1. Dye Reagents
  2. Rhodamine 800

Rhodamine 800 

Cat. No.: HY-101876
Handling Instructions

Rhodamine 800 is a near-infrared fluorescent dye.

For research use only. We do not sell to patients.

Rhodamine 800 Chemical Structure

Rhodamine 800 Chemical Structure

CAS No. : 137993-41-0

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Rhodamine 800 is a near-infrared fluorescent dye.

In Vitro

Rhodamine 800 has different optical absorption spectra in various organic solvents and in aqueous solution in the visible and near-infrared regions. The absorption maximum at 683 nm in acetone shifts to 696 nm in DMSO without a significant change of the overall spectral profile. The fluorescence intensity of Rhodamine 800 varies markedly with the solvent, and decreases to less than one-fifth when the solvent is changed from acetone to water without a significant change of the emission peak[1].


Please store the product under the recommended conditions in the Certificate of Analysis.

Kinase Assay

Rhodamine 800 (Rh800) and indocyanine green (ICG) are dissolved in PBS and their concentrations are set to 40 nM. To 3 mL of Rhodamine 800 solution (40 nM/PBS), 100 μL of p-sulfonatocalix[n]arenes (S[n]) (1 mM) is added. To this solution, microliter aliquots of acetylcholine (ACh) (350 mM) are added under stirring. After 2 min stirring, fluorescence spectra are measured. For the competitive fluorophore displacement by other neurotransmitters, amino acids, ammonium chloride, and choline, similar procedure as the above method is applied[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight









Room temperature in continental US; may vary elsewhere

  • Molarity Calculator

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
Rhodamine 800
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Rhodamine 800

Cat. No.: HY-101876