Cat. No.: HY-17468A

Bumetanide sodium

(Synonyms: Ro 10-6338 sodium; PF 1593 sodium)

19 Cited Publications

Customer Review

Based on 19 publication(s) in Google Scholar

Bumetanide sodium, a highly potent loop diuretic, is a Na⁺-K⁺-Cl⁺ cotransporter (NKCC) blocker. Bumetanide sodium is a selective NKCC1 inhibitor, and also inhibits NKCC2, with IC₅₀s of 0.68 and 4.0 μM for hNKCC1A and hNKCC2A, respectively.

For research use only. We do not sell to patients.

CAS No.: 28434-74-4
Formula: C₁₇H₁₉N₂NaO₅S
Molecular Weight:386.40

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Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (276 KB)

Bumetanide sodium Chemical Structure

CAS No.: 28434-74-4

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Biological Activity
- Description
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (19)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

In Vitro

Bumetanide sodium has inhibitory effects for the two major human splice variants of NKCCs, hNKCC1A and hNKCC2A ^[1].
Bumetanide sodium (0.03-100 μM; 5 minutes) inhibits the ⁸⁶Rb⁺ uptake in NKCC1A-expressing oocytes in a dose-dependent manner^[1].
Bumetanide sodium inhibits NKCC2 isoform B in HEK-293 cells with an IC₅₀ value of 0.54 μM^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Bumetanide sodium (7.6-30.4 mg/kg; i.v.) attenuates the decrease in apparent diffusion coefficients (ADC) ratios for both cortex and striatum (by 40-67%), indicating reduced edema formation^[3].
Bumetanide sodium also reduces infarct size^[3].
Bumetanide sodium shows different half-lives of 21.4 min, 53.8 min and 137 min following 2 mg/kg, 8 mg/kg and 20 mg/kg intravenous injection, respectively, in rats^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 28434-74-4
Molecular Weight 386.40
Formula C₁₇H₁₉N₂NaO₅S
SMILES

O=C(O[Na])C1=CC(NCCCC)=C(OC2=CC=CC=C2)C(S(=O)(N)=O)=C1
Synonyms

Ro 10-6338 sodium; PF 1593 sodium
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (19)

All IFBio/Physico-chemical AssayWBIn Vivo Efficacy Study

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J Neuroinflammation

NLRP3 inflammasome-mediated choroid plexus hypersecretion contributes to hydrocephalus after intraventricular hemorrhage via phosphorylated NKCC1 channels. [Abstract]

2022 Jun 21;19(1):163. PMID: 35729645

Bumetanide sodium purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2022 Jun 21;19(1):163. [Abstract]

Representative images of immunofluorescence staining for p-NKCC1 and p-NKCC1-positive cell statistics of the choroid plexus at 3 days following Bumetanide treatment (6 rats/group, Welch's two-tailed unpaired t-test). Bar = 50 μm.

Bumetanide sodium purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2022 Jun 21;19(1):163. [Abstract]

Western blot analysis of p-NKCC1 and NLRP3 in the choroid plexus at 3 days after Bumetanide treatment.

Bumetanide sodium purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2022 Jun 21;19(1):163. [Abstract]

CSF secretion rates after Bumetanide treatment (6-8 rats/group, Welch's two-tailed unpaired t-test).
EMBO J

Structural basis for human NKCC1 inhibition by loop diuretic drugs. [Abstract]

2025 Mar;44(5):1540-1562. PMID: 39875725

Bumetanide sodium purchased from MedChemExpress. Usage Cited in: EMBO J. 2025 Mar;44(5):1540-1562. [Abstract]

NKCC1-mediated Cl^- influx was inhibited by furosemide, Bumetanide, and torsemide applied externally to the cells at 100 μM concentration.
Biochem Pharmacol

Sodium-potassium-chloride cotransporter 1 as a novel regulator of DAMPs-mediated alveolar epithelial-macrophage crosstalk in LPS-induced lung inflammation. [Abstract]

2026 Mar:245:117671. PMID: 41506546
Glia

Endothelin-1 increases Na+-K+-2Cl- cotransporter-1 expression in cultured astrocytes and in traumatic brain injury model: An involvement of HIF1α activation. [Abstract]

2024 Dec;72(12):2231-2246. PMID: 39166289
Eur J Pharmacol

Loop diuretics mitigate juvenile immobilization treatment-induced hippocampal dysfunction. [Abstract]

2025 Jun 5:996:177447. PMID: 40023355
J Physiol

The apical mucus layer alters the pharmacological properties of the airway epitheliumy. [Abstract]

2025 May;603(9):2619-2632. PMID: 40047394

Bumetanide sodium purchased from MedChemExpress. Usage Cited in: J Physiol. 2025 May;603(9):2619-2632. [Abstract]

Representative short-circuit current traces (left) and summary graphs (right) from epithelia kept in control conditions, treated with IL-4 or treated with IL-17A/TNF-α. During recordings, CFTR_inh-172 (apical, 10 µM) and Bumetanide (basolateral, 100 µM) were added sequentially after stimulation of CFTR with CPT-cAMP. Dotted and dashed lines indicate the baseline for cAMP-activated current and zero current level, as in previous legends. The graphs report the fraction of the cAMP-activated current blocked by CFTR_inh-172 (I-172), bumetanide alone (bum) or both compounds together (tot).
Exp Neurol

Mechanisms of polygalasaponin F against brain ischemia-reperfusion injury by targeting NKCC1. [Abstract]

2024 Nov 26:115076. PMID: 39608559
iScience

Dim light at night induces cardiac injury in zebrafish embryos via disrupted chloride homeostasis. [Abstract]

2026 Jun 3;29(6):115796. PMID: 42291255
Cell Calcium

Activation of TRPM8 promotes K+ secretion in rat epididymal epithelium. [Abstract]

2026 Mar:134:103117. PMID: 41539212
Biomedicines

Characterization of a Clinically and Biologically Defined Subgroup of Patients with Autism Spectrum Disorder and Identification of a Tailored Combination Treatment. [Abstract]

2024 Apr 30;12(5):991. PMID: 38790952
J Neurotrauma

Attenuation of Blood-Brain Barrier Disruption in Traumatic Brain Injury via Inhibition of NKCC1 Cotransporter: Insights into the NF-κB/NLRP3 Signaling Pathway. [Abstract]

2025 May;42(9-10):814-831. PMID: 39879999
Viruses

Identification of Oncolytic Avian Reovirus Receptors in B16-F10 Cells and the Signaling-Mediated Pathways Involved in Viral Entry. [Abstract]

2026 Mar 12;18(3):350. PMID: 41902258
Andrology

Cellular mechanism underlying leptin-induced anion secretion of rat epididymal epithelial cells. [Abstract]

2024 May 22. PMID: 38778669
J Pharm Biomed Anal

Screening method of mildronate and over 300 doping agents by reversed-phase liquid chromatography-high resolution mass spectrometry. [Abstract]

2021 Feb 20:195:113870. PMID: 33453569
Biochem Biophys Res Commun

Glucose transports in the ileum: mechanism, regulation and physiological role of ileal glucose absorption. [Abstract]

2025 Sep 19:785:152676. PMID: 41005286
Res Sq

2024 Jun 11.
bioRxiv

2024 June 12.
bioRxiv

2024 May 13.
Research Square Preprint

2020 Nov.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Lykke K, et al. The search for NKCC1-selective drugs for the treatment of epilepsy: Structure-function relationship of bumetanide and various bumetanide derivatives in inhibiting the human cation-chloride cotransporter NKCC1A. Epilepsy Behav. 2016 Jun;59: [Content Brief]

[2]. Ciaran Richardson, et al. Regulation of the NKCC2 ion cotransporter by SPAK-OSR1-dependent and -independent pathways. J Cell Sci. 2011 Mar 1;124(Pt 5):789-800. [Content Brief]

[3]. Martha E O'Donnell, et al. Bumetanide inhibition of the blood-brain barrier Na-K-Cl cotransporter reduces edema formation in the rat middle cerebral artery occlusion model of stroke. J Cereb Blood Flow Metab. 2004 Sep;24(9):1046-56. [Content Brief]

[4]. S H Lee, et al. Pharmacokinetics and pharmacodynamics of bumetanide after intravenous and oral administration to rats: absorption from various GI segments. J Pharmacokinet Biopharm. 1994 Feb;22(1):1-17.6 [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bumetanide sodium

19 Cited Publications

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Bumetanide sodium

Publications Citing Use of MedChemExpress (MCE) Bumetanide sodium

Biological Activity

Chemical Information

Publications (19)

Purity & Documentation

References

Calculators

Help & FAQs

Bumetanide sodium Related Classifications

HY-17468A Related Classifications