Antibiofilm agent-22
Antibiofilm agent-22 is an FpvA receptor-targeting antibacterial agent. Antibiofilm agent-22 chelates iron, disrupts las, pqs, and rhl pathways, reduces elastase, pyocyanin, and rhamnolipid production, and induces bacterial iron starvation. Antibiofilm agent-22 can be used for the research of Pseudomonas aeruginosa infection.
For research use only. We do not sell to patients.
- Formula: C22H38N2O5
- Molecular Weight:410.55
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Antibiofilm agent-22 (Compound 5j) potently inhibits Pseudomonas aeruginosa PAO1 biofilm formation without direct bactericidal activity, with an IC50 of 2.66 μM[1].
Antibiofilm agent-22 (1.25-20 μM; overnight) inhibits biofilm development in Pseudomonas aeruginosa PAO1 in a concentration-dependent manner[1].
Antibiofilm agent-22 (1.25-20 μM) significantly and concentration-dependently reduces production of elastase, pyocyanin, and rhamnolipids in Pseudomonas aeruginosa PAO1, with maximum inhibition at 20 μM[1].
Antibiofilm agent-22 (1.25-20 μM; 24 h) inhibits the las, pqs, and rhl quorum sensing systems in Pseudomonas aeruginosa PAO1 without affecting GFP protein expression or bacterial growth[1].
Antibiofilm agent-22 (1.25-20 μM) acts as a potent iron chelator (pFe(III) = 22.99) that concentration-dependently enhances pyochelin and pyoverdine production in Pseudomonas aeruginosa PAO1 by inducing iron starvation[1].
Antibiofilm agent-22 (24 h) disrupts iron homeostasis and impairs processes critical to biofilm formation (including pilus assembly) in Pseudomonas aeruginosa PAO1 via transcriptomic dysregulation[1].
Antibiofilm agent-22 and its Fe3+ dimer exhibit strong, stable binding affinity to the Pseudomonas aeruginosa FpvA receptor, competing with native pyoverdine to disrupt iron acquisition[1].
Antibiofilm agent-22 (1.25-20 μM) inhibits swarming motility in Pseudomonas aeruginosa PAO1 in a concentration-dependent manner (78% inhibition at 20 μM) and induces cytoplasmic vacuolization and membrane thinning without direct bactericidal activity[1].
Antibiofilm agent-22 acts synergistically with Tobramycin (HY-B0441) against Pseudomonas aeruginosa PAO1, reducing the antibiotic's MIC by 32-fold (FICI = 0.078)[1].
Antibiofilm agent-22 (3.125-50 μM) exhibits no significant hemolytic activity against rabbit erythrocytes at concentrations up to 50 μM[1].
Antibiofilm agent-22 (6.25-100 μM; 24 h) shows no significant cytotoxicity to RAW 264.7, HSC, or 293T cells at concentrations up to 100 μM after 24-hour incubation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:RAW 264.7 macrophages, human hepatic stellate cells (HSCs), human embryonic kidney 293T cells
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Concentration:6.25 μM; 12.5 μM; 25 μM; 50 μM; 100 μM
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Incubation Time:24 h
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Result:Did not exhibit significant cytotoxicity to any of the three cell types at concentrations up to 100 μM.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:ICR (male, 3-4 weeks old, P. aeruginosa single-species
infection model)[1] -
Dosage:25 μM; 25 μM plus Tobramycin sulfate
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Administration:topical; daily for 3 or 9 days
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Result:Did not adversely affect mouse body weight, indicating favorable biosafety at the tested concentration.
Showed no statistically significant difference in wound bacterial load compared to the infection control group.
Achieved near-complete bacterial clearance from the wounds.
Chemical Information
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Molecular Weight 410.55
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Formula C22H38N2O5
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SMILES
O=C1C=C(COCCCCCCC(NO)=O)N(CCCCCCCCC)C=C1O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Antibiofilm agent-22 Related Classifications
HY-183361 Related Classifications
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