Biotin-16-dUTP
Biotin-16-dUTP (Biotin-16-deoxyuridine-5'-triphosphate) can be used to replace its natural counterpart dTTP by enzymatically incorporating it into DNA/cDNA. Biotin-16- dUTP can be used to produce biotinylated DNA probes in a variety of assay applications.
For research use only. We do not sell to patients.
- CAS No.: 86303-26-6
- Formula: C32H52N7O18P3S
- Molecular Weight:947.78
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Biotin-16-dUTP trisodium can be used for in situ detection of DNA fragments by nick end labelling (TUNEL assay) for the study of apoptosis[1].
Biotin-16-dUTP trisodium can be used for replication foci labelling in permeabilised HeLa cell nuclei and detected by fluorescent streptavidin[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 86303-26-6
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Molecular Weight 947.78
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Formula C32H52N7O18P3S
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SMILES
O[C@H]1C[C@H](N(C=C2/C=C/CNC(CCCCCNC(CCCNC(CCCC[C@@H]3SC[C@@]4(NC(N[C@@]43[H])=O)[H])=O)=O)=O)C(NC2=O)=O)O[C@@H]1COP(O)(OP(O)(OP(O)(O)=O)=O)=O
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Synonyms
Biotin-16-deoxyuridine-5'-triphosphate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. C Charriaut-Marlangue, et al. A cautionary note on the use of the TUNEL stain to determine apoptosis. Neuroreport. 1995 Dec 29;7(1):61-4. [Content Brief]
[2]. T Krude, et al. Chromatin assembly factor 1 (CAF-1) colocalizes with replication foci in HeLa cell nuclei. Exp Cell Res. 1995 Oct;220(2):304-11. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)