1. Membrane Transporter/Ion Channel
  2. NKCC
  3. Azosemide

Azosemide 

Cat. No.: HY-107321 Purity: >98.0%
Handling Instructions

Azosemide, a sulfonamide loop diuretic, is a potent NKCC1 inhibitor with IC50s of 0.246 µM and 0.197 µM for hNKCC1A and NKCC1B, respectively.

For research use only. We do not sell to patients.

Azosemide Chemical Structure

Azosemide Chemical Structure

CAS No. : 27589-33-9

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
10 mg USD 50 In-stock
Estimated Time of Arrival: December 31
25 mg USD 85 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

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Description

Azosemide, a sulfonamide loop diuretic, is a potent NKCC1 inhibitor with IC50s of 0.246 µM and 0.197 µM for hNKCC1A and NKCC1B, respectively[1].

IC50 & Target

IC50: 0.246 µM (hNKCC1A) and 0.197 µM (NKCC1B)[1]

In Vitro

Azosemide inhibits the sodium-potassium-chloride-cotransporter human variants hNKCC1A and hNKCC1B[1].

In Vivo

Azosemide shows a smaller AUC (81.9% decrease), shorter terminal half-life (50.9% decrease) and MRT (64.1% decrease), faster CL (454% increase), CLR (853% increase) and CLNR (307% increase) for NARs[2].

Animal Model: Male Sprague-Dawley rats (control rats, weighing 310345 g) and NARs (weighing 220315 g) of 9 weeks of age[2]
Dosage: 10 mg/kg (Pharmacokinetic Analysis)
Administration: Infused over 1 min via the jugular vein (i.v.)
Result: Showed a smaller AUC (81.9% decrease), shorter terminal half-life (50.9% decrease) and MRT (64.1% decrease), faster CL (454% increase), CLR (853% increase) and CLNR (307% increase) for NARs.
Molecular Weight

370.84

Formula

C₁₂H₁₁ClN₆O₂S₂

CAS No.

27589-33-9

SMILES

ClC1=CC(NCC2=CC=CS2)=C(C3=NN=NN3)C=C1S(N)(=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (674.15 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6966 mL 13.4829 mL 26.9658 mL
5 mM 0.5393 mL 2.6966 mL 5.3932 mL
10 mM 0.2697 mL 1.3483 mL 2.6966 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.17 mg/mL (5.85 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.17 mg/mL (5.85 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

AzosemideNKCCNa-K-Cl cotransporterNa(+)-K(+)-Cl(-) cotransporterNa+-K+-Cl− cotransportersulfonamideloopdiureticNKCC1hNKCC1ANKCC1BInhibitorinhibitorinhibit

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Product name:
Azosemide
Cat. No.:
HY-107321
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