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Cat. No.: HY-D1022

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Biotin-16-dUTP

(Synonyms: Biotin-16-deoxyuridine-5'-triphosphate)

Biotin-16-dUTP (Biotin-16-deoxyuridine-5'-triphosphate) can be used to replace its natural counterpart dTTP by enzymatically incorporating it into DNA/cDNA. Biotin-16- dUTP can be used to produce biotinylated DNA probes in a variety of assay applications.

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CAS No.: 86303-26-6
Formule: C₃₂H₅₂N₇O₁₈P₃S
Masse moléculaire:947.78

Stockage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (263 KB)

Biotin-16-dUTP Chemical Structure

CAS No.: 86303-26-6

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- In Vitro
Chemical Information
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
Bioz

Activité biologique

Description

In Vitro

Biotin-16-dUTP trisodium can be used for in situ detection of DNA fragments by nick end labelling (TUNEL assay) for the study of apoptosis^[1].
Biotin-16-dUTP trisodium can be used for replication foci labelling in permeabilised HeLa cell nuclei and detected by fluorescent streptavidin^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 86303-26-6
Masse moléculaire 947.78
Formule C₃₂H₅₂N₇O₁₈P₃S
SMILES

O[C@H]1C[C@H](N(C=C2/C=C/CNC(CCCCCNC(CCCNC(CCCC[C@@H]3SC[C@@]4(NC(N[C@@]43[H])=O)[H])=O)=O)=O)C(NC2=O)=O)O[C@@H]1COP(O)(OP(O)(OP(O)(O)=O)=O)=O
Synonyms

Biotin-16-deoxyuridine-5'-triphosphate
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (263 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. C Charriaut-Marlangue, et al. A cautionary note on the use of the TUNEL stain to determine apoptosis. Neuroreport. 1995 Dec 29;7(1):61-4. [Content Brief]

[2]. T Krude, et al. Chromatin assembly factor 1 (CAF-1) colocalizes with replication foci in HeLa cell nuclei. Exp Cell Res. 1995 Oct;220(2):304-11. [Content Brief]

Calculators

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight Molecular Weight

Calculateur de dilution

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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