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PGC1 alpha Antibody

(Synonyms: PPARGC1A; LEM6; PGC1; PGC1A; PPARGC1; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; PGC-1-alpha; PPAR-gamma coactivator 1-alpha; PPARGC-1-alpha; Ligand effect modulator 6)
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Based on 1 publication(s) in Google Scholar

PGC1 alpha Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to PGC1 alpha.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PGC1 alpha Antibody
  • PGC1 alpha Antibody Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

PGC1 alpha Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to PGC1 alpha.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 113 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 91 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human PGC1 alpha aa765-798.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102709

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PGC1 alpha Antibody
    Western blot analysis of extracts from C6(lane 2(20μg) , C6(lane 3(40ug) and NIH/3T3(lane 4(20ug) , NIH/3T3(lane 5(40ug) using PGC1 alpha Antibody (HY-P80783) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Transcriptional coactivator for steroid receptors and nuclear receptors (PubMed:10713165, PubMed:20005308, PubMed:21376232, PubMed:28363985, PubMed:32433991). Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter (PubMed:10713165, PubMed:20005308, PubMed:21376232). Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis (PubMed:10713165, PubMed:20005308, PubMed:21376232). Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism (PubMed:10713165, PubMed:20005308, PubMed:21376232). Acts as a key regulator of gluconeogenesis: stimulates hepatic gluconeogenesis by increasing the expression of gluconeogenic enzymes, and acting together with FOXO1 to promote the fasting gluconeogenic program (PubMed:16753578, PubMed:23142079). Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner (PubMed:23836911). Also involved in the integration of the circadian rhythms and energy metabolism (By similarity). Required for oscillatory expression of clock genes, such as BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK (By similarity)

  • Subcellular Localization

    Nucleus; Nucleus, PML body; Nucleus; Cytoplasm; Nucleus; Nucleus; Nucleus, PML body; Nucleus

  • Expression


    Tissue_specificity:It is expressed in the heart, skeletal muscle, liver, and kidneys. Expression levels are low in the brain and pancreas, and extremely low in the intestine and white adipose tissue. In skeletal muscle, expression levels are lower in obese individuals than in lean individuals, and fasting can double the expression level in the skeletal muscle of obese individuals.

    Induction:Transcription is repressed by ZNF746 which binds to 'insulin response sequences' its promoter

  • Isoforms & Post-Translational Modification

    Q9UBK2 has 9 isomers: Q9UBK2-1: 91027 Da (predicted); Q9UBK2-2: 30031 Da (predicted); Q9UBK2-3: 91713 Da (predicted); Q9UBK2-4: 89641 Da (predicted); Q9UBK2-5: 31772 Da (predicted); Q9UBK2-6: 30717 Da (predicted); Q9UBK2-7: 14964 Da (predicted); Q9UBK2-8: 33157 Da (predicted); Q9UBK2-9: 77139 Da (predicted).
    Phosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2;Heavily acetylated by KAT2A/GCN5 under conditions of high nutrients, leading to inactivation of PPARGC1A (PubMed:16753578, PubMed:20005308, PubMed:23142079). Deacetylated by SIRT1 in low nutrients/high NAD conditions, leading to its activation (PubMed:20005308);Ubiquitinated. Ubiquitination by RNF34 induces proteasomal degradation

  • Subunit

    Homooligomer (PubMed:10713165). Interacts with MYBBP1A; inhibits MYBBP1A transcriptional activation (By similarity). Interacts with PRDM16, LPIN1 and PML (By similarity). Interacts (via LXXLL motif) with RORA and RORC (via AF-2 motif); activates RORA and RORC transcriptional activation (By similarity). Interacts with LRPPRC (PubMed:17050673). Interacts with FOXO1 (By similarity). Interacts with NR5A2 (PubMed:28363985, PubMed:32433991)

  • SwissProt ID

    Q9UBK2

  • Gene ID
    10891 [NCBI]
  • Synonyms

    PPARGC1A; LEM6; PGC1; PGC1A; PPARGC1; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; PGC-1-alpha; PPAR-gamma coactivator 1-alpha; PPARGC-1-alpha; Ligand effect modulator 6

  • Research Field

    Epigenetics and Nuclear Signaling

PGC1 alpha Antibody Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from C6(lane 2(20μg) , C6(lane 3(40ug) and NIH/3T3(lane 4(20ug) , NIH/3T3(lane 5(40ug) using PGC1 alpha Antibody (HY-P80783) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PGC1 alpha Antibody Product Image
    1/16
  • Experimental Validation Results for PGC1 alpha Antibody
  • PGC1 alpha Antibody Product Image
  • Experimental Validation Results for PGC1 alpha Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from C6(lane 2(20μg) , C6(lane 3(40ug) and NIH/3T3(lane 4(20ug) , NIH/3T3(lane 5(40ug) using PGC1 alpha Antibody (HY-P80783) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PGC1 alpha Antibody
  • PGC1 alpha Antibody Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from C6(lane 2(20μg) , C6(lane 3(40ug) and NIH/3T3(lane 4(20ug) , NIH/3T3(lane 5(40ug) using PGC1 alpha Antibody (HY-P80783) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PGC1 alpha Antibody Product Image WB