NS6180

Cat. No.: HY-15707 Purity: 99.80%: FAQs
Data Sheet SDS COA Handling Instructions

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NS6180 is a new orally active KCa3.1 channel inhibitor. NS6180 inhibits cloned human KCa3.1 channels with an IC₅₀value of 9 nM. NS6180 can be used for the research of inflammatory bowel disease (IBD) .

For research use only. We do not sell to patients.

NS6180 Chemical Structure

CAS No. : 353262-04-1

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 95	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 95	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 86	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 145	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 541	In-stock	Estimated Time of Arrival: December 31
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Biological Activity
Purity & Documentation
References
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Description

In Vitro

NS6180 (0.001-1 μM) shows inhibition of human KCa3.1 with an IC₅₀ value of 9.4 nM and a K₅₀ value of 11 1.7 nM, respectively^[1].
NS6180 (30 nM, 10 μM) has inhibition that dependent on amino acid residues T250 and V275^[1].
NS6180 (1, 10, 100 and 1000 nM; 1 min) shows CCCPreported hyperpolarizations of human erythrocytes^[1].
NS6180 (1, 10, 100 and 1000 nM; 1 min) blocks the erythrocyte KCa3.1 channels with IC₅₀ values of 14 nM (human KCa3.1 channels), 15 nM (mouse) and 9 nM (rats), respectively^[1]. NS6180 (0-5 μM, 48 h) suppresses rat and mouse splenocyte proliferation at submicrolar concentrations and potently inhibited IL-2 and IFN-g production, while exerting smaller effects on IL-4 and TNF-α and no effect on IL-17 production^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

NS6180 (i.v., i.p. and oral administration; 10 mg/kg; twice daily or once daily) has extremely low bioavailability and reduces DNBS-induced experimental colitis in rats^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Rats^[1]
Dosage:	10 mg/kg
Administration:	i.v., i.p. and oral administration; 10 mg/kg; twice daily or once daily
Result:	Had a plasma half-life of 3.8 h, oral or i.p. administration gave low plasma exposure (C_max: 186 nM and 33 nM, respectively, after administration of 10 mg/kg).

Molecular Weight

323.33

Formula

C₁₆H₁₂F₃NOS

CAS No.

353262-04-1

Appearance

Solid

Color

White to off-white

SMILES

O=C1CSC2=CC=CC=C2N1CC3=CC=CC(C(F)(F)F)=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (309.28 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0928 mL	15.4641 mL	30.9282 mL
5 mM	0.6186 mL	3.0928 mL	6.1856 mL
10 mM	0.3093 mL	1.5464 mL	3.0928 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.80%

Select Batch:

Data Sheet (277 KB) SDS (0 KB)

COA (253 KB) LCMS (96 KB)

Handling Instructions (2659 KB)

References

[1]. Strobak D, et al. NS6180, a new K(Ca) 3.1 channel inhibitor prevents T-cell activation and inflammation in a rat model of inflammatory bowel disease. Br J Pharmacol. 2013 Jan;168(2):432-44. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.0928 mL	15.4641 mL	30.9282 mL	77.3204 mL
	5 mM	0.6186 mL	3.0928 mL	6.1856 mL	15.4641 mL
	10 mM	0.3093 mL	1.5464 mL	3.0928 mL	7.7320 mL
	15 mM	0.2062 mL	1.0309 mL	2.0619 mL	5.1547 mL
	20 mM	0.1546 mL	0.7732 mL	1.5464 mL	3.8660 mL
	25 mM	0.1237 mL	0.6186 mL	1.2371 mL	3.0928 mL
	30 mM	0.1031 mL	0.5155 mL	1.0309 mL	2.5773 mL
	40 mM	0.0773 mL	0.3866 mL	0.7732 mL	1.9330 mL
	50 mM	0.0619 mL	0.3093 mL	0.6186 mL	1.5464 mL
	60 mM	0.0515 mL	0.2577 mL	0.5155 mL	1.2887 mL
	80 mM	0.0387 mL	0.1933 mL	0.3866 mL	0.9665 mL
	100 mM	0.0309 mL	0.1546 mL	0.3093 mL	0.7732 mL

NS6180 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NS6180353262-04-1NS 6180NS-6180Potassium ChannelKcsAKCa3.1 channelinflammatory bowel disease (IBD)ratspharmacokineticsInhibitorinhibitorinhibit

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