Art. -Nr.: HY-108469

BI-6015

5 Cited Publications

Kundenbewertung

Based on 5 publication(s) in Google Scholar

BI-6015 is a hepatocyte nuclear factor 4α (HNF4α) antagonist that can inhibit the expression of known HNF4α target genes. BI6015 represses insulin promoter activity through HNF4α antagonism. BI-6015 can be used for the research of cancer and diabetes.

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Reinheit: 99.90%
CAS. Nr.: 93987-29-2
Formel: C₁₅H₁₃N₃O₄S
Molecular Weight:331.35

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

BI-6015 Chemical Structure

CAS. Nr.: 93987-29-2

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (5)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

hepatocyte nuclear factor 4α (HNF4α)^[1]

In Vitro

BI-6015 (1.25-20 μM; 24-72 h) is cytotoxic to human hepatocellular carcinoma (HCC)^[1].
BI-6015 (2.5-10 μM; 5-48 h) inhibits HNF4α gene expression in HepG2 cells^[1].
BI-6015 (5 μM; 3 d) induces hepatic steatosis in primary murine hepatocytes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Hep3B-Luc cells and primary hepatocytes
Concentration:

1.25, 2.5, 5, 10, 20 μM
Incubation Time:

24, 48, 72 hours
Result:

Was markedly toxic to Hep3B cells but spared primary hepatocytes.

In Vivo

BI-6015 (10-30 mg/kg; i.p. once daily for 5 days) induces loss of HNF4α expression and hepatic steatosis in mice^[1].
BI-6015 (10-30 mg/kg; i.p. daily or every other day for 20-57 days) induces apoptosis in a human hepatocellular carcinoma mouse model^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 93987-29-2
Appearance Solid
Molecular Weight 331.35
Formel C₁₅H₁₃N₃O₄S
Color White to off-white
SMILES

O=S(N1C2=CC=CC=C2N=C1C)(C3=CC([N+]([O-])=O)=CC=C3C)=O
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (5)

All RT-PCRWBBio/Physico-chemical AssayIFCell Proliferation/Viability Assay

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Journal Impact Factor

Mol Syst Biol

Systemic genome-epigenome analysis captures a lineage-specific super-enhancer for MYB in gastrointestinal adenocarcinoma. [Abstract]

2025 Apr 15. PMID: 40234694

BI-6015 purchased from MedChemExpress. Usage Cited in: Mol Syst Biol. 2025 Apr 15. [Abstract]

The expression of HNF4A and MYB upon the treatment of HNF4A antagonist BI-6015 (0-5 μM) in HT-55 cells.

BI-6015 purchased from MedChemExpress. Usage Cited in: Mol Syst Biol. 2025 Apr 15. [Abstract]

Luciferase reporter assay measuring the activity of theenhancer e4 following the treatment of HNF4A antagonist BI-6015 (0 and 5 μM) in HT-55 cells.

BI-6015 purchased from MedChemExpress. Usage Cited in: Mol Syst Biol. 2025 Apr 15. [Abstract]

Cell viability of HT-55 cells after 48 h treatment of BI-6015 (0-5 μM), measured by Cell Counting Kit-8 (CCK-8).
Int Immunopharmacol

Wogonoside ameliorates oxidative damage in tubular epithelial cells of diabetic nephropathy by modulating the HNF4A-NRF2 axis. [Abstract]

2025 Apr 16:152:114481. PMID: 40086061
Elife

Transcriptional regulation of SARS-CoV-2 receptor ACE2 by SP1. [Abstract]

2024 Feb 20:13:e85985. PMID: 38375778

BI-6015 purchased from MedChemExpress. Usage Cited in: Elife. 2024 Feb 20:13:e85985. [Abstract]

Supplementation with colchicine (20 nM) significantly suppressed ACE2 levels in HPAEpiCs, which was reversed by treatment with BI6015 (20 μM) or siHNF4α.

BI-6015 purchased from MedChemExpress. Usage Cited in: Elife. 2024 Feb 20:13:e85985. [Abstract]

HPAEpiCs were treated with MithA (100 nM), BI6015 (20 μM), colchicine (20 nM), colchicine + MithA, or colchicine +BI6015, respectively. Representative images of immunofluorescence staining for ACE2 in HPAEpiCs.
bioRxiv

2023 Feb 14.
Research Square Print

December 19th, 2022.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 50 mg/mL (150.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.0180 mL

15.0898 mL

30.1796 mL

5 mM

0.6036 mL

3.0180 mL

6.0359 mL

10 mM

0.3018 mL

1.5090 mL

3.0180 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (7.54 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Kiselyuk A, et, al. HNF4α antagonists discovered by a high-throughput screen for modulators of the human insulin promoter. Chem Biol. 2012 Jul 27;19(7):806-18. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.0180 mL	15.0898 mL	30.1796 mL	75.4489 mL
	5 mM	0.6036 mL	3.0180 mL	6.0359 mL	15.0898 mL
	10 mM	0.3018 mL	1.5090 mL	3.0180 mL	7.5449 mL
	15 mM	0.2012 mL	1.0060 mL	2.0120 mL	5.0299 mL
	20 mM	0.1509 mL	0.7545 mL	1.5090 mL	3.7724 mL
	25 mM	0.1207 mL	0.6036 mL	1.2072 mL	3.0180 mL
	30 mM	0.1006 mL	0.5030 mL	1.0060 mL	2.5150 mL
	40 mM	0.0754 mL	0.3772 mL	0.7545 mL	1.8862 mL
	50 mM	0.0604 mL	0.3018 mL	0.6036 mL	1.5090 mL
	60 mM	0.0503 mL	0.2515 mL	0.5030 mL	1.2575 mL
	80 mM	0.0377 mL	0.1886 mL	0.3772 mL	0.9431 mL
	100 mM	0.0302 mL	0.1509 mL	0.3018 mL	0.7545 mL

Keywords

BI-6015

5 Cited Publications

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BI-6015

GPCR/G Protein Compound Library

Anti-Cancer Compound Library

Anti-diabetic Compound Library

Nuclear Receptor Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) BI-6015

Biologische Aktivität

Chemical Information

Publications (5)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

BI-6015

GPCR/G Protein Compound Library

Anti-Cancer Compound Library

Anti-diabetic Compound Library

Nuclear Receptor Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) BI-6015

Biologische Aktivität

Chemical Information

Publications (5)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

BI-6015 Related Classifications

HY-108469 Related Classifications