Art. -Nr.: HY-146052

Autophagy inducer 3

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Autophagy inducer 3 has autophagy induced activity. Autophagy inducer 3 possesses robust autophagic cell death in diverse cancer cells sparing normal counterpart. Autophagy inducer 3 induces lethal autophagy by formation of characteristic autophagic vacuoles, LC3 puncta formation, upregulation of signature autophagy markers like Beclin and Atg family proteins.

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Reinheit: 98.02%
CAS. Nr.: 2691054-63-2
Formel: C₂₄H₄₃NO₂
Molecular Weight:377.60

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Autophagy inducer 3 Chemical Structure

CAS. Nr.: 2691054-63-2

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (1)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

Autophagy^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
CCD-18Co	IC₅₀	>10 μM Compound: 26b	Antiproliferative activity against human CCD-18Co cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay Antiproliferative activity against human CCD-18Co cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay	[PMID: 31926468]
COLO 205	IC₅₀	2.03 μM Compound: 26b	Antiproliferative activity against human COLO 205 cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay Antiproliferative activity against human COLO 205 cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay	[PMID: 31926468]
DLD-1	IC₅₀	4.46 μM Compound: 26b	Antiproliferative activity against human DLD-1 cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay Antiproliferative activity against human DLD-1 cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay	[PMID: 31926468]
HT-29	IC₅₀	4.15 μM Compound: 26b	Antiproliferative activity against human HT-29 cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay Antiproliferative activity against human HT-29 cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay	[PMID: 31926468]
LoVo	IC₅₀	3.33 μM Compound: 26b	Antiproliferative activity against human LoVo cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay Antiproliferative activity against human LoVo cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay	[PMID: 31926468]
SW48	IC₅₀	3.14 μM Compound: 26b	Antiproliferative activity against human SW48 cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay Antiproliferative activity against human SW48 cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay	[PMID: 31926468]
SW-620	IC₅₀	1.86 μM Compound: 26b	Antiproliferative activity against human SW-620 cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay Antiproliferative activity against human SW-620 cells assessed as reduction in cell growth incubated for 48 hrs by SRB assay	[PMID: 31926468]

In Vitro

Autophagy inducer 3 (compound 26b) (10 μM; 48 hours) effectively inhibits cell growth in diverse breast, lung and colon cancer cell lines^[1].
Autophagy inducer 3 (0-10 μM; 48 hours) exhibits potent antiproliferative activity in COLO-205, LOVO, HT-29, DLD-1, SW48 and SW-620 with IC₅₀s of 2.03 μM, 3.33 μM, 4.15 μM, 4.46 μM, 3.14 μM, 1.86 μM, respectively; and shows low cytotoxicity in normal human colon fibroblast CCD-18Co with IC₅₀ over 10 μM^[1].
Autophagy inducer 3 (7.5 μM; 18 hours) promotes non apoptotic cell death in DLD-1 cells by cellular granularity through vacuole formation and increase Annexin-V positive cells as well as PARP cleavage; and robustly induces the expression of classical autophagy markers like Beclin-1, Atg3, Atg5 and Atg7^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay

Cell Line:

MCF-7, MDA-MB-231, DLD-1, HT-29, A549 and NCI-H358^[1]
Concentration:

10 μM
Incubation Time:

48 hours
Result:

Effectively inhibited cell growth at 10 μM dose in diverse cancer cell lines with the inhibition rates of 89.28~97.66%.

Cell Autophagy Assay

Cell Line:

DLD-1^[1]
Concentration:

7.5 μM
Incubation Time:

18 hours
Result:

Promoted intracytoplasmic vacuole accumulation in colon cancer (DLD-1) cells; and robustly induced the expression of classical autophagy markers like Beclin-1, Atg3, Atg5 and Atg7.

Chemical Information

CAS. Nr. 2691054-63-2
Appearance Solid
Molecular Weight 377.60
Formel C₂₄H₄₃NO₂
Color White to off-white
SMILES

CCCCCCCCCCCCCC[C@H](O)[C@@H](N)CC1=CC=C(C=C1)OC
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

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Journal Impact Factor

bioRxiv

2025 Nov 3.

Lösungsmittel & Löslichkeit

In Vitro:

Ethanol : 100 mg/mL (264.83 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6483 mL

13.2415 mL

26.4830 mL

5 mM

0.5297 mL

2.6483 mL

5.2966 mL

10 mM

0.2648 mL

1.3242 mL

2.6483 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline

Solubility: 2.5 mg/mL (6.62 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.

Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% EtOH 90% Corn Oil

Solubility: 2.5 mg/mL (6.62 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (268 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Ganesher A, et al. New Spisulosine Derivative promotes robust autophagic response to cancer cells. Eur J Med Chem. 2020;188:112011. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
Ethanol	1 mM	2.6483 mL	13.2415 mL	26.4831 mL	66.2076 mL
	5 mM	0.5297 mL	2.6483 mL	5.2966 mL	13.2415 mL
	10 mM	0.2648 mL	1.3242 mL	2.6483 mL	6.6208 mL
	15 mM	0.1766 mL	0.8828 mL	1.7655 mL	4.4138 mL
	20 mM	0.1324 mL	0.6621 mL	1.3242 mL	3.3104 mL
	25 mM	0.1059 mL	0.5297 mL	1.0593 mL	2.6483 mL
	30 mM	0.0883 mL	0.4414 mL	0.8828 mL	2.2069 mL
	40 mM	0.0662 mL	0.3310 mL	0.6621 mL	1.6552 mL
	50 mM	0.0530 mL	0.2648 mL	0.5297 mL	1.3242 mL
	60 mM	0.0441 mL	0.2207 mL	0.4414 mL	1.1035 mL
	80 mM	0.0331 mL	0.1655 mL	0.3310 mL	0.8276 mL
	100 mM	0.0265 mL	0.1324 mL	0.2648 mL	0.6621 mL

Keywords

Autophagy inducer 3

1 Cited Publications

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Autophagy inducer 3

Anti-Cancer Compound Library

Autophagy Compound Library

Cell Death Library

Mitophagy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) Autophagy inducer 3

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Autophagy inducer 3

Anti-Cancer Compound Library

Autophagy Compound Library

Cell Death Library

Mitophagy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) Autophagy inducer 3

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Autophagy inducer 3 Related Classifications

HY-146052 Related Classifications