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Lith-O-Asp 

Cat. No.: HY-112415 Purity: >98.0%
Handling Instructions

Lith-O-Asp is a sialytransferase (ST) inhibitor, with IC50s of 12-37 μM.

For research use only. We do not sell to patients.

Lith-O-Asp Chemical Structure

Lith-O-Asp Chemical Structure

CAS No. : 881179-02-8

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 605 In-stock
Estimated Time of Arrival: December 31
5 mg USD 550 In-stock
Estimated Time of Arrival: December 31
10 mg USD 850 In-stock
Estimated Time of Arrival: December 31
25 mg USD 1750 In-stock
Estimated Time of Arrival: December 31
50 mg USD 2750 In-stock
Estimated Time of Arrival: December 31
100 mg USD 3950 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Lith-O-Asp is a sialytransferase (ST) inhibitor, with IC50s of 12-37 μM.

IC50 & Target

IC50: 12-37 μM (ST)[1].

In Vitro

The results indicate that Lith-O-Asp shows no apparent growth inhibition effect toward different cancer cell lines at the tested doses of 10, 30, and 60 μM. By in vitro activity assay, it is revealed that the ability of Lith-O-Asp to inhibit the activities of ST3Gal I, ST3Gal III, and ST6GalI. The IC50 values ranged from 12 to 37 μM. Flow cytometry shows a significant decrease in the expression of cell surface a-2,3- and a-2,6-sialylated antigens on The results indicates that Lith-O-Asp decreased the activity of both a-2,3- and a-2,6-sialyltransferases, and thus inhibit the transfer of sialic acids to the targeted glycoproteins[1].

In Vivo

A significant amount of secondary metastatic cancer cells are observed in lung tissues of DMSO control mice detected using IVIS in vivo imaging system after 26 days of fat pad inoculation. However, Lith-O-Asp–treated mice show fewer lung metastases. All DMSO-treated mice confirm secondary lung metastasis, but only 3 of 8 Lith-O-Asp-treated mice show lung metastasis. Average tumor nodules per mouse are 11±9 nodules in DMSO-treated group, and 2±4 nodules in Lith-O-Asp-treated group. Additionally, significantly stronger 4T1-Luc illumination signals are shown in control mice than in those injected with Lith-O-Asp-treated cancer cells on days 7 and 9[1].

Solvent & Solubility
In Vitro: 

DMSO : ≥ 106 mg/mL (215.60 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0339 mL 10.1696 mL 20.3393 mL
5 mM 0.4068 mL 2.0339 mL 4.0679 mL
10 mM 0.2034 mL 1.0170 mL 2.0339 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

About 3x104 of the CL1-0, CL1-5, A549, and H1299 lung cancer cells are seeded on 12-well culture dishes in DMEM containing 10% FBS. Different concentrations of Lith-O-Asp (10, 30, and 60 μM) are added into each plate and incubated for 48 hr. Cell viabilities under different concentrations of compound treatment are determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. In the untreated control, 0.1% DMSO-containing medium is used[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Mice[1]
The BALB/c mice are used. Highly metastatic murine 4T1-Luc breast cancer cells (5 x105) are subcutaneously injected into the mammary fat pad of mice. Mice receive 3 mg/kg of Lith-O-Asp intraperitoneally on every other day. DMSO treatment is used as control. The growth and the spontaneous metastasis of the tumors are observed under IVIS50 in vivo imaging system with endotoxin-free luciferase substrate injection. The metastasized tumor tissues are dissected on day 26[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

491.66

Formula

C₂₈H₄₅NO₆

CAS No.

881179-02-8

SMILES

C[[email protected]@]12-;@[[email protected]](-;@C-;@C-;@[[email protected]]-;@2([H])[[email protected]](C)CCC(O)=O)([H])-;@[[email protected]@]3([H])-;@[[email protected]](-;@[[email protected]@]4(-;@[[email protected]](-;@C-;@[[email protected]](OC([[email protected]@H](N)CC(O)=O)=O)-;@C-;@C-;@4)([H])-;@C-;@C-;@3)C)([H])-;@C-;@C-;@1

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
Lith-O-Asp
Cat. No.:
HY-112415
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Lith-O-Asp

Cat. No.: HY-112415