Posovolone
Based on 1 Customer Validation
Posovolone (Co 134444) is an orally active, neuroactive steroid. Posovolone has anticonvulsant and anxiolytic-like activity as well as ataxic effects.
For research use only. We do not sell to patients.
- Purity: 99.98%
- CAS No.: 256955-84-7
- Formula: C26H40N2O3
- Molecular Weight:428.61
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
In mice, Co 134444 (0.5-10 mg/kg, IP, 15 min; 1-60 mg/kg, PO; 30 min) demonstrates efficacy against pentylenetetrazol (PTZ)-induced seizures with an ED50 of 3.3 mg/kg after IP administration and 9.8 mg/kg orally. Similarly, protection against maximal electroshock (MES) is observed with an ED50 of 4.8 mg/kg IP and 20.6 mg/kg PO. In rats, Co134444 (10-40 mg/kg; PO; 30 min) protects against PTZ-induced seizures and MES-induced seizures with an ED50 of 23.6 mg/kg and 25.3 mg/kg, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 256955-84-7
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Appearance Solid
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Molecular Weight 428.61
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Formula C26H40N2O3
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Color White to off-white
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SMILES
O=C([C@@]1([H])CC[C@@]2([H])[C@]3([H])CC[C@@]4([H])C[C@](COC)(O)CC[C@]4(C)[C@@]3([H])CC[C@@]21C)CN5C=CN=C5
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Synonyms
Co 134444
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (233.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.83 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3331 mL | 11.6656 mL | 23.3312 mL | 58.3281 mL |
| 5 mM | 0.4666 mL | 2.3331 mL | 4.6662 mL | 11.6656 mL | |
| 10 mM | 0.2333 mL | 1.1666 mL | 2.3331 mL | 5.8328 mL | |
| 15 mM | 0.1555 mL | 0.7777 mL | 1.5554 mL | 3.8885 mL | |
| 20 mM | 0.1167 mL | 0.5833 mL | 1.1666 mL | 2.9164 mL | |
| 25 mM | 0.0933 mL | 0.4666 mL | 0.9332 mL | 2.3331 mL | |
| 30 mM | 0.0778 mL | 0.3889 mL | 0.7777 mL | 1.9443 mL | |
| 40 mM | 0.0583 mL | 0.2916 mL | 0.5833 mL | 1.4582 mL | |
| 50 mM | 0.0467 mL | 0.2333 mL | 0.4666 mL | 1.1666 mL | |
| 60 mM | 0.0389 mL | 0.1944 mL | 0.3889 mL | 0.9721 mL | |
| 80 mM | 0.0292 mL | 0.1458 mL | 0.2916 mL | 0.7291 mL | |
| 100 mM | 0.0233 mL | 0.1167 mL | 0.2333 mL | 0.5833 mL |
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