BODIPY−DOX
BODIPY-DOX is a conjugate composed of BODIPY and Doxorubicin (HY-15142A), as well as a pH-activated fluorescent probe for M1 macrophages and an apoptosis inducer. BODIPY-DOX undergoes pH-induced hydrazone bond cleavage in acidic M1 macrophage phagosomes, thereby releasing cytotoxic Doxorubicin (Dox) and inhibiting the function of pro-inflammatory M1 macrophages. BODIPY-DOX highly selectively inhibits the production of relevant pro-inflammatory cytokines by mouse and human monocyte-derived M1 macrophages, while exerting minimal effects on M2 or unactivated macrophages. Therefore, BODIPY-DOX enables simultaneous fluorescent tracing, differentiation and elimination of specific macrophage subsets, and exhibits the potential to regulate tissue regeneration in zebrafish models.
For research use only. We do not sell to patients.
- CAS No.: 2173116-67-9
- Formula: C54H62BF2N7O12
- Molecular Weight:1049.92
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
BODIPY-DOX (5-10 μM; overnight) induces dose-dependent cytotoxicity in LPS-polarized M1 RAW264.7 murine macrophages, but does not affect cell viability in nonactivated or IL-4-polarized M2 RAW264.7 murine macrophages[1].
BODIPY-DOX (10 μM; 20 h) activates to release fluorescent signal and induce apoptosis specifically in LPS-polarized M1 RAW264.7 murine macrophages, with minimal effects on nonactivated RAW264.7 murine macrophages[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:LPS-polarized M1 RAW264.7 murine macrophages, IL-4-polarized M2 RAW264.7 murine macrophages, nonactivated RAW264.7 murine macrophages
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Concentration:5 μM, 10 μM
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Incubation Time:overnight
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Result:Reduced normalized cell viability of M1 macrophages to ~0.8.
Reduced normalized cell viability of M1 macrophages to ~0.65.
Did not reduce normalized cell viability in nonactivated or M2 macrophages at either concentration.
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Cell Line:LPS-polarized M1 RAW264.7 murine macrophages, nonactivated RAW264.7 murine macrophages
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Concentration:10 μM
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Incubation Time:20 h
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Result:Showed strong BODIPY fluorescence staining in LPS-polarized M1 macrophages.
Increased apoptotic index (Annexin V-positive cells) of LPS-polarized M1 macrophages to 3-fold higher than that of nonactivated macrophages.
Exhibited coincident strong BODIPY fluorescence in apoptotic M1 macrophages.
Showed low BODIPY fluorescence staining and a low apoptotic index in nonactivated macrophages.
Chemical Information
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CAS No. 2173116-67-9
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Molecular Weight 1049.92
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Formula C54H62BF2N7O12
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SMILES
[H][C@@]1(C[C@](/C(CO)=N\NC(CCC2=[N+]3C(C(C)=C2)=C(C4=C(C=C(N4[B-]3(F)F)C)C)C5=CC=C(C=C5)NC(CN(CC)CC)=O)=O)(CC6=C(C7=C(C(O)=C61)C(C8=C(C=CC=C8C7=O)OC)=O)O)O)O[C@H]9C[C@@H]([C@@H]([C@@H](O9)C)O)N
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
BODIPY−DOX Related Classifications
HY-D3190 Related Classifications
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