ZBED1 (DREF) Antibody (YA8557)

Art. -Nr.: HY-P88873: Data Sheet User Guide for Antibodies
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ZBED1 (DREF) Antibody (YA8557) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to ZBED1 (DREF).

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Beschreibung
Verweise

Beschreibung

ZBED1 (DREF) Antibody (YA8557) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to ZBED1 (DREF).

Host

Mouse

Clonality

Monoclonal

Molekulargewicht

Predicted band size: 78 kDa

Species Reactivity

Human

SwissProt ID

O96006

Immunogen

Full length human recombinant protein of human ZBED1 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500
FC FC: Flow Cytometry	1:100

Sensitivity	Endogenous	Reinheit	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2a

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background

Function：Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase which sumoylates CHD3/Mi2-alpha, causing its release from DNA (PubMed:27068747). This results in suppression of CHD3/Mi2-alpha transcription repression, increased recruitment of RNA polymerase II to gene promoters and positive regulation of transcription including H1-5 and ribosomal proteins such as: RPS6, RPL10A, and RPL12 (PubMed:12663651, PubMed:17209048, PubMed:17220279, PubMed:27068747). The resulting increased transcriptional activity drives cell proliferation (PubMed:12663651, PubMed:17220279). Binds to 5'-TGTCG[CT]GA[CT]A-3' consensus sequences in gene promoters of ribosomal proteins (PubMed:12663651, PubMed:17209048, PubMed:17220279, PubMed:27068747)|(Microbial infection) Binds to human adenovirus gene promoters and contributes to transcriptional repression and virus growth inhibition during early stages of infection

Subcellular Localization：Nucleus, PML body,Nucleus,Nucleus, PML body

Expression：
Tissue_Specificity: Ubiquitously expressed at low levels (PubMed:9887332). Expression is highest in skeletal muscle, heart, spleen and placenta (PubMed:9887332)
Induction: Expression is linked to the cell cycle: low in serum-starved fibroblasts, increasing during the G1/S phase, highest during the S/G2 phase and then decreasing again

Isoforms & Post-Translational Modification：O96006: 694 amino acids, molecular weight 78156 Da.

Subunit：Homodimer and homomultimer (PubMed:17209048)

Synonyms

ALTE; DREF; hDREF; TRAMP

Dokumentation

User Guide for Antibodies (1077 KB)

Verweise

[1]. /biology-dictionary/nci-h358-cell.html [Content Brief]