1. Membrane Transporter/Ion Channel
  2. NKCC
  3. Bumetanide

Bumetanide (Synonyms: Ro 10-6338; PF 1593)

Cat. No.: HY-17468 Purity: 99.91%
Handling Instructions

Bumetanide(Ro 10-6338; PF 1593) is an inhibitor of Na(+)-K(+)-2Cl(-) co-transporter (NKCC) with an IC50 of 0.6 uM.

For research use only. We do not sell to patients.

Bumetanide Chemical Structure

Bumetanide Chemical Structure

CAS No. : 28395-03-1

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10 mM * 1 mL in DMSO USD 117 In-stock
Estimated Time of Arrival: December 31
1 g USD 106 In-stock
Estimated Time of Arrival: December 31
5 g USD 432 In-stock
Estimated Time of Arrival: December 31
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Description

Bumetanide(Ro 10-6338; PF 1593) is an inhibitor of Na(+)-K(+)-2Cl(-) co-transporter (NKCC) with an IC50 of 0.6 uM. IC50 Value: 0.6 uM [1] Target: NKCC (Na-K-Cl cotransporter) in vitro: Cultured chick cardiac cells possess a Na+K+Cl-co-transport system that is inhibited by the "loop diuretics" bumetanide (IC50 = 0.6 microM). The K0.5 values for Cl- and Na+ activation of thebumetanide-sensitive 86Rb+ uptake are 59 mM and 40mM respectively. Bumetanide also inhibits a 22Na+ uptake component that is suppressed when external Cl- or K+ are substituted by impermeant ions. The ratio of bumetanide-sensitive 86Rb+ to 22Na+ uptake is close to 1. The cardiac Na+/K+/Cl- cotransport is a major uptake pathway for Na+ and K+ [1]. Bumetanide inhibits ouabain-resistant 86Rb(K+) influx with IC50of 0.1, 5.0, and 0.05 microM for J774.2, CT2 and J7H1 macrophages, respectively [2]. in vivo:Intraperitoneal injection of 50 or 100 mg bumetanide/kg body weight resulted in an acute and transient hyperglycaemia. Pretreatment with 240 mg probenecid/kg body weight reduced the diuretic effect but potentiated the hyperglycaemic effect of bumetanide (50 mg/kg body weight). The glucose tolerance was impaired, and there was an elevated serum glucose and glucose/insulin ratio 2 h after a single injection of bumetanide (100 mg/kg body weight) [3].

Clinical Trial
Storage

4°C, protect from light

Solvent & Solubility
In Vitro: 

DMS : ≥ 100 mg/mL (274.41 mM)

H2O : < 0.1 mg/mL (insoluble)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7441 mL 13.7204 mL 27.4409 mL
5 mM 0.5488 mL 2.7441 mL 5.4882 mL
10 mM 0.2744 mL 1.3720 mL 2.7441 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (6.86 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (6.86 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Molecular Weight

364.42

Formula

C₁₇H₂₀N₂O₅S

CAS No.

28395-03-1

SMILES

O=C(O)C1=CC(NCCCC)=C(OC2=CC=CC=C2)C(S(=O)(N)=O)=C1

Shipping

Room temperature in continental US; may vary elsewhere

Purity: 99.91%

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Product Name:
Bumetanide
Cat. No.:
HY-17468
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Bumetanide

Cat. No.: HY-17468