Art. -Nr.: HY-16271

Kevetrin hydrochloride

Name: Kevetrin hydrochloride
Brand: MedChemExpress
SKU: HY-16271
Price: 49.00 USD
Availability: InStock
Rating: 5 (4 reviews)

(Synonyms: 4-Isothioureidobutyronitrile hydrochloride; thioureidobutyronitrile hydrochloride; thioureido butyronitrile hydrochloride)

4 Cited Publications

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Based on 4 publication(s) in Google Scholar

Kevetrin hydrochloride is a potent activator of p53, induces apoptosis in TP53 wild-type and mutant acute myeloid leukemia cells. Kevetrin a preferential cytotoxic activity against blast cells.

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Reinheit : 98.0%
CAS. Nr.: 66592-89-0
Formel: C₅H₁₀ClN₃S
Molecular Weight:179.67

Speicherung:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Kevetrin hydrochloride Chemical Structure

CAS. Nr.: 66592-89-0

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Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Kevetrin hydrochloride is a potent activator of p53, induces apoptosis in TP53 wild-type and mutant acute myeloid leukemia cells. Kevetrin a preferential cytotoxic activity against blast cells^[1]^[2].

In Vitro

Kevetrin hydrochloride (85, 170, 340 μM; 6 h) significantly inhibits KASUMI-1 cells growth in a dose-dependent manner, without affecting MOLM-13 cells^[1].
Kevetrin hydrochloride (340 μM; 6 h) induces metallothionein (MT) expression in acute myeloid leukemia (AML) cells, and also down-regulates genes in relation to p53 activity, the regulator of WNT/β-catenin signaling forkhead box K2 and the transcription factor signal transducer and activator of transcription 5A (STAT5A)^[1].
Kevetrin hydrochloride (340 μM; 24 h) induces apoptosis on KASUMI-1 cell line, without leading cell cycle alteration^[1].
Kevetrin hydrochloride (100, 200, 400 μM; 48 h) increases the mRNA and protein level of p53 and induces p21 protein production in A2780 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

MOLM-13 and KASUMI-1 cells
Concentration:

85, 170 and 340 µM
Incubation Time:

6 h, 6 h + 66 h wash-out (wo,×1), 6 h + 66 h wo (×2), 6 h + 66 h wo (×3)
Result:

Only inhibited the cell viability of KASUMI-1 cells and decreased cells viability in a dose- and time-dependent manner.

Apoptosis Analysis^[1]

Cell Line:

MOLM-13, KASUMI-1, TP53-wt OCI-AML3, and TP53-mutant NOMO-1 cells
Concentration:

85, 170 and 340 µM
Incubation Time:

24, 48, and 72 hours
Result:

Induced KASUMI-1 apoptosis at 340 μM on 24 hours and inhibited MOLM-13 at 340 μM on 48 h.

Cell Cycle Analysis^[1]

Cell Line:

MOLM-13, KASUMI-1, TP53-wt OCI-AML3, and TP53-mutant NOMO-1 cells
Concentration:

340 µM
Incubation Time:

24, and 48 hours
Result:

Arrested cell cycle of OCI-AML3 and NOMO-1 cells at G0/G1 phase, without altering cell cycle of MOLM-13 and KASUMI-1 cells.

In Vivo

Kevetrin hydrochloride (150-200 mg/kg; i.p.; 20 d) induces ~40% cell death in OV-90 or OVCAR-3 xenograft tumors, also inhibits tumor growth and extents survival time of mice with tumor xenograft mode^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

A2780 xenograft tumor model in nude mice^[2]
Dosage:

200 mg/kg
Administration:

Intraperitoneal injection; 3 times per week, for 20 days
Result:

Inhibited tumor growth and suppressed the tumor volume.

Animal Model:

SKOV-3 xenograft ascites model in mice^[2]
Dosage:

150 mg/kg
Administration:

Intraperitoneal injection
Result:

Prolonged the survival time of mice, and maintained 100% survival rate more over 35 days.

Related Protocols :

Chemical Information

CAS. Nr. 66592-89-0
Appearance Solid
Molecular Weight 179.67
Formel C₅H₁₀ClN₃S
Color White to off-white
SMILES

NC(SCCCC#N)=N.[H]Cl
Synonyms

4-Isothioureidobutyronitrile hydrochloride; thioureidobutyronitrile hydrochloride; thioureido butyronitrile hydrochloride
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (4)

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Journal Impact Factor

J Ethnopharmacol

Naoxintong capsule accelerates mitophagy in cerebral ischemia-reperfusion injury via TP53/PINK1/PRKN pathway based on network pharmacology analysis and experimental validation. [Abstract]

2025 Jan 10:336:118721. PMID: 39173723
Int Immunopharmacol

Kaempferol inhibits atherosclerotic plaque development via dual-targeting of p53-p21-p16 senescence pathway and Nrf2/HO-1/NQO1 antioxidant mechanism: Insights from combined in vivo and in vitro research. [Abstract]

2025 Sep 20:166:115587. PMID: 40976052
Invest Ophthalmol Vis Sci

Corneal Sensory Denervation Causes Epithelial Ferroptosis and Delayed Healing in Mice. [Abstract]

2025 Jun 2;66(6):28. PMID: 40488714
J Bioenerg Biomembr

Total favonoids of Desmodium Styracifolium relieve renal ischemia-reperfusion injury by suppressing ferroptosis through P53/SLC7A11/GPX4 signaling pathway : (Running title) TFDS relieved renal ischemia-reperfusion injury. [Abstract]

2025 Oct 9. PMID: 41065914

Lösungsmittel & Löslichkeit

In Vitro:

H₂O : 100 mg/mL (556.58 mM; Need ultrasonic)

DMSO : ≥ 40 mg/mL (222.63 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.5658 mL

27.8288 mL

55.6576 mL

5 mM

1.1132 mL

5.5658 mL

11.1315 mL

10 mM

0.5566 mL

2.7829 mL

5.5658 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (13.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (13.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (13.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (556.58 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Napolitano R, et al. Kevetrin induces apoptosis in TP53 wildtype and mutant acute myeloid leukemia cells. Oncol Rep. 2020 Oct;44(4):1561-1573. [Content Brief]

[2]. Kumar A, et al. Abstract 3221: kevetrin induces p53-dependent and independKumar A, et al. Abstract 3221: kevetrin induces p53-dependent and independent cell cycle arrest and apoptosis in ovarian cancer cell lines representing heterogeneous histologies. Cancer Research. 2017. 77(13 Supplement), 3221-3221.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	5.5658 mL	27.8288 mL	55.6576 mL	139.1440 mL
	5 mM	1.1132 mL	5.5658 mL	11.1315 mL	27.8288 mL
	10 mM	0.5566 mL	2.7829 mL	5.5658 mL	13.9144 mL
	15 mM	0.3711 mL	1.8553 mL	3.7105 mL	9.2763 mL
	20 mM	0.2783 mL	1.3914 mL	2.7829 mL	6.9572 mL
	25 mM	0.2226 mL	1.1132 mL	2.2263 mL	5.5658 mL
	30 mM	0.1855 mL	0.9276 mL	1.8553 mL	4.6381 mL
	40 mM	0.1391 mL	0.6957 mL	1.3914 mL	3.4786 mL
	50 mM	0.1113 mL	0.5566 mL	1.1132 mL	2.7829 mL
	60 mM	0.0928 mL	0.4638 mL	0.9276 mL	2.3191 mL
	80 mM	0.0696 mL	0.3479 mL	0.6957 mL	1.7393 mL
	100 mM	0.0557 mL	0.2783 mL	0.5566 mL	1.3914 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Bioz

Keywords

Kevetrin hydrochloride

4 Cited Publications

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Kevetrin hydrochloride

Publications Citing Use of MedChemExpress (MCE) Kevetrin hydrochloride