DMAD hydrochloride inhibits IRE1α oligomerization and endoribonuclease (RNase) activity. 3,6-DMAD hydrochloride can be used for research of cancer.

For research use only. We do not sell to patients.

3,6-DMAD hydrochloride Chemical Structure

3,6-DMAD hydrochloride Chemical Structure

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3,6-DMAD hydrochloride Related Antibodies

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Description

3,6-DMAD hydrochloride, an acridine derivative, is a potent IRE1α-XBP1s pathway inhibitor. 3,6-DMAD hydrochloride promotes IL-6 secretion via the IRE1α-XBP1s pathway. 3,6-DMAD hydrochloride inhibits IRE1α oligomerization and endoribonuclease (RNase) activity. 3,6-DMAD hydrochloride can be used for research of cancer[1][2].

In Vitro

3,6-DMAD hydrochloride (0-6 μM; 24 h; RPMI 8226 and MM1.R human MM cells) has cytotoxicity against MM cell lines[1].
3,6-DMAD hydrochloride (0-30 μM; 14 h; HT1080 cells treated with Tg (0.3 μM)) inhibits XBP1 splicing (XBP1s) in a dose dependent manner[1].
3,6-DMAD hydrochloride (0.1-500 μM; 14 h; HT1080 cells treated with Tg (0.3 μM)) inhibits IRE1α endonuclease activity[1].
3,6-DMAD hydrochloride (1-60 μM; 2 h; HEK293 cells) inhibits IRE1α oligomerization and IRE1α-GFP foci formation[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

3,6-DMAD hydrochloride Related Antibodies

Cell Viability Assay[1]

Cell Line: RPMI 8226 and MM1.R human MM cells
Concentration: 0, 0.5, 1, 2, 3, 4, 5 and 6 μM
Incubation Time: 24 hours
Result: Inhibited cell survival rate in a dose dependent manner.

Western Blot Analysis[1]

Cell Line: HT1080 cells treated with Tg (0.3 μM)
Concentration: 0, 5, 10 and 30 μM
Incubation Time: 14 hours
Result: Showed XBP1s inhibition at as low as 0.5 μM.
In Vivo

3,6-DMAD hydrochloride (10 mg/kg; i.p.; three times every 12 hours, for 84 hours; NOD Scid mice with RPMI 8226 xenograft) has inhibition of XBP1 splicing in vivo[1].
3,6-DMAD hydrochloride (10 mg/kg; 24 h; i.p.; every 48 hours, for 12 days; NOD Scid mice with RPMI 8226 xenograft) suppresses multiple myeloma xenograft growth in vivo[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: NOD Scid mice (4-6 weeks) with RPMI 8226 xenograft[1]
Dosage: 10 mg/kg
Administration: Intraperitoneal injection; three times every 12 hours, for 84 hours
Result: Inhibited XBP1-luciferase activity in NOD Scid mice with RPMI 8226 xenograft.
Animal Model: NOD Scid mice (4-6 weeks) with RPMI 8226 xenograft[1]
Dosage: 10 mg/kg
Administration: Intraperitoneal injection; every 48 hours, for 12 days
Result: Inhibited tumor growth in NOD Scid mice with RPMI 8226 xenograft.
Formula

C22H31N5
Appearance

Solid

Color

Yellow to orange

SMILES

CN(C1=CC=C2C(N=C3C=C(C=CC3=C2NCCCN(C)C)N(C)C)=C1)C.[x HCl]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

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Purity & Documentation

Purity: ≥99.0%

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3,6-DMAD hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3,6-DMADIRE1Inositol requiring enzyme 1RPMI 8226 cellIRE1α oligomerizationendoribonucleaseacridine derivativeanticancerInhibitorinhibitorinhibit

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