Atpenin A5
Based on 9 publication(s) in Google Scholar
Atpenin A5 is a potent and highly specific complex II inhibitor (IC50 ~10 nM), and is an effective mKATP channel agonist and cardioprotective agent.
For research use only. We do not sell to patients.
- Purity: 99.94%
- CAS No.: 119509-24-9
- Formula: C15H21Cl2NO5
- Molecular Weight:366.24
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Atpenin A5
More- Nat Metab. 2026 Jun;8(6):1390-1409. [Abstract]
- Biomed Pharmacother. 2024 Nov:180:117492. [Abstract]
- Anal Chem. 2025 Jun 3;97(21):11099-11109. [Abstract]
- Chin Med. 2024 May 13;19(1):68. [Abstract]
- Commun Biol. 2025 May 23;8(1):788. [Abstract]
- bioRxiv. 2025 Feb 19:2025.02.18.638948. [Abstract]
- Res Sq. 2025 Feb 16.
- University of Tennessee. 2025.
- bioRxiv. 2024 Jan 15.
Biological Activity
Atpenin A5 shows the inhibition profile for submitochondrial particles (SMPs), mitochondria, and cardiomyocytes, with IC50 values of 8.3, 9.3, and 8.5 nM, respectively. Atpenin A5 (AA5) is a potent and specific complex II inhibitor. Atpenin A5 (1 nM) also activates the mKATP channel and protects against simulated ischemia-reperfusion (IR) injury in isolated cardiomyocytes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Neonatal mice[2]
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Dosage:100 μg/kg
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Administration:Injected daily
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Result:Demonstrated myocardial thickness at the infarct zone and a significant reduction in scar size compared with controls.
Chemical Information
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CAS No. 119509-24-9
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Appearance Solid
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Molecular Weight 366.24
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Formula C15H21Cl2NO5
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Color White to off-white
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SMILES
O=C1C(C([C@@H](C)C[C@H](C)[C@@H](Cl)CCl)=O)=C(O)C(OC)=C(OC)N1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (9)
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Journal Impact Factor
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Most Recent
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Nat Metab
Succinate dehydrogenase loss suppresses pyrimidine biosynthesis via succinate-mediated inhibition of aspartate transcarbamylase. [Abstract]2026 Jun;8(6):1390-1409. PMID: 42082831 -
Biomed Pharmacother
Effect of statins on mitochondrial function and contractile force in human skeletal and cardiac muscle. [Abstract]2024 Nov:180:117492. PMID: 39326098 -
Anal Chem
Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]2025 Jun 3;97(21):11099-11109. PMID: 40401576 -
Chin Med
Acacetin alleviates autoimmune myocarditis by regulating CD4+ T cell mitochondrial respiration. [Abstract]2024 May 13;19(1):68. PMID: 38741130 -
Commun Biol
Nanoparticle-mediated targeting of PGC-1α reveals critical metabolic pathways in bladder cancer metastasis. [Abstract]2025 May 23;8(1):788. PMID: 40404885 -
bioRxiv
Succinate Dehydrogenase loss causes cascading metabolic effects that impair pyrimidine biosynthesis. [Abstract]2025 Feb 19:2025.02.18.638948. PMID: 40027747 -
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Solvent & Solubility
DMSO : 100 mg/mL (273.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.83 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.83 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Handling Instructions (2659 KB)
References
[1]. Andrew P Wojtovich, et al. The complex II inhibitor atpenin A5 protects against cardiac ischemia-reperfusion injury via activation of mitochondrial KATP channels. Basic Res Cardiol. 2009 Mar;104(2):121-9. [Content Brief]
[2]. Jiyoung Bae, et al. Malonate Promotes Adult Cardiomyocyte Proliferation and Heart Regeneration. Circulation. 2021 May 18;143(20):1973-1986. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7305 mL | 13.6523 mL | 27.3045 mL | 68.2613 mL |
| 5 mM | 0.5461 mL | 2.7305 mL | 5.4609 mL | 13.6523 mL | |
| 10 mM | 0.2730 mL | 1.3652 mL | 2.7305 mL | 6.8261 mL | |
| 15 mM | 0.1820 mL | 0.9102 mL | 1.8203 mL | 4.5508 mL | |
| 20 mM | 0.1365 mL | 0.6826 mL | 1.3652 mL | 3.4131 mL | |
| 25 mM | 0.1092 mL | 0.5461 mL | 1.0922 mL | 2.7305 mL | |
| 30 mM | 0.0910 mL | 0.4551 mL | 0.9102 mL | 2.2754 mL | |
| 40 mM | 0.0683 mL | 0.3413 mL | 0.6826 mL | 1.7065 mL | |
| 50 mM | 0.0546 mL | 0.2730 mL | 0.5461 mL | 1.3652 mL | |
| 60 mM | 0.0455 mL | 0.2275 mL | 0.4551 mL | 1.1377 mL | |
| 80 mM | 0.0341 mL | 0.1707 mL | 0.3413 mL | 0.8533 mL | |
| 100 mM | 0.0273 mL | 0.1365 mL | 0.2730 mL | 0.6826 mL |
Atpenin A5 Related Classifications
HY-126653 Related Classifications
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