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  4. Rhodamine 800

Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms.

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Rhodamine 800 Chemical Structure

Rhodamine 800 Chemical Structure

CAS No. : 137993-41-0

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Based on 1 publication(s) in Google Scholar

Rhodamine 800 Related Antibodies

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  • Biological Activity

  • Protocol

  • Purity & Documentation

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Description

Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms[1].

In Vitro

1.Preparation of Rhodamine 800 working solution
1.1Preparation of the stock solution
Dissolve 1 mg Rhodamine 800 in 525 μL DMSO to obtain 5 mM of stock solution.
1.2Preparation of Rhodamine 800 working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-20 μM of working solution.
Note: Please adjust the concentration of Rhodamine 800 working solution according to the actual situation.
2.Cell staining
2.1 Suspension cells (6-well plate)
a.Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.
b.Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c.Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d.Wash twice with PBS, 5 minutes each time.
e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a.Culture adherent cells on sterile coverslips.
b.Remove the coverslip from the medium and aspirate excess medium.
c.Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 30-60 minutes.
d.Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.
Note: If detection by flow cytometry, cells need to be resuspended before staining.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Rhodamine 800 Related Antibodies
Molecular Weight

495.95

Formula

C26H26ClN3O5
CAS No.
Appearance

Solid

Color

Dark purple to black

Emission (Em)

704
Excitation (Ex)

682
SMILES

N#CC(C1=CC2=C3N(CCC2)CCCC3=C1[O+]=C45)=C4C=C6CCCN7CCCC5=C76.O=Cl(=O)([O-])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 83.33 mg/mL (168.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0163 mL 10.0817 mL 20.1633 mL
5 mM 0.4033 mL 2.0163 mL 4.0327 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.72%

COA (252 KB) HNMR (272 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
References
Kinase Assay
[2]

Rhodamine 800 (Rh800) and indocyanine green (ICG) are dissolved in PBS and their concentrations are set to 40 nM. To 3 mL of Rhodamine 800 solution (40 nM/PBS), 100 μL of p-sulfonatocalix[n]arenes (S[n]) (1 mM) is added. To this solution, microliter aliquots of acetylcholine (ACh) (350 mM) are added under stirring. After 2 min stirring, fluorescence spectra are measured. For the competitive fluorophore displacement by other neurotransmitters, amino acids, ammonium chloride, and choline, similar procedure as the above method is applied[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0163 mL 10.0817 mL 20.1633 mL 50.4083 mL
5 mM 0.4033 mL 2.0163 mL 4.0327 mL 10.0817 mL
10 mM 0.2016 mL 1.0082 mL 2.0163 mL 5.0408 mL
15 mM 0.1344 mL 0.6721 mL 1.3442 mL 3.3606 mL
20 mM 0.1008 mL 0.5041 mL 1.0082 mL 2.5204 mL
25 mM 0.0807 mL 0.4033 mL 0.8065 mL 2.0163 mL
30 mM 0.0672 mL 0.3361 mL 0.6721 mL 1.6803 mL
40 mM 0.0504 mL 0.2520 mL 0.5041 mL 1.2602 mL
50 mM 0.0403 mL 0.2016 mL 0.4033 mL 1.0082 mL
60 mM 0.0336 mL 0.1680 mL 0.3361 mL 0.8401 mL
80 mM 0.0252 mL 0.1260 mL 0.2520 mL 0.6301 mL
100 mM 0.0202 mL 0.1008 mL 0.2016 mL 0.5041 mL
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Rhodamine 800 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Rhodamine 800137993-41-0Rhodamine800Rhodamine-800Fluorescent DyeInhibitorinhibitorinhibit

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