1. Cell Cycle/DNA Damage
  2. Eukaryotic Initiation Factor (eIF)
    Virus Protease
  3. 4E2RCat


Cat. No.: HY-100733 Purity: >98.0%
Handling Instructions

4E2RCat is an inhibitor of eIF4E-eIF4G interaction with an IC50 of 13.5 μM.

For research use only. We do not sell to patients.

4E2RCat Chemical Structure

4E2RCat Chemical Structure

CAS No. : 432499-63-3

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10 mM * 1 mL in DMSO USD 397 In-stock
Estimated Time of Arrival: December 31
1 mg USD 132 In-stock
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10 mg USD 600 In-stock
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50 mg USD 1800 In-stock
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Based on 1 publication(s) in Google Scholar

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4E2RCat is an inhibitor of eIF4E-eIF4G interaction with an IC50 of 13.5 μM.

IC50 & Target

IC50: 13.5 μM (eIF4E-eIF4G)[1]

In Vitro

4E2RCat prevents the interaction between eIF4E (the cap-binding protein) and eIF4G (a large scaffolding protein), inhibiting cap-dependent translation. It significantly decreases human coronavirus 229E (HCoV-229E) replication, reducing the percentage of infected cells and intra- and extracellular infectious virus titers. 4E2RCat inhibits cap-dependent translation in a dose-dependent manner. 4E2RCat inhibits cap-dependent FF translation but not EMCV IRES-driven Ren translation. 4E2RCat inhibits coronavirus replication in a dose- and time-dependent manner[1].

In Vivo

4E2RCat inhibits protein synthesis in vivo and it is not a consequence of increased cell death[1].

Molecular Weight









Room temperature in continental US; may vary elsewhere.

Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 15.5 mg/mL (34.00 mM; Need ultrasonic and warming)

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1933 mL 10.9666 mL 21.9332 mL
5 mM 0.4387 mL 2.1933 mL 4.3866 mL
10 mM 0.2193 mL 1.0967 mL 2.1933 mL
*Please refer to the solubility information to select the appropriate solvent.
Cell Assay

L132 cells are treated with 12.5 μM 4E2RCat for the indicated times and are processed for annexin V/propidium iodide staining. To this end, cell medium is collected. Cells are ished with 1 mL PBS, which is collected as well, and trypsinized in 200 μL 0.05% trypsin-EDTA. Cells are pooled with previously collected supernatants and spun for 2 min at 2,000 rpm and 4°C. The cell pellet is ished with 2 mL cold PBS, followed by another spin. After the second spin, the cell pellet is resuspended in 100 μL annexin V binding buffer and propidium iodide added to a final concentration of 5 μg/mL. After the addition of 5 μL annexin V-fluorescein isothiocyanate, samples are incubated for 15 min in the dark at room temperature and diluted. Fluorescence-activated cell sorter (FACS) analyses are performed using a FACScan instrument[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

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4E2RCatEukaryotic Initiation Factor (eIF)Virus ProteaseAutophagyInhibitorinhibitorinhibit

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