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AMPK beta 2 Antibody (YA3545)

(Synonyms: 5'-AMP-activated protein kinase subunit beta-2, AMPK subunit beta-2, PRKAB2)
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Based on 1 Customer Validation

AMPK beta 2 Antibody (YA3545) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to AMPK beta 2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for AMPK beta 2 Antibody (YA3545)
  • AMPK beta 2 Antibody (YA3545) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

AMPK beta 2 Antibody (YA3545) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to AMPK beta 2.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 30 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 30.3 kDa
Immunogen

Purified recombinant fragment of human PRKAB2 (AA: 1-120) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718781

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for AMPK beta 2 Antibody (YA3545)
    Immunofluorescence analysis of GC-7901 cells labeling PRKAB2 antibody (HY-P83848) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

Background

  • Function

    AMPK beta 2 is a Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3)

  • Isoforms & Post-Translational Modification

    O43741 has 2 isomers: O43741-1: 30302 Da (predicted); O43741-2: 21485 Da (predicted).
    Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK

  • Subunit

    AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3)

  • SwissProt ID

    O43741

  • Gene ID
    5565 [NCBI]
  • Synonyms

    5'-AMP-activated protein kinase subunit beta-2, AMPK subunit beta-2, PRKAB2

AMPK beta 2 Antibody (YA3545) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunofluorescence analysis of GC-7901 cells labeling PRKAB2 antibody (HY-P83848) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

  • AMPK beta 2 Antibody (YA3545) Product Image
    1/16
  • Experimental Validation Results for AMPK beta 2 Antibody (YA3545)
  • AMPK beta 2 Antibody (YA3545) Product Image
  • Experimental Validation Results for AMPK beta 2 Antibody (YA3545)
    1/1
    MedChemExpress Validation
     Immunofluorescence analysis of GC-7901 cells labeling PRKAB2 antibody (HY-P83848) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature
  • Experimental Validation Results for AMPK beta 2 Antibody (YA3545)
  • AMPK beta 2 Antibody (YA3545) Product Image
    MedChemExpress Validation

    Immunofluorescence analysis of GC-7901 cells labeling PRKAB2 antibody (HY-P83848) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

  • AMPK beta 2 Antibody (YA3545) Product Image ICC