DFHBI-2T
Based on 1 Customer Validation
DFHBI-2T is a membrane-permeable, RNA aptamer-activated fluorescent probe (Ex/Em=500 nm/523 nm), with a Kd value of 1300 nM for the RNA aptamer Spinach2. DFHBI-2T binds to Spinach2, converting the fluorophore to a fluorescent state, stabilizing its planar structure to facilitate the radiative fluorescence decay pathway, and forming a complex with red-shifted excitation and emission peaks. DFHBI-2T can be used for RNA imaging in live cells. It is also applicable to research related to fragile X-associated tremor/ataxia syndrome.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 1539318-40-5
- Formula: C12H7F5N2O2
- Molecular Weight:306.19
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
DFHBI-2T (1 μM) binds Spinach2 RNA aptamer with a Kd of 1300 nM, forming a complex with excitation maximum at 500 nm, emission maximum at 523 nm[1].
DFHBI-2T (20 μM; 10 min) forms a fluorescent complex that is readily detectable using a YFP filter cube but not a GFP filter cube in COS7 cells expressing (CGG)60-Spinach2[1].
DFHBI-2T enables SAM-dependent fluorescence detection at 523 nm using a Spinach2-based SAM sensor in vitro[1].
DFHBI-2T binds to the Spinach2 RNA aptamer with a Kd of 1300 nM, exhibiting a shift in spectral properties and a 60-fold increase in brightness compared to its free state[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1539318-40-5
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Appearance Solid
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Molecular Weight 306.19
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Formula C12H7F5N2O2
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Color Light yellow to yellow
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SMILES
O=C1N(C)C(C(F)(F)F)=N/C1=C\C2=CC(F)=C(O)C(F)=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (326.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2659 mL | 16.3297 mL | 32.6595 mL | 81.6486 mL |
| 5 mM | 0.6532 mL | 3.2659 mL | 6.5319 mL | 16.3297 mL | |
| 10 mM | 0.3266 mL | 1.6330 mL | 3.2659 mL | 8.1649 mL | |
| 15 mM | 0.2177 mL | 1.0886 mL | 2.1773 mL | 5.4432 mL | |
| 20 mM | 0.1633 mL | 0.8165 mL | 1.6330 mL | 4.0824 mL | |
| 25 mM | 0.1306 mL | 0.6532 mL | 1.3064 mL | 3.2659 mL | |
| 30 mM | 0.1089 mL | 0.5443 mL | 1.0886 mL | 2.7216 mL | |
| 40 mM | 0.0816 mL | 0.4082 mL | 0.8165 mL | 2.0412 mL | |
| 50 mM | 0.0653 mL | 0.3266 mL | 0.6532 mL | 1.6330 mL | |
| 60 mM | 0.0544 mL | 0.2722 mL | 0.5443 mL | 1.3608 mL | |
| 80 mM | 0.0408 mL | 0.2041 mL | 0.4082 mL | 1.0206 mL | |
| 100 mM | 0.0327 mL | 0.1633 mL | 0.3266 mL | 0.8165 mL |
DFHBI-2T Related Classifications
HY-110251A Related Classifications
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