NMS-P715

Name: NMS-P715
Brand: MedChemExpress
SKU: HY-12382
Rating: 4.5 (0 reviews)

Cat. No.: HY-12382 Purity: 99.30%: Data Sheet
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NMS-P715 is a selective, ATP-competitive inhibitor of MPS1, with an IC₅₀ of 182 nM.

For research use only. We do not sell to patients.

NMS-P715 Chemical Structure

CAS No. : 1202055-32-0

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
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Description

NMS-P715 is a selective, ATP-competitive inhibitor of MPS1, with an IC₅₀ of 182 nM.

IC₅₀ & Target^[1]

Mps1

182 nM (IC₅₀)

CK2

5.7 μM (IC₅₀)

MELK

6.01 μM (IC₅₀)

NEK6

6.02 μM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
786-0	IC₅₀	0.917 μM Compound: NMS-P715	Antiproliferative activity against human 786-0 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human 786-0 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
A-375	IC₅₀	0.796 μM Compound: NMS-P715	Antiproliferative activity against human A-375 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human A-375 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
A-431	IC₅₀	0.469 μM Compound: NMS-P715	Antiproliferative activity against human A-431 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human A-431 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
A2780	IC₅₀	1 μM Compound: 15, NMS-P715	Antiproliferative activity in human A2780 cells after 72 hrs by CellTiter-Glo assay Antiproliferative activity in human A2780 cells after 72 hrs by CellTiter-Glo assay	[PMID: 21723120]
A2780	IC₅₀	1 μM Compound: NMS-P715	Antiproliferative activity against human A2780 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human A2780 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
A498	IC₅₀	1.706 μM Compound: NMS-P715	Antiproliferative activity against human A498 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human A498 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
A549	IC₅₀	1.249 μM Compound: NMS-P715	Antiproliferative activity against human A549 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human A549 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
A549	IC₅₀	1.25 μM Compound: 1, NMS-P715	Antiproliferative activity against human A549 cells after 72 hrs by WST8 assay Antiproliferative activity against human A549 cells after 72 hrs by WST8 assay	[PMID: 23634759]
ACHN	IC₅₀	0.015 μM Compound: NMS-P715	Antiproliferative activity against human ACHN cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human ACHN cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
BJ	IC₅₀	5.473 μM Compound: NMS-P715	Antiproliferative activity against human BJ cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human BJ cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
BT-474	IC₅₀	1.157 μM Compound: NMS-P715	Antiproliferative activity against human BT-474 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human BT-474 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
BT-549	IC₅₀	1.049 μM Compound: NMS-P715	Antiproliferative activity against human BT-549 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human BT-549 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
BXPC-3	IC₅₀	2.179 μM Compound: NMS-P715	Antiproliferative activity against human BXPC-3 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human BXPC-3 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
C-33-A	IC₅₀	3.012 μM Compound: NMS-P715	Antiproliferative activity against human C-33-A cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human C-33-A cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
CAKI-1	IC₅₀	1.997 μM Compound: NMS-P715	Antiproliferative activity against human CAKI-1 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human CAKI-1 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
CAPAN-1	IC₅₀	5.224 μM Compound: NMS-P715	Antiproliferative activity against human CAPAN-1 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human CAPAN-1 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
CCRF-CEM	IC₅₀	1.432 μM Compound: NMS-P715	Antiproliferative activity against human CCRF-CEM cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human CCRF-CEM cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
COLO 205	IC₅₀	0.592 μM Compound: NMS-P715	Antiproliferative activity against human COLO 205 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human COLO 205 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
COLO 320	IC₅₀	1.284 μM Compound: NMS-P715	Antiproliferative activity against human COLO 320 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human COLO 320 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
COLO-678	IC₅₀	10 μM Compound: NMS-P715	Antiproliferative activity against human COLO-678 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human COLO-678 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
COLO-741	IC₅₀	10 μM Compound: NMS-P715	Antiproliferative activity against human COLO-741 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human COLO-741 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
Calu-3	IC₅₀	1.439 μM Compound: NMS-P715	Antiproliferative activity against human Calu-3 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human Calu-3 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
DLD-1	IC₅₀	1017 nM Compound: Chemical probe: NMS-P715	Inhibition of colony formation in human DLD-1 cells harboring Mps1 S611R mutant assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay Inhibition of colony formation in human DLD-1 cells harboring Mps1 S611R mutant assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay	[PMID: 26364596]
DLD-1	IC₅₀	164.8 nM Compound: Chemical probe: NMS-P715	Antiproliferative activity against human DLD-1 cells assessed as reduction in cell viability incubated for 5 days Antiproliferative activity against human DLD-1 cells assessed as reduction in cell viability incubated for 5 days	[PMID: 26364596]
DLD-1	IC₅₀	198 nM Compound: Chemical probe: NMS-P715	Inhibition of colony formation in human DLD-1 cells assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay Inhibition of colony formation in human DLD-1 cells assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay	[PMID: 26364596]
DU-145	IC₅₀	1.932 μM Compound: NMS-P715	Antiproliferative activity against human DU-145 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human DU-145 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
EKVX	IC₅₀	6.976 μM Compound: NMS-P715	Antiproliferative activity against human EKVX cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human EKVX cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
Granta-519	IC₅₀	1.687 μM Compound: NMS-P715	Antiproliferative activity against human Granta-519 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human Granta-519 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
HCC 2998	IC₅₀	1.813 μM Compound: NMS-P715	Antiproliferative activity against human HCC 2998 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human HCC 2998 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
HCC78	IC₅₀	10 μM Compound: NMS-P715	Antiproliferative activity against human HCC78 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human HCC78 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
HCT-116	GI₅₀	0.18 μM Compound: 5, NMS-P715	Growth inhibition in human HCT116 cells after 72 hrs by MTT assay Growth inhibition in human HCT116 cells after 72 hrs by MTT assay	[PMID: 24256217]
HCT-116	IC₅₀	0.16 μM Compound: 5, NMS-P715	Inhibition of Myc-tagged wild type MPS1 autophosphorylation in human HCT116 cells after 2 hrs in presence of proteosome inhibitor MG132 Inhibition of Myc-tagged wild type MPS1 autophosphorylation in human HCT116 cells after 2 hrs in presence of proteosome inhibitor MG132	[PMID: 24256217]
HCT-116	IC₅₀	153.8 nM Compound: Chemical probe: NMS-P715	Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability incubated for 5 days Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability incubated for 5 days	[PMID: 26364596]
HCT-116	IC₅₀	1625 nM Compound: Chemical probe: NMS-P715	Inhibition of colony formation in human HCT-116 cells harboring Mps1 I531M mutant assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay Inhibition of colony formation in human HCT-116 cells harboring Mps1 I531M mutant assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay	[PMID: 26364596]
HCT-116	IC₅₀	171 nM Compound: Chemical probe: NMS-P715	Inhibition of colony formation in human HCT-116 cells assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay Inhibition of colony formation in human HCT-116 cells assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay	[PMID: 26364596]
HCT-116	IC₅₀	2.036 μM Compound: NMS-P715	Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
HCT-116	IC₅₀	> 2000 nM Compound: Chemical probe: NMS-P715	Inhibition of colony formation in human HCT-116 cells harboring Mps1 C604Y mutant assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay Inhibition of colony formation in human HCT-116 cells harboring Mps1 C604Y mutant assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay	[PMID: 26364596]
HCT-15	IC₅₀	0.232 μM Compound: NMS-P715	Antiproliferative activity against human HCT-15 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human HCT-15 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
HEL	IC₅₀	5.486 μM Compound: NMS-P715	Antiproliferative activity against human HEL cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human HEL cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
HL-60	IC₅₀	1.584 μM Compound: NMS-P715	Antiproliferative activity against human HL-60 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human HL-60 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
HOP-62	IC₅₀	7.771 μM Compound: NMS-P715	Antiproliferative activity against human HOP-62 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human HOP-62 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
HOP-92	IC₅₀	2.178 μM Compound: NMS-P715	Antiproliferative activity against human HOP-92 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human HOP-92 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
HT-29	IC₅₀	3.918 μM Compound: NMS-P715	Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
HT1197	IC₅₀	8.304 μM Compound: NMS-P715	Antiproliferative activity against human HT1197 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human HT1197 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
HeLa	IC₅₀	1330 nM Compound: Chemical probe: NMS-P715	Inhibition of colony formation in human HeLa cells harboring Mps1 I598F mutant assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay Inhibition of colony formation in human HeLa cells harboring Mps1 I598F mutant assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay	[PMID: 26364596]
HeLa	IC₅₀	1720 nM Compound: Chemical probe: NMS-P715	Inhibition of colony formation in human HeLa cells harboring Mps1 C604F mutant assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay Inhibition of colony formation in human HeLa cells harboring Mps1 C604F mutant assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay	[PMID: 26364596]
HeLa	IC₅₀	433 nM Compound: Chemical probe: NMS-P715	Inhibition of colony formation in human HeLa cells assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay Inhibition of colony formation in human HeLa cells assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay	[PMID: 26364596]
HeLa	IC₅₀	6.46 μM Compound: NMS-P715	Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
HeLa	IC₅₀	> 2000 nM Compound: Chemical probe: NMS-P715	Inhibition of colony formation in human HeLa cells harboring Mps1 C604Y mutant assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay Inhibition of colony formation in human HeLa cells harboring Mps1 C604Y mutant assessed as cell survival incubated for 5 days by crystal violet staining based colony formation assay	[PMID: 26364596]
Hs-578T	IC₅₀	6.638 μM Compound: NMS-P715	Antiproliferative activity against human Hs-578T cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human Hs-578T cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
IGROV-1	IC₅₀	1.152 μM Compound: NMS-P715	Antiproliferative activity against human IGROV-1 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human IGROV-1 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
Jurkat	IC₅₀	3.606 μM Compound: NMS-P715	Antiproliferative activity against human Jurkat cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human Jurkat cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
K562	IC₅₀	2.705 μM Compound: NMS-P715	Antiproliferative activity against human K562 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human K562 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
KARPAS-299	IC₅₀	3.533 μM Compound: NMS-P715	Antiproliferative activity against human KARPAS-299 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human KARPAS-299 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
KG-1	IC₅₀	2.958 μM Compound: NMS-P715	Antiproliferative activity against human KG-1 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human KG-1 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
KM12	IC₅₀	0.586 μM Compound: NMS-P715	Antiproliferative activity against human KM12 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human KM12 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
L-363	IC₅₀	1.607 μM Compound: NMS-P715	Antiproliferative activity against human L-363 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human L-363 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
LNCaP	IC₅₀	3.072 μM Compound: NMS-P715	Antiproliferative activity against human LNCaP cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human LNCaP cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
LP-1	IC₅₀	3.479 μM Compound: NMS-P715	Antiproliferative activity against human LP-1 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human LP-1 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
LS174T	IC₅₀	1.936 μM Compound: NMS-P715	Antiproliferative activity against human LS174T cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human LS174T cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
LS180	IC₅₀	2.168 μM Compound: NMS-P715	Antiproliferative activity against human LS180 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human LS180 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
LoVo	IC₅₀	1.87 μM Compound: NMS-P715	Antiproliferative activity against human LoVo cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human LoVo cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
M14	IC₅₀	0.698 μM Compound: NMS-P715	Antiproliferative activity against human M14 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human M14 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
MCF7	IC₅₀	1.86 μM Compound: NMS-P715	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
MDA-MB-231	IC₅₀	0.899 μM Compound: NMS-P715	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
MDA-MB-435	IC₅₀	1.955 μM Compound: NMS-P715	Antiproliferative activity against human MDA-MB-435 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human MDA-MB-435 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
MIA PaCa-2	IC₅₀	2.549 μM Compound: NMS-P715	Antiproliferative activity against human MIA PaCa-2 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human MIA PaCa-2 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
MOLM-13	IC₅₀	1.041 μM Compound: NMS-P715	Antiproliferative activity against human MOLM-13 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human MOLM-13 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
MOLT-4	IC₅₀	1.041 μM Compound: NMS-P715	Antiproliferative activity against human MOLT-4 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human MOLT-4 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
MV4-11	IC₅₀	1.141 μM Compound: NMS-P715	Antiproliferative activity against human MV4-11 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human MV4-11 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
MeWo	IC₅₀	3.257 μM Compound: NMS-P715	Antiproliferative activity against human MeWo cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human MeWo cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
NCI-H146	IC₅₀	4.283 μM Compound: NMS-P715	Antiproliferative activity against human NCI-H146 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human NCI-H146 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
NCI-H1770	IC₅₀	4.415 μM Compound: NMS-P715	Antiproliferative activity against human NCI-H1770 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human NCI-H1770 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
NCI-H1975	IC₅₀	10 μM Compound: NMS-P715	Antiproliferative activity against human NCI-H1975 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human NCI-H1975 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
NCI-H2126	IC₅₀	4.632 μM Compound: NMS-P715	Antiproliferative activity against human NCI-H2126 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human NCI-H2126 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
NCI-H2228	IC₅₀	5.812 μM Compound: NMS-P715	Antiproliferative activity against human NCI-H2228 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human NCI-H2228 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
NCI-H226	IC₅₀	2.007 μM Compound: NMS-P715	Antiproliferative activity against human NCI-H226 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human NCI-H226 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
NCI-H23	IC₅₀	1.216 μM Compound: NMS-P715	Antiproliferative activity against human NCI-H23 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human NCI-H23 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
NCI-H322M	IC₅₀	1.555 μM Compound: NMS-P715	Antiproliferative activity against human NCI-H322M cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human NCI-H322M cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
NCI-H460	IC₅₀	2.191 μM Compound: NMS-P715	Antiproliferative activity against human NCI-H460 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human NCI-H460 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
NCI-H522	IC₅₀	1.205 μM Compound: NMS-P715	Antiproliferative activity against human NCI-H522 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human NCI-H522 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
NCI-H526	IC₅₀	2.203 μM Compound: NMS-P715	Antiproliferative activity against human NCI-H526 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human NCI-H526 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
NCI-H82	IC₅₀	2.59 μM Compound: NMS-P715	Antiproliferative activity against human NCI-H82 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human NCI-H82 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
NCI-H889	IC₅₀	1.26 μM Compound: NMS-P715	Antiproliferative activity against human NCI-H889 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human NCI-H889 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
NCI-H929	IC₅₀	10 μM Compound: NMS-P715	Antiproliferative activity against human NCI-H929 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human NCI-H929 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
NCI/ADR-RES	IC₅₀	2.484 μM Compound: NMS-P715	Antiproliferative activity against human NCI-ADR-RES cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human NCI-ADR-RES cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
NHDF	IC₅₀	10 μM Compound: NMS-P715	Antiproliferative activity against human NHDF cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human NHDF cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
OPM-2	IC₅₀	2.31 μM Compound: NMS-P715	Antiproliferative activity against human OPM-2 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human OPM-2 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
OVCAR-3	IC₅₀	6.202 μM Compound: NMS-P715	Antiproliferative activity against human OVCAR-3 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human OVCAR-3 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
OVCAR-8	IC₅₀	1.212 μM Compound: NMS-P715	Antiproliferative activity against human OVCAR-8 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human OVCAR-8 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
PC-3	IC₅₀	1.992 μM Compound: NMS-P715	Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
RERF-LC-AI	IC₅₀	2.068 μM Compound: NMS-P715	Antiproliferative activity against human RERF-LC-AI cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human RERF-LC-AI cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
RO82-W-1	IC₅₀	9.308 μM Compound: NMS-P715	Antiproliferative activity against human RO82-W-1 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human RO82-W-1 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
RPMI-8226	IC₅₀	1.484 μM Compound: NMS-P715	Antiproliferative activity against human RPMI-8226 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human RPMI-8226 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
SET-2	IC₅₀	10 μM Compound: NMS-P715	Antiproliferative activity against human SET2 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human SET2 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
SF-268	IC₅₀	1.372 μM Compound: NMS-P715	Antiproliferative activity against human SF-268 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human SF-268 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
SF-295	IC₅₀	2.881 μM Compound: NMS-P715	Antiproliferative activity against human SF-295 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human SF-295 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
SF-539	IC₅₀	0.807 μM Compound: NMS-P715	Antiproliferative activity against human SF-539 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human SF-539 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
SK-LU-1	IC₅₀	5.002 μM Compound: NMS-P715	Antiproliferative activity against human SK-LU-1 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human SK-LU-1 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
SK-MEL-2	IC₅₀	1.492 μM Compound: NMS-P715	Antiproliferative activity against human SK-MEL-2 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human SK-MEL-2 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
SK-MEL-28	IC₅₀	2.594 μM Compound: NMS-P715	Antiproliferative activity against human SK-MEL-28 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human SK-MEL-28 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
SK-MEL-5	IC₅₀	4.486 μM Compound: NMS-P715	Antiproliferative activity against human SK-MEL-5 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human SK-MEL-5 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
SK-OV-3	IC₅₀	4.258 μM Compound: NMS-P715	Antiproliferative activity against human SK-OV-3 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human SK-OV-3 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
SKM-1	IC₅₀	10 μM Compound: NMS-P715	Antiproliferative activity against human SKM-1 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human SKM-1 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
SN12C	IC₅₀	1.225 μM Compound: NMS-P715	Antiproliferative activity against human SN12C cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human SN12C cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
SNB-19	IC₅₀	6.928 μM Compound: NMS-P715	Antiproliferative activity against human SNB-19 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human SNB-19 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
SNB-75	IC₅₀	2.202 μM Compound: NMS-P715	Antiproliferative activity against human SNB-75 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human SNB-75 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
SR	IC₅₀	6.959 μM Compound: NMS-P715	Antiproliferative activity against human SR cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human SR cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
SU-DHL-1	IC₅₀	3.309 μM Compound: NMS-P715	Antiproliferative activity against human SU-DHL-1 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human SU-DHL-1 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
SU-DHL-6	IC₅₀	10 μM Compound: NMS-P715	Antiproliferative activity against human SU-DHL-6 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human SU-DHL-6 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
SU-DHL-8	IC₅₀	0.829 μM Compound: NMS-P715	Antiproliferative activity against human SU-DHL-8 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human SU-DHL-8 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
SU-DHL10	IC₅₀	1.579 μM Compound: NMS-P715	Antiproliferative activity against human SU-DHL-10 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human SU-DHL-10 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
SUD4	IC₅₀	1.605 μM Compound: NMS-P715	Antiproliferative activity against human SU-DHL-4 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human SU-DHL-4 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
SW-620	IC₅₀	0.472 μM Compound: NMS-P715	Antiproliferative activity against human SW620 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human SW620 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
SW1417	IC₅₀	8.842 μM Compound: NMS-P715	Antiproliferative activity against human SW1417 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human SW1417 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
SW48	IC₅₀	0.551 μM Compound: NMS-P715	Antiproliferative activity against human SW48 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human SW48 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
SW480	IC₅₀	1.298 μM Compound: NMS-P715	Antiproliferative activity against human SW480 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human SW480 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
SW948	IC₅₀	1.955 μM Compound: NMS-P715	Antiproliferative activity against human SW948 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human SW948 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
Sf9	IC₅₀	0.007 μM Compound: 5, NMS-P715	Inhibition of N-terminal 6XHis-tagged/GST-tagged full length human MPS1 expressed in recombinant baculovirus infected sf9 insect cells using 5FAM-DHTGFLTEYVATRCONH2 as substrate after 60 to 90 mins by fluorescence assay Inhibition of N-terminal 6XHis-tagged/GST-tagged full length human MPS1 expressed in recombinant baculovirus infected sf9 insect cells using 5FAM-DHTGFLTEYVATRCONH2 as substrate after 60 to 90 mins by fluorescence assay	[PMID: 24256217]
T47D	IC₅₀	2.864 μM Compound: NMS-P715	Antiproliferative activity against human T47D cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human T47D cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
T84	IC₅₀	10 μM Compound: NMS-P715	Antiproliferative activity against human T84 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human T84 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
T98G	IC₅₀	0.203 μM Compound: NMS-P715	Antiproliferative activity against human T98G cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human T98G cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
TF-1	IC₅₀	6.526 μM Compound: NMS-P715	Antiproliferative activity against human TF-1 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human TF-1 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
TK-10	IC₅₀	1.539 μM Compound: NMS-P715	Antiproliferative activity against human TK-10 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human TK-10 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
Toledo	IC₅₀	1.72 μM Compound: NMS-P715	Antiproliferative activity against human Toledo cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human Toledo cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
U-251	IC₅₀	1.821 μM Compound: NMS-P715	Antiproliferative activity against human U-251 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human U-251 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
U-266	IC₅₀	5.926 μM Compound: NMS-P715	Antiproliferative activity against human U-266 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human U-266 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
U2OS	IC₅₀	0.032 μM Compound: 15, NMS-P715	Inhibition of mitosis in human U2OS cells assessed as histone H3 phosphorylation at Ser 10 residue after 2 hrs by immunoassay Inhibition of mitosis in human U2OS cells assessed as histone H3 phosphorylation at Ser 10 residue after 2 hrs by immunoassay	[PMID: 21723120]
U2OS	IC₅₀	153 nM Compound: Chemical probe: NMS-P715	Antiproliferative activity against human U2OS cells assessed as reduction in cell viability incubated for 5 days Antiproliferative activity against human U2OS cells assessed as reduction in cell viability incubated for 5 days	[PMID: 26364596]
U2OS	IC₅₀	2.09 μM Compound: NMS-P715	Antiproliferative activity against human U2OS cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human U2OS cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
UACC-257	IC₅₀	1.866 μM Compound: NMS-P715	Antiproliferative activity against human UACC-257 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human UACC-257 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
UACC-62	IC₅₀	4.751 μM Compound: NMS-P715	Antiproliferative activity against human UACC-62 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human UACC-62 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]
UO-31	IC₅₀	3.616 μM Compound: NMS-P715	Antiproliferative activity against human UO-31 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human UO-31 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 21159646]

In Vitro

NMS-P715 is a selective inhibitor of MPS1, with an IC₅₀ of 182 nM. NMS-P715 is highly specific for MPS1, with no other kinases inhibited below an IC₅₀ value of 5 μM and only 3 kinases inhibited below 10 μM (CK2, MELK, and NEK6). NMS-P715 promotes massive spindle assembly checkpoint (SAC) override with an EC₅₀ of 65 nM. NMS-P715 (1 μM) causes mitotic acceleration in U2OS cells overexpressing YFP-α-tubulin, induces aneuploidy and inhibits the proliferation of HCT116 cells. NMS-P715 (0.5, 1 μM) affects mitotic checkpoint complex (MCC) stability and cdc20 ubiquitylation^[1]. NMS-P715 (1 μM) exhibits bypass of the spindle assembly checkpoint and apoptosis in pancreatic ductal adenocarcinoma (PDAC) cell lines. NMS-P715 (0-25 μM) also selectively inhibits growth of PDAC cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

NMS-P715 (10 mg/kg) exhibits an oral bioavailability of 37% and good pharmacokinetic properties in nude mice bearing subcutaneous implanted human tumor cell xenografts. NMS-P715 (90 mg/kg, p.o.) is well tolerated and cuases no signs of body weight loss or other overt toxicities in an A2780 ovary carcinoma xenograft model. NMS-P715 (100 mg/kg, p.o.) inhibits the tumor growth by appr 43% in the A375 melanoma xenograft model^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

676.73

Formula

C₃₅H₃₉F₃N₈O₃

CAS No.

1202055-32-0

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=NN(C)C2=C1CCC3=C2N=C(NC4=CC=C(C(NC5CCN(CC5)C)=O)C=C4OC(F)(F)F)N=C3)NC6=C(CC)C=CC=C6CC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 2 mg/mL (2.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4777 mL	7.3885 mL	14.7769 mL
5 mM	---	---	---
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.5% CMC/saline water
Solubility: 3.33 mg/mL (4.92 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

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(per animal)

μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.30%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (248 KB) HNMR (302 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Colombo R, et al. Targeting the mitotic checkpoint for cancer therapy with NMS-P715, an inhibitor of MPS1 kinase. Cancer Res. 2010 Dec 15;70(24):10255-64. [Content Brief]

[2]. Slee RB, et al. Selective inhibition of pancreatic ductal adenocarcinoma cell growth by the mitotic MPS1 kinase inhibitor NMS-P715. Mol Cancer Ther. 2014 Feb;13(2):307-315. [Content Brief]

Kinase Assay ^[1]	The potency of the compound towards MPS1 and 60 additional kinases belonging to kinase selectivity screening (KSS) panel is determined using either a strong anion exchanger based assay or P81 Multiscreen plate. MPS1 activity is measured using 5 nM of MPS1 recombinant protein in 50 mM HEPES pH 7.5, 2.5 mM MgCl₂, 1 mM MnCl₂, 1 mM DTT, 3 μM NaVO₃, 2 mM β-glycerophosphate, 0.2 mg/mL BSA, 200 μM P38-βtide substrate-peptide (KRQADEEMTGYVATRWYRAE) and 8 μM ATP with 1.5 nM ³³P-γ-ATP. The assay is run in a robotized format, 10 serial 1:3 compounds dilutions (including NMS-P715, from 30 μM to 1.5 nM) are tested and IC₅₀ determined^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Assay ^[1]	Cells lines are seeded in 384 well-plates in the appropiate complete medium and treated with compounds (NMS-P715, etc.) dissolved in 0.1% DMSO 24 hours after seeding. The cells are incubated at 37°C and 5% CO₂ and after 72 hours the plates are processed using CellTiter-Glo assay. Inhibitory activity is evaluated comparing treated versus control data using Assay Explorer software. IC₅₀ of proliferation is calculated using sigmoidal interpolation curve fitting. Activity Ratio is calculated as the ratio of the single cell line IC₅₀ and the IC₅₀ average of all the cell lines tested^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	Mice^[1] Athymic nu-nu mice, 5-6 weeks of age (20-22 g) are used in the assay. A2780 ovary carcinoma and A375 melanoma cells are transplanted s.c. into female nu-nu mice. Mice bearing a palpable tumor (100-200 mm³) are selected and randomized into control and treated groups. Treatment starts one day after randomization. NMS-P715 is typically administered by oral administration at doses of 90-100 mg/kg daily for more than seven days. Each group includs 8 animals. Tumor dimension is measured regularly by calipers during the experiments and tumor mass is calculated^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Colombo R, et al. Targeting the mitotic checkpoint for cancer therapy with NMS-P715, an inhibitor of MPS1 kinase. Cancer Res. 2010 Dec 15;70(24):10255-64. [Content Brief] [2]. Slee RB, et al. Selective inhibition of pancreatic ductal adenocarcinoma cell growth by the mitotic MPS1 kinase inhibitor NMS-P715. Mol Cancer Ther. 2014 Feb;13(2):307-315. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO

1 mM

1.4777 mL

7.3885 mL

14.7769 mL

36.9424 mL

NMS-P715 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NMS-P7151202055-32-0Mps1Monopolar spindle 1Inhibitorinhibitorinhibit

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