Propylthiouracil
Based on 3 publication(s) in Google Scholar
Propylthiouracil (6-n-Propylthiouracil), a thioamide antithyroid agent, is an orally active thyroperoxidase and type-1 deiodinase (DIO1) inhibitor. Propylthiouracil can be used for the Graves disease and hyperthyroidism research.
For research use only. We do not sell to patients.
- Purity: 99.40%
- CAS No.: 51-52-5
- Formula: C7H10N2OS
- Molecular Weight:170.23
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Propylthiouracil
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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In Vivo Efficacy Study
Biological Activity
Propylthiouracil (5.5-330 μg/mL; 24 h) induces growth retardation and cytotoxicity in a dose-dependent manner in U-937 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:U-937 cells
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Concentration:5.5 μg/mL, 11 μg/mL, 110 μg/mL, 220 μg/mL, 330 μg/mL
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Incubation Time:24 hours
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Result:Induced cytotoxicity in a dose-dependent manner.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Adult C57BL/6J and wild-derived WSB/EiJ male mice (8-weeks old)[1]
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Dosage:1.5 g/kg diet
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Administration:Iodine-deficient diet; for 7 weeks
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Result:Induced hypothyroidism in adult C57BL/6J and wild-derived WSB/EiJ male mice.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 51-52-5
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Appearance Solid
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Molecular Weight 170.23
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Formula C7H10N2OS
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Color White to off-white
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SMILES
O=C(C=C(CCC)N1)NC1=S
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Synonyms
6-n-Propylthiouracil; 6-Propyl-2-thiouracil; PTU
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (3)
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Journal Impact Factor
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Most Recent
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Commun Biol
Pantothenic acid ameliorates hepatic fibrosis by targeting IGFBP6 to regulate the TGF-β/SMADs pathway. [Abstract]2025 Jul 29;8(1):1127. PMID: 40730679 -
Toxicol Appl Pharmacol
Toxicokinetic insights into distinct mechanisms of action of two thyroid toxicants: Propylthiouracil and pregnenolone 16α‑carbonitrile. [Abstract]2025 May:498:117282. PMID: 40023229 -
Animal Model Exp Med
2024 Dec;7(6):926-935. PMID: 38946346
Propylthiouracil purchased from MedChemExpress. Usage Cited in: Animal Model Exp Med. 2024 Dec;7(6):926-935. [Abstract]
Propylthiouracil (PTU; 1 ppm; 9 weeks) induced Isolated maternal hypothyroxinemia in Wistar rats. Propylthiouracil showed that the concentration of T4 in the MC group (N = 5) was significantly lower than that of the T4 in NC group, the concentration of T3 and TSH did not change significantly.
Propylthiouracil purchased from MedChemExpress. Usage Cited in: Animal Model Exp Med. 2024 Dec;7(6):926-935. [Abstract]
Propylthiouracil (PTU; 1 ppm; 9 weeks) induced Isolated maternal hypothyroxinemia in Wistar rats. Propylthiouracil showed a significant decrease in the weight of the thyroid; the weight of the pituitary gland and the hypothalamus did not change significantly.
Propylthiouracil purchased from MedChemExpress. Usage Cited in: Animal Model Exp Med. 2024 Dec;7(6):926-935. [Abstract]
Propylthiouracil (PTU; 1 ppm; 9 weeks) showed that the number of marbles buried was significantly higher than that in the NC group.
Propylthiouracil purchased from MedChemExpress. Usage Cited in: Animal Model Exp Med. 2024 Dec;7(6):926-935. [Abstract]
Propylthiouracil (PTU; 1 ppm; 9 weeks) showed that the total distance of rats did not increase significantly compared with that in the NC group, the movement time of the MC group significantly increased and the immobility time significantly decreased.
Propylthiouracil purchased from MedChemExpress. Usage Cited in: Animal Model Exp Med. 2024 Dec;7(6):926-935. [Abstract]
Propylthiouracil (PTU; 1 ppm; 9 weeks) achieved the fastest learning speed on the third day.
Solvent & Solubility
DMSO : ≥ 100 mg/mL (587.44 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.69 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.69 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (418 KB)
- English - EN (418 KB)
- Français - FR (418 KB)
- Deutsch - DE (418 KB)
- Norwegian - NO (418 KB)
- Español - ES (418 KB)
- Swedish - SV (418 KB)
- Italian - IT (418 KB)
- Portuguese - PT (418 KB)
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Handling Instructions (2659 KB)
References
[1]. Lamis Chamas, et al. A Fine Regulation of the Hippocampal Thyroid Signalling Protects Hypothyroid Mice against Glial Cell Activation. Int J Mol Sci. 2022 Oct 8;23(19):11938. [Content Brief]
[2]. Utsana Puapermpoonsiri, et al. Synergistic effect of phospholipid-based liposomes and propylthiouracil on U-937 cell growth. J Liposome Res. 2005;15(3-4):215-27. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 5.8744 mL | 29.3720 mL | 58.7441 mL | 146.8601 mL |
| 5 mM | 1.1749 mL | 5.8744 mL | 11.7488 mL | 29.3720 mL | |
| 10 mM | 0.5874 mL | 2.9372 mL | 5.8744 mL | 14.6860 mL | |
| 15 mM | 0.3916 mL | 1.9581 mL | 3.9163 mL | 9.7907 mL | |
| 20 mM | 0.2937 mL | 1.4686 mL | 2.9372 mL | 7.3430 mL | |
| 25 mM | 0.2350 mL | 1.1749 mL | 2.3498 mL | 5.8744 mL | |
| 30 mM | 0.1958 mL | 0.9791 mL | 1.9581 mL | 4.8953 mL | |
| 40 mM | 0.1469 mL | 0.7343 mL | 1.4686 mL | 3.6715 mL | |
| 50 mM | 0.1175 mL | 0.5874 mL | 1.1749 mL | 2.9372 mL | |
| 60 mM | 0.0979 mL | 0.4895 mL | 0.9791 mL | 2.4477 mL | |
| 80 mM | 0.0734 mL | 0.3672 mL | 0.7343 mL | 1.8358 mL | |
| 100 mM | 0.0587 mL | 0.2937 mL | 0.5874 mL | 1.4686 mL |
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