2. Others Metabolic Enzyme/Protease
  3. Tyrosinase Environmental Pollutants
  4. Benzylacetone

Benzylacetone  (Synonyms: 4-Penylbutan-2-one)

Cat. No.: HY-W015616 Purity: 99.78%
COA
SDS
Handling Instructions Technical Support

Benzylacetone (4-Penylbutan-2-one) is an aromatic compound. Benzylacetone is a mushroom tyrosinase inhibitor with an IC50 of 2.8 mM, a Ki of 1.25 mM for monophenolase and an IC50 of 0.6 mM, a Ki of 0.39 mM for diphenolase. Benzylacetone inhibits free mushroom tyrosinase and enzyme-substrate complex. Benzylacetone acts as an appetite enhancer via olfactory stimulation, reduces spontaneous locomotor activity, induces weight gain. Benzylacetone exhibits repellent, fumigant, and contact toxicity against Tribolium castaneum adults.

For research use only. We do not sell to patients.

Benzylacetone

Benzylacetone Chemical Structure

CAS No. : 2550-26-7

Size Price Stock Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Benzylacetone:

Benzylacetone Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Benzylacetone (4-Penylbutan-2-one) is an aromatic compound. Benzylacetone is a mushroom tyrosinase inhibitor with an IC50 of 2.8 mM, a Ki of 1.25 mM for monophenolase and an IC50 of 0.6 mM, a Ki of 0.39 mM for diphenolase. Benzylacetone inhibits free mushroom tyrosinase and enzyme-substrate complex. Benzylacetone acts as an appetite enhancer via olfactory stimulation, reduces spontaneous locomotor activity, induces weight gain. Benzylacetone exhibits repellent, fumigant, and contact toxicity against Tribolium castaneum adults[1][2][3].

IC50 & Target

IC50: 2.8 mM (monophenolase, tyrosinase mushroom), 0.6 mM (diphenolase, tyrosinase mushroom)[2]
Cellular Effect
Cell Line Type Value Description References
Raji IC50
222 molar ratio
Compound: 58
Cytotoxicity against human Raji cells expressing EBV-EA assessed as inhibition of TPA-induced EBV-EA activation after 48 hrs by trypan blue staining based immunofluorescence method relative to TPA
Cytotoxicity against human Raji cells expressing EBV-EA assessed as inhibition of TPA-induced EBV-EA activation after 48 hrs by trypan blue staining based immunofluorescence method relative to TPA
[PMID: 26796952]
In Vitro

Benzylacetone (0-3.0 mM) reversibly inhibits mushroom tyrosinase diphenolase activity as a mixed-type inhibitor with an IC50 of 0.6 mM and a Ki of 0.39 mM[1].
Benzylacetone (0-5.0 mM) reversibly inhibits mushroom tyrosinase monophenolase activity as a mixed-type inhibitor with an IC50 of 1.5 mM, and a Ki of 1.25 mM, without significantly altering the reaction lag time, and it primarily inhibits the enzyme-substrate complex[1].
Benzylacetone (0.13-78.63 nL/cm2; 2-4 h) exhibits dose-dependent and time-dependent repellent activity against 1-2 week old adult Tribolium castaneum, and sustained activity even at low concentrations after 4 h exposure[3].
Benzylacetone (1.875-30% (v/v); 6 h) exhibits concentration-dependent fumigation toxicity against 1-2 week old adult Tribolium castaneum, with an LC50 of 122.15 mg/L air[3].
Benzylacetone (1.875-30% (v/v); 6 h) exhibits contact toxicity against 1-2 week old adult Tribolium castaneum, with an LD50 of 25.54 μg/adult[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Benzylacetone Related Antibodies
In Vivo

Benzylacetone (7.4×10-8-7.4×10-2 mg/L; inhalation; 5-60 min) produces significant, time- and concentration-dependent appetite enhancement in fasted male ddY mice[2].
Benzylacetone (inhalation; once daily; 14 days, 60 min per day) produces significant weight gain in healthy male ddY mice, with a total weight gain approximately 2.5 g greater than controls and significantly elevated body weights measured on most days of the study[2].
Benzylacetone (7.4×10-7-7.4×10-3 mg/L; inhalation; 60 min) produces significant, reduction in locomotor activity in healthy male ddY mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: ddY mice (male, 4-week-old at purchase, 19 g at experiment start, fasted)[2]
Dosage: 7.4×10-8 mg/L; 7.4×10-7 mg/L; 7.4×10-6 mg/L; 7.4×10-5 mg/L; 7.4×10-4 mg/L; 7.4×10-3 mg/L; 7.4×10-2 mg/L
Administration: Inhalation; 5, 15, 30, 60 min
Result: Produced significant appetite-enhancing effects at doses of 7.4×10-6 to 7.4×10-4 mg/L, with the strongest effect at 7.4×10-6 mg/L.
Increased food intake 1.158-fold (15 min inhalation), 1.203-fold (30 min inhalation), and 1.241-fold (60 min inhalation) compared to controls at 7.4×10-6 mg/L.
Increased food intake 1.2-fold compared to controls at 7.4×10-3 mg/L for 5 min.
Decreased food intake lower than controls at the highest dose tested (7.4×10-2 mg/L).
Molecular Weight

148.21

Formula

C10H12O
CAS No.
Appearance

Liquid (Density: 0.989  g/cm3  )

Color

Colorless to off-white

SMILES

CC(CCC1=CC=CC=C1)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (674.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.7472 mL 33.7359 mL 67.4718 mL
5 mM 1.3494 mL 6.7472 mL 13.4944 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (16.87 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (16.87 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.78%

SDS (393 KB)

COA (245 KB) HNMR (231 KB) LCMS (89 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.7472 mL 33.7359 mL 67.4718 mL 168.6796 mL
5 mM 1.3494 mL 6.7472 mL 13.4944 mL 33.7359 mL
10 mM 0.6747 mL 3.3736 mL 6.7472 mL 16.8680 mL
15 mM 0.4498 mL 2.2491 mL 4.4981 mL 11.2453 mL
20 mM 0.3374 mL 1.6868 mL 3.3736 mL 8.4340 mL
25 mM 0.2699 mL 1.3494 mL 2.6989 mL 6.7472 mL
30 mM 0.2249 mL 1.1245 mL 2.2491 mL 5.6227 mL
40 mM 0.1687 mL 0.8434 mL 1.6868 mL 4.2170 mL
50 mM 0.1349 mL 0.6747 mL 1.3494 mL 3.3736 mL
60 mM 0.1125 mL 0.5623 mL 1.1245 mL 2.8113 mL
80 mM 0.0843 mL 0.4217 mL 0.8434 mL 2.1085 mL
100 mM 0.0675 mL 0.3374 mL 0.6747 mL 1.6868 mL
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Benzylacetone Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Benzylacetone2550-26-74-Penylbutan-2-oneTyrosinaseEnvironmental PollutantsEnvironmental ContaminantsmelasmaddY micefrecklesTribolium castaneummonophenolasediphenolaseage spotsmushroom tyrosinaseInhibitorinhibitorinhibit

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