1. Apoptosis
  2. Apoptosis
  3. Adarotene

Adarotene (Synonyms: ST1926)

Cat. No.: HY-14808 Purity: 99.18%
Handling Instructions

Adarotene is an effective apoptosis inducer, which surprisingly produces DNA damage and exhibites a potent antiproliferative activity on a large panel of human tumor cells.

For research use only. We do not sell to patients.

Adarotene Chemical Structure

Adarotene Chemical Structure

CAS No. : 496868-77-0

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10 mM * 1 mL in DMSO USD 92 In-stock
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5 mg USD 84 In-stock
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10 mg USD 156 In-stock
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50 mg USD 492 In-stock
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Customer Review

Based on 7 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Adarotene purchased from MCE. Usage Cited in: Cell Physiol Biochem. 2017;41(2):519-529.

    Arithmetic means±SEM (n=10) of erythrocyte annexin-V-binding following incubation for 48 hours to Ringer solution without (white bar) or with (black bars) Adarotene (3-9 µM) or solvant DMSO alone (grey bar).

    Adarotene purchased from MCE. Usage Cited in: Biomed Pharmacother. 2020 Jun 8;128:110291.

    Western blot analysis of p-AMPKα, p-mTOR and p-P70S6K in ST1962 (2.5, 5 and 10 μM)-treated glioma cell lines for 24 h. AMPKα activation is markedly induced by ST1926 in a dose-dependent manner; phosphorylated mechanistic target of rapamycin (mTOR) and P70S6K expression levels are obviously down-regulated.

    Adarotene purchased from MCE. Usage Cited in: Biomed Pharmacother. 2020 Jun 8;128:110291.

    Effects of ST1926 on retinoic acid (RA) pathway. Glioma cells are treated with ST1926 (2.5, 5 and 10 μM) for 24 h, immunofluorescence staining of CRABP2.
    • Biological Activity

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    Description

    Adarotene is an effective apoptosis inducer, which surprisingly produces DNA damage and exhibites a potent antiproliferative activity on a large panel of human tumor cells.

    In Vitro

    Adarotene causes a dose-dependent growth inhibition in a large panel of human tumor cell lines with IC50 ranging from 0.1 to 0.3 µM. Adarotene causes cell accumulation in G1/S or S phase of cell cycle depending on tumor cells IGROV-1 and DU145[1]. Adarotene is apoptotic and cytotoxic on a large spectrum of cancerous and leukemic cells, including freshly isolated AML blasts in primary culture. The molecular target of ST1926 apoptotic activity in myeloid leukemia cells is similar to the ligand-binding domain of RARγ. Adarotene treatment of cells results in rapid accumulation of intracellular calcium[2].

    In Vivo

    Adarotene (15, 20 mg/kg, p.o.) causes a significant tumor growth inhibition in a human ovarian carcinoma, A2780/DX, and in a human melanoma, MeWo, growing in nude mice[1]. Adarotene (30, 40 mg/kg, p.o.) results in a significant and dose-dependent increase in the life span of NB4-bearing SCID mice without overt toxicity[2].

    Molecular Weight

    374.47

    Formula

    C₂₅H₂₆O₃

    CAS No.

    496868-77-0

    SMILES

    O=C(O)/C=C/C1=CC=C(C2=CC=C(O)C(C3(C4)CC5CC4CC(C5)C3)=C2)C=C1

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 25 mg/mL (66.76 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.6704 mL 13.3522 mL 26.7044 mL
    5 mM 0.5341 mL 2.6704 mL 5.3409 mL
    10 mM 0.2670 mL 1.3352 mL 2.6704 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (6.68 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (6.68 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Cell Assay
    [2]

    Briefly, cells (1×107/mL) are loaded with 1 μM FURA-2 at 37°C in the dark for 30 minutes, washed twice, resuspended in phosphate-buffered saline (PBS) containing 1.26 mM CaCl2 at 106 cells/mL and then used for the experiments. Dual excitation, alternating at 340 nm and 380 nm, is provided by a spectrophotofluorometer equipped with 2 excitation monochromators, and emission is fixed at 480 nm. The temperature is set at 37°C±1°C. In some experiments, to eliminate extracellular calcium, cells preloaded with FURA-2 are resuspended in PBS without Ca2+, and 0.5 mM EGTA (ethylene glycol tetraacetic acid) is added to each sample prior to addition of the appropriate stimulus.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [2]

    NB4 cells (3×106) are intraperitoneally inoculated in SCID mice (8 mice/group). ST1926 is dissolved in cremophor/ethanol 1:1 solution, and diluted 1:10 in PBS at the concentration of 50 mg/kg; the doses of 30 mg/kg and 40 mg/kg are then prepared by appropriate dilutions in the same vehicle. ATRA is dissolved in the dark in Cremophor EL and kept magnetically stirred; the solution is then diluted 1:10 in PBS at the final concentration of 40 mg/kg. Both compounds are administered intraperitoneally and orally twice per day for 3 weeks starting from the day after cell inoculation, in a volume of 10 mL/kg. During treatments body weight and lethality are registered.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Purity: 99.18%

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    Keywords:

    AdaroteneST1926ST 1926ST-1926ApoptosisInhibitorinhibitorinhibit

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