ATOX1-IN-1
Based on 1 Customer Validation
ATOX1-IN-1 is an inhibitor of ATOX1 (a copper chaperone protein) with a Kd value of 12.5 μM. ATOX1-IN-1 induces intracellular copper accumulation, increases the level of DNA methylation in the NOTCH1 promoter region, and inhibits the NOTCH1/HES1 signaling pathway. ATOX1-IN-1 enhances the sensitivity of hepatocellular carcinoma cells to Cisplatin (HY-17394). ATOX1-IN-1 can be used in hepatocellular carcinoma-related research.
For research use only. We do not sell to patients.
- Purity : 99.71%
- CAS No.: 956181-19-4
- Formula: C33H26N2O6
- Molecular Weight:546.57
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
ATOX1-IN-1 (Compound 8) (48 h) inhibits the viability of HepG2 and Huh7 hepatocellular carcinoma cells with IC50 values of 16.5 μM and 22.0 μM, respectively, and exhibits high selectivity for cancer cells over immortalized hepatocytes THLE-2[1].
ATOX1-IN-1 (10 μM) with 10 μM Cisplatin exerts more significant antiproliferative and proapoptotic effects on HepG2 and Huh7 hepatocellular carcinoma cells[1].
ATOX1-IN-1 (5-15 μM) inhibits the NOTCH1/HES1 signaling pathway in HepG2 and Huh7 hepatocellular carcinoma cells in a concentration-dependent manner in vitro, and reduces the mRNA and protein expression levels of NOTCH1 and HES1[1].
ATOX1-IN-1 (5-15 μM) increases intracellular copper levels in HepG2 and Huh7 hepatocellular carcinoma cells in a concentration-dependent manner[1].
ATOX1-IN-1 (5-15 μM) enhances DNA methylation of the NOTCH1 promoter in HepG2 and Huh7 hepatocellular carcinoma cells in a concentration-dependent manner in vitro[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c nude (6-8 weeks old, 18-22 g, equal male/female ratio, subcutaneous HepG2 xenograft model)[1]
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Dosage:10 mg/kg
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Administration:i.p.; once daily for 10 days
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Result:Reduced tumor volume and proliferation marker (Ki67, PCNA) expression compared to the control group.
Produced a significantly greater tumor inhibition effect when combined with cisplatin than either single agent, with the combination group showing the smallest tumor volume, lowest Ki67 and PCNA expression, and no obvious pathological changes in major organs (heart, liver, lung, kidney, gastrointestinal tract).
Chemical Information
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CAS No. 956181-19-4
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Appearance Solid
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Molecular Weight 546.57
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Formula C33H26N2O6
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Color White to off-white
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SMILES
O=C(N[C@@H](CC1=CNC2=C1C=CC=C2)C(OC3=CC=C(C(C=CC=C4)=C4C(O5)=O)C5=C3C)=O)OCC6=CC=CC=C6
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (182.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.57 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8296 mL | 9.1480 mL | 18.2959 mL | 45.7398 mL |
| 5 mM | 0.3659 mL | 1.8296 mL | 3.6592 mL | 9.1480 mL | |
| 10 mM | 0.1830 mL | 0.9148 mL | 1.8296 mL | 4.5740 mL | |
| 15 mM | 0.1220 mL | 0.6099 mL | 1.2197 mL | 3.0493 mL | |
| 20 mM | 0.0915 mL | 0.4574 mL | 0.9148 mL | 2.2870 mL | |
| 25 mM | 0.0732 mL | 0.3659 mL | 0.7318 mL | 1.8296 mL | |
| 30 mM | 0.0610 mL | 0.3049 mL | 0.6099 mL | 1.5247 mL | |
| 40 mM | 0.0457 mL | 0.2287 mL | 0.4574 mL | 1.1435 mL | |
| 50 mM | 0.0366 mL | 0.1830 mL | 0.3659 mL | 0.9148 mL | |
| 60 mM | 0.0305 mL | 0.1525 mL | 0.3049 mL | 0.7623 mL | |
| 80 mM | 0.0229 mL | 0.1143 mL | 0.2287 mL | 0.5717 mL | |
| 100 mM | 0.0183 mL | 0.0915 mL | 0.1830 mL | 0.4574 mL |
ATOX1-IN-1 Related Classifications
HY-181975 Related Classifications
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