Biotin-16-dUTP trisodium
Based on 1 Customer Validation
Biotin-16- dUTP (Biotin-16-deoxyuridine-5'-triphosphate) trisodium can be used to replace its natural counterpart dTTP by enzymatically incorporating it into DNA/cDNA. Biotin-16- dUTP trisodium can be used to produce biotinylated DNA probes in a variety of assay applications.
For research use only. We do not sell to patients.
- Purity: 99%
- Formula: C32H49N7Na3O18P3S
- Molecular Weight:1013.72
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Storage:
Solution, -20°C, protect from light, 2 years
Biological Activity
Biotin-16- dUTP trisodium can be used for in situ detection of DNA fragments by nick end labelling (TUNEL assay) for the study of apoptosis[1].
Biotin-16-dUTP trisodium can be used for replication foci labelling in permeabilised HeLa cell nuclei and detected by fluorescent streptavidin[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Liquid
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Molecular Weight 1013.72
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Formula C32H49N7Na3O18P3S
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Color Colorless to light yellow
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SMILES
O=C(NC1=O)N([C@]2([H])O[C@@H]([C@H](C2)O)COP(OP(OP(O)(O[Na])=O)(O[Na])=O)(O[Na])=O)C=C1/C=C/CNC(CCCCCNC(CCCNC(CCCC[C@H]3[C@]4([H])[C@](NC(N4)=O)([H])CS3)=O)=O)=O
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Synonyms
Biotin-16-deoxyuridine-5'-triphosphate trisodium
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Solution, -20°C, protect from light, 2 years
Purity & Documentation
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Data Sheet (264 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. C Charriaut-Marlangue, et al. A cautionary note on the use of the TUNEL stain to determine apoptosis. Neuroreport. 1995 Dec 29;7(1):61-4. [Content Brief]
[2]. T Krude, et al. Chromatin assembly factor 1 (CAF-1) colocalizes with replication foci in HeLa cell nuclei. Exp Cell Res. 1995 Oct;220(2):304-11. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)