2. Membrane Transporter/Ion Channel Autophagy
  3. CFTR Autophagy
  4. CFTR(inh)-172

CFTR(inh)-172 is a potent and selective blocker of the CFTR chloride channel; reversibly inhibits CFTR short-circuit current in less than 2 minutes with a Ki of 300 nM.

For research use only. We do not sell to patients.

CAS No. : 307510-92-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
1 mg In-stock
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 23 publication(s) in Google Scholar

CFTR(inh)-172 Related Antibodies

Top Publications Citing Use of Products

    CFTR(inh)-172 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2026 Jan 8;17(1):1253.  [Abstract]

    Procedure to determine the effect of CaCCinh-A01 (A01; 3 mg/kg), an ANO1 inhibitor and CFTRinh-172 (172; 1 mg/kg, i.p., 30 min prior to the administration of DAB, every 12 h for 24 h), a CFTR inhibitor on DAB-induced laxative effect.

    CFTR(inh)-172 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug 21:e01661.  [Abstract]

    Bright‐field images of cystic kidney organoids treated with chloride channel inhibitors CFTR inhibitor‐172 (CFTRinh‐172, 50-100 μM) for 24 h. The cystic kidney organoids were generated by treating organoids with forskolin 30 µm for 48 h.

    CFTR(inh)-172 purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2024 Jan 16;134(2):e171249.  [Abstract]

    Isc traces in T84 cells showing the forskolin concentration response and CFTRinh-172 (10 μM) inhibition following 20 minutes of pretreatment with the indicated concentrations of CaCl2 or 30 μM cinacalcet (left). Summary of changes in Isc (Δ Isc) from the experiments (right).

    CFTR(inh)-172 purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2024 Jan 16;134(2):e171249.  [Abstract]

    Δ Isc induced by CFTRinh-172 in the presence of different Ca2+ concentrations and cinacalcet. n = 3–6 per group. Data indicate the mean ± SEM.

    CFTR(inh)-172 purchased from MedChemExpress. Usage Cited in: Cell Stem Cell. 2019 Sep 5;25(3):373-387.e9.  [Abstract]

    Representative bright-field images of ARPKD kidney organoids treated with various concentrations of CFTRinh172 (50 µM to 100 µM) in the absence or presence of 10 μM FSK for 3 days (Day 28 to 31). Scale bars, 500 μm.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    CFTR(inh)-172 is a potent and selective blocker of the CFTR chloride channel; reversibly inhibits CFTR short-circuit current in less than 2 minutes with a Ki of 300 nM.

    IC50 & Target

    Ki: 300 nM (CFTR)[1]
    In Vitro

    Inhibition by CFTR(inh)-172 is complete in approximately 10 minutes (t1/2=4 minutes) and is reversed after ishout with t1/2 approximately 5 minutes. CFTRinh-172 is nontoxic to FRT cells after 24 hours at concentrations up to 100 μM[1]. CFTR(inh)-172 does not alter CFTR unitary conductance (8 pS), but reduces open probability by > 90% with Ki=0.6 μM. This effect is due to increased mean channel closed time without changing mean channel open time. The Ki values for inhibition of Cl- current in wild-type, G551D, and G1349D CFTR are about 0.5 μM; however, Ki is significantly reduced to 0.2 μM for vF508 CFTR[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    CFTR(inh)-172 Related Antibodies
    In Vivo

    A single intraperitoneal injection of CFTR(inh)-172 (250 μg/kg) in mice reduces by more than 90% cholera toxin–induced fluid secretion in the small intestine over 6 hours. CFTR(inh)-172 is nontoxic at high concentrations in mouse models. CFTRinh-172 significantly reduces fluid secretion to that in saline control loops, whereas an inactive CFTRinh-172 analog does not inhibit fluid secretion[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    409.40

    Formula

    C18H10F3NO3S2
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    S=C(S/1)N(C2=CC=CC(C(F)(F)F)=C2)C(C1=C\C3=CC=C(C=C3)C(O)=O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (122.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4426 mL 12.2130 mL 24.4260 mL
    5 mM 0.4885 mL 2.4426 mL 4.8852 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2.5 mg/mL (6.11 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (6.11 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.80%

    SDS (761 KB)

    COA (249 KB) RP-HPLC (244 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [1]

    CFTR(inh)-172 is diluted in DMSO as a 10 mM stock solution and diluted with appropriate medium. Fischer rat thyroid (FRT) cells coexpressing human wild-type CFTR and the halide indicator YFP-H148Q are generated. Cell toxicity is assayed by the dihydrorhodamine method at 24 hours after cell incubation with 0–1,000 μM inhibitor CFTR(inh)-172[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [1]

    Mice: Animal toxicity is assessed by measurement of serum chemistries and hematology in mice at 5 days after daily intraperitoneal injections with 0-1,000 μg/kg CFTR(inh)-172[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.4426 mL 12.2130 mL 24.4260 mL 61.0650 mL
    5 mM 0.4885 mL 2.4426 mL 4.8852 mL 12.2130 mL
    10 mM 0.2443 mL 1.2213 mL 2.4426 mL 6.1065 mL
    15 mM 0.1628 mL 0.8142 mL 1.6284 mL 4.0710 mL
    20 mM 0.1221 mL 0.6106 mL 1.2213 mL 3.0532 mL
    25 mM 0.0977 mL 0.4885 mL 0.9770 mL 2.4426 mL
    30 mM 0.0814 mL 0.4071 mL 0.8142 mL 2.0355 mL
    40 mM 0.0611 mL 0.3053 mL 0.6106 mL 1.5266 mL
    50 mM 0.0489 mL 0.2443 mL 0.4885 mL 1.2213 mL
    60 mM 0.0407 mL 0.2035 mL 0.4071 mL 1.0177 mL
    80 mM 0.0305 mL 0.1527 mL 0.3053 mL 0.7633 mL
    100 mM 0.0244 mL 0.1221 mL 0.2443 mL 0.6106 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    CFTR(inh)-172 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    CFTR(inh)-172307510-92-5CFTRAutophagyCystic fibrosis transmembrane conductance regulatorInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    CFTR(inh)-172
    Cat. No.:
    HY-16671
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (761 KB) Français - FR (761 KB) Deutsch - DE (761 KB) Norwegian - NO (761 KB) Español - ES (761 KB) Swedish - SV (761 KB) Italian - IT (761 KB) Korean - KR (761 KB) Portuguese - PT (761 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.