Brusatol
Based on 45 publication(s) in Google Scholar
Brusatol (NSC 172924) is a unique inhibitor of the Nrf2 pathway that sensitizes a broad spectrum of cancer cells to Cisplatin and other chemotherapeutic agents. Brusatol enhances the efficacy of chemotherapy by inhibiting the Nrf2-mediated defense mechanism. Brusatol can be developed into an adjuvant chemotherapeutic agent. Brusatol increases cellular apoptosis.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté: 99.94%
- CAS No.: 14907-98-3
- Formule: C26H32O11
- Masse moléculaire:520.53
-
Stockage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 1 year; -20°C, 6 months (protect from light, stored under nitrogen)
Publications Citing Use of MedChemExpress (MCE) Brusatol
More- Nat Commun. 2024 Aug 3;15(1):6587. [Abstract]
- Adv Sci (Weinh). 2026 Mar;13(16):e13310. [Abstract]
- Adv Sci (Weinh). 2024 Dec 16:e2411479. [Abstract]
- Int J Biol Sci. 2026 Apr 23;22(9):4618-4632.
- Cell Commun Signal. 2022 Oct 27;20(1):168. [Abstract]
- Phytomedicine. 2025 May 30:144:156912. [Abstract]
- Phytomedicine. 2025 Feb 12:139:156464. [Abstract]
- Phytomedicine. 2025 Apr:139:156468. [Abstract]
- Free Radic Biol Med. 2026 Jun 3:253:596-611. [Abstract]
- Free Radic Biol Med. 2020 Nov 20;160:820-836. [Abstract]
- Neoplasia. 2021 Dec;23(12):1227-1239. [Abstract]
- Biomed Pharmacother. 2024 Jan:170:116006. [Abstract]
- Redox Rep. 2023 Dec;28(1):2158526. [Abstract]
- Antioxidants (Basel). 2023 Nov 13;12(11):1999. [Abstract]
- J Agric Food Chem. 2022 Nov 16;70(45):14414-14426. [Abstract]
- Ecotoxicol Environ Saf. 2024 May 9:278:116439. [Abstract]
- Food Biosci. 2024 Oct.
- Food Funct. 2026 Jan 26;17(2):1061-1073. [Abstract]
- Chem Biol Interact. 2024 Apr 25:393:110956. [Abstract]
- Food Funct. 2023 Nov 13;14(22):10052-10068. [Abstract]
- Inflammopharmacology. 2024 Dec;32(6):3745-3759. [Abstract]
- Int J Mol Sci. 2023 May 5;24(9):8265. [Abstract]
- Front Pharmacol. 2021 Dec 14:12:775680. [Abstract]
- Int Immunopharmacol. 2026 Apr 1:174:116398. [Abstract]
- Int Immunopharmacol. 2024 Nov 6;143(Pt 3):113542. [Abstract]
- Int Immunopharmacol. 2024 Jan 5:126:111183. [Abstract]
- Eur J Pharmacol. 2020 Oct 5;884:173408. [Abstract]
- Invest Ophthalmol Vis Sci. 2020 Jun 3;61(6):51. [Abstract]
- RSC Adv. 2025 Apr 14;15(15):11675-11687. [Abstract]
- Nanomedicine. 2024;19(28):2357-2373. [Abstract]
- J Inflamm Res. 2021 Dec 10;14:6765-6782. [Abstract]
- Sci Rep. 2026 Jun 3.
- Sci Rep. 2026 Apr 3;16(1):15961.
- Sci Rep. 2024 Aug 30;14(1):20180. [Abstract]
- Cell Signal. 2023 May:105:110626. [Abstract]
- J Immunol Res. 2021 Nov 1;2021:5151168. [Abstract]
- Mol Med Rep. 2021 Nov;24(5):754. [Abstract]
- Toxicol Appl Pharmacol. 2025 Sep 22:505:117578. [Abstract]
- J Pharm Pharmacol. 2023 Apr 17;75(5):666-676. [Abstract]
- Mol Biol Rep. 2026 Jan 24;53(1):324. [Abstract]
- Inhal Toxicol. 2026 May;38(5):237-251.
- Braz J Med Biol Res. 2022 Oct 17;55:e12040. [Abstract]
- Research Square Preprint. 2024 Dec 02.
- bioRxiv. 2024 Jun 28:2024.06.24.600383. [Abstract]
- Oxid Med Cell Longev. 2021 Jun 16:2021:6696015. [Abstract]
-
Cell Proliferation/Viability Assay
-
Bio/Physico-chemical Assay
-
RT-PCR
-
Bio/Physico-chemical Assay
-
Bio/Physico-chemical Assay
Activité biologique
Nrf2[1]
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
0.067 μM
Compound: 9a
|
Antiproliferative activity against human HCT-116 cells incubated for 72 hrs by WST-1 assay
Antiproliferative activity against human HCT-116 cells incubated for 72 hrs by WST-1 assay
|
[PMID: 33289552] |
| MCF7 | IC50 |
0.083 μM
Compound: 9a
|
Cytotoxicity against human MCF-7 cells incubated for 72 hrs by MTT assay
Cytotoxicity against human MCF-7 cells incubated for 72 hrs by MTT assay
|
[PMID: 33289552] |
| MDA-MB-231 | IC50 |
0.081 μM
Compound: 9a
|
Cytotoxicity against human MDA-MB-231 cells incubated for 72 hrs by MTT assay
Cytotoxicity against human MDA-MB-231 cells incubated for 72 hrs by MTT assay
|
[PMID: 33289552] |
| MDA-MB-231 | IC50 |
29.19 nM
Compound: Brusatol
|
Inhibition of NRF2 in human MDA-MB-231 cells harboring ARE-GFP-Luc assessed as reduction in ARE-luciferase activity after 16 hrs by firefly luciferase reporter gene assay
Inhibition of NRF2 in human MDA-MB-231 cells harboring ARE-GFP-Luc assessed as reduction in ARE-luciferase activity after 16 hrs by firefly luciferase reporter gene assay
|
[PMID: 31270017] |
| PANC-1 | IC50 |
0.36 μM
Compound: 9a
|
Cytotoxicity against human Panc-1 cells incubated for 72 hrs by sulphorhodamine B assay
Cytotoxicity against human Panc-1 cells incubated for 72 hrs by sulphorhodamine B assay
|
[PMID: 33289552] |
| SW1990 | IC50 |
0.1 μM
Compound: 9a
|
Antiproliferative activity against human SW1990 incubated for 72 hrs by sulphorhodamine B assay
Antiproliferative activity against human SW1990 incubated for 72 hrs by sulphorhodamine B assay
|
[PMID: 33289552] |
Brusatol (0.05, 0.15, 0.45, 1.35, 4.05 and 12.15 μg/mL) reduces the viability of CT-26 cells in a dose-dependent manner with IC50 value of 0.27±0.01μg/mL. When Brusatol is combined with Cisplatin (CDDP) at a constant concentration ratio of 1:1, cell growth inhibition is markedly enhanced compared with single-agent treatment; the IC50 value of Brusatol and CDDP cotreatment is 0.19±0.02μg/mL[2].
Brusatol provokes a rapid and transient depletion of Nrf2 protein, through a posttranscriptional mechanism, in mouse Hepa-1c1c7 hepatoma cells. Brusatol sensitizes mammalian cells to chemical toxicity[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:The murine CT-26 CRC cell line
-
Concentration:0.05, 0.15, 0.45, 1.35, 4.05 and 12.15 μg/mL
-
Incubation Time:48 hours
-
Result:The viability of CT-26 cells was reduced in a dose-dependent manner, with IC50 value of 0.27±0.01 μg/mL.
-
Cell Line:Mouse Hepa-1c1c7 hepatoma cells
-
Concentration:1, 3, 10, 30, 100, 300, and 1000 nM
-
Incubation Time:2 hours
-
Result:Provoked the depletion of Nrf2, in a concentration-dependent manner within 2 h of exposure to cells.
Cisplatin (2 mg/kg) or Brusatol (2 mg/kg) alone does not inhibit tumor growth significantly, whereas in the combination group, tumor size is significantly reduced[1]. "
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Athymic nude mice 4-6 wk old bearing A549 xenografts[1]
-
Dosage:2 mg/kg
-
Administration:Treated i.p.; Cisplatin (2 mg/kg), Brusatol (2 mg/kg), or in combination every other day for a total of five times
-
Result:Nrf2 protein levels were significantly decreased at 24 h or 48 h postinjection.
Cisplatin or Brusatol alone did not inhibit tumor growth significantly, whereas in the combination group, tumor size was significantly reduced. "
Chemical Information
-
CAS No. 14907-98-3
-
Appearance Solid
-
Masse moléculaire 520.53
-
Formule C26H32O11
-
Color White to off-white
-
SMILES
CC([C@](C[C@@](O1)2[H])3[H])=C(O)C(C[C@]3(C)[C@]4([H])[C@]2(CO[C@@]5([C@@H](O)[C@@H]4O)C(OC)=O)[C@@]5([H])[C@@H](OC(/C=C(C)/C)=O)C1=O)=O
-
Synonyms
NSC 172924
-
Structure Classification
-
Initial Source
-
Livraison
Room temperature in continental US; may vary elsewhere.
-
Stockage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 1 year; -20°C, 6 months (protect from light, stored under nitrogen)
Publications (45)
-
Journal Impact Factor
-
Most Recent
-
Nat Commun
2024 Aug 3;15(1):6587. PMID: 39097623
Brusatol purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Aug 3;15(1):6587. [Abstract]
NIC cells were treated with the Nrf2 inhibitors, Brusatol (100 nM), ML385 (2μM)), or vehicle (DMSO). Proliferation was assessed by Incucyte after 72 h.
Brusatol purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Aug 3;15(1):6587. [Abstract]
Glucose consumption in NIC cells treated with the Nrf2 inhibitors, Brusatol (100 nM), ML385 (2μM)), or vehicle (DMSO) for 72h.
Brusatol purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Aug 3;15(1):6587. [Abstract]
QRT-PCR analysis of Glut1 gene expression in NIC cells treated with the Nrf2 inhibitors, Brusatol (100 nM), ML385 (2μM)), or vehicle (DMSO) for 72h.
Brusatol purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Aug 3;15(1):6587. [Abstract]
Basal, maximal (Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone – FCCP), ATP-synthesis coupled (Oligomycin A), and non-mitochondrial (rotenone/ antimycin A) oxygen consumption rates (OCRs) of NIC cells treated with DMSO or Nrf2 inhibitor Brusatol (100 nM).
Brusatol purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Aug 3;15(1):6587. [Abstract]
Quantification of basal OCR and extracellular acidification rates (ECAR) of NIC cells treated with the Nrf2 inhibitors, Brusatol (100 nM), ML385 (2μM)), or vehicle (DMSO) for 72h.
-
Adv Sci (Weinh)
2026 Mar;13(16):e13310. PMID: 41589654 -
Adv Sci (Weinh)
Addressing Clinical Limitations of Glutaminase Inhibitors: Novel Strategies for Osimertinib-Resistant Lung Cancer by Exploiting Glutamine Metabolic Dependency. [Abstract]2024 Dec 16:e2411479. PMID: 39680480 -
-
Cell Commun Signal
DNMT3A R882H mutation drives daunorubicin resistance in acute myeloid leukemia via regulating NRF2/NQO1 pathway. [Abstract]2022 Oct 27;20(1):168. PMID: 36303144 -
Phytomedicine
Brusatol induced ferroptosis in osteosarcoma cells by modulating the Keap1/Nrf2/SLC7A11 signaling pathway. [Abstract]2025 May 30:144:156912. PMID: 40494017 -
Phytomedicine
Brusatol inhibits malignant phenotypes and lipid metabolism of osteosarcoma cells by regulating PI3K/AKT and MAPK pathways. [Abstract]2025 Feb 12:139:156464. PMID: 39970856 -
Phytomedicine
Proteomic screening identifies brusatol targets TGFβRII to suppresses non-small cell lung cancer metastasis. [Abstract]2025 Apr:139:156468. PMID: 39978271 -
Free Radic Biol Med
Therapeutic potential of 2-hexyl-1-decanol in Cutibacterium acnes-induced skin inflammation via modulation of oxidative and inflammatory pathways. [Abstract]2026 Jun 3:253:596-611. PMID: 42242598 -
Free Radic Biol Med
BRG1 protects the heart from acute myocardial infarction by reducing oxidative damage through the activation of the NRF2/HO1 signaling pathway. [Abstract]2020 Nov 20;160:820-836. PMID: 32950688 -
Neoplasia
ABCC5 facilitates the acquired resistance of sorafenib through the inhibition of SLC7A11-induced ferroptosis in hepatocellular carcinoma. [Abstract]2021 Dec;23(12):1227-1239. PMID: 34768109 -
Biomed Pharmacother
Isoliquiritigenin inhibits apoptosis and ameliorates oxidative stress in rheumatoid arthritis chondrocytes through the Nrf2/HO-1-mediated pathway. [Abstract]2024 Jan:170:116006. PMID: 38091640 -
Redox Rep
Dexmedetomidine postconditioning attenuates myocardial ischemia/reperfusion injury by activating the Nrf2/Sirt3/SOD2 signaling pathway in the rats. [Abstract]2023 Dec;28(1):2158526. PMID: 36738240 -
Antioxidants (Basel)
Astrocytic Nrf2 Mediates the Neuroprotective and Anti-Inflammatory Effects of Nootkatone in an MPTP-Induced Parkinson's Disease Mouse Model. [Abstract]2023 Nov 13;12(11):1999. PMID: 38001852 -
J Agric Food Chem
Lycopene Alleviates Chronic Stress-Induced Liver Injury by Inhibiting Oxidative Stress-Mediated Endoplasmic Reticulum Stress Pathway Apoptosis in Rats. [Abstract]2022 Nov 16;70(45):14414-14426. PMID: 36318656 -
Ecotoxicol Environ Saf
Exposure to polystyrene nanoplastics induces hepatotoxicity involving NRF2-NLRP3 signaling pathway in mice. [Abstract]2024 May 9:278:116439. PMID: 38728945 -
-
Food Funct
Chlorogenic acid alleviates chronic restraint stress-induced liver injury potentially via Nrf2-mediated inhibition of ferroptosis and mitochondrial apoptosis. [Abstract]2026 Jan 26;17(2):1061-1073. PMID: 41524429 -
Chem Biol Interact
Aldo-keto reductase-7A2 protects against atorvastatin-induced hepatotoxicity via Nrf2 activation. [Abstract]2024 Apr 25:393:110956. PMID: 38484826 -
Food Funct
Fucoxanthin alleviated myocardial ischemia and reperfusion injury through inhibition of ferroptosis via the NRF2 signaling pathway. [Abstract]2023 Nov 13;14(22):10052-10068. PMID: 37861458 -
Inflammopharmacology
A medicine and food homology formula prevents cognitive deficits by inhibiting neuroinflammation and oxidative stress via activating AEA-Trpv1-Nrf2 pathway. [Abstract]2024 Dec;32(6):3745-3759. PMID: 39305407 -
Int J Mol Sci
Natural Compounds, Optimal Combination of Brusatol and Polydatin Promote Anti-Tumor Effect in Breast Cancer by Targeting Nrf2 Signaling Pathway. [Abstract]2023 May 5;24(9):8265. PMID: 37175972 -
Front Pharmacol
Brusatol Inhibits Proliferation and Invasion of Glioblastoma by Down-Regulating the Expression of ECM1. [Abstract]2021 Dec 14:12:775680. PMID: 34970146 -
Int Immunopharmacol
An indoloquinolinone 3a alleviates lipopolysaccharide-induced acute lung injury by modulating the NF-κB and AMPK/Nrf2 signaling pathways. [Abstract]2026 Apr 1:174:116398. PMID: 41698296 -
Int Immunopharmacol
Screening of orthopedic medicines identifies raloxifene hydrochloride as a novel ferroptosis inhibitor for spinal cord injury therapy. [Abstract]2024 Nov 6;143(Pt 3):113542. PMID: 39510030 -
Int Immunopharmacol
Knockdown of Smox protects the integrity of the blood-brain barrier through antioxidant effect and Nrf2 pathway activation in stroke. [Abstract]2024 Jan 5:126:111183. PMID: 37984250 -
Eur J Pharmacol
Artesunate inhibits atherosclerosis by upregulating vascular smooth muscle cells-derived LPL expression via the KLF2/NRF2/TCF7L2 pathway. [Abstract]2020 Oct 5;884:173408. PMID: 32739175 -
Invest Ophthalmol Vis Sci
Perillaldehyde Ameliorates Aspergillus fumigatus Keratitis by Activating the Nrf2/HO-1 Signaling Pathway and Inhibiting Dectin-1-Mediated Inflammation. [Abstract]2020 Jun 3;61(6):51. PMID: 32579678
Brusatol purchased from MedChemExpress. Usage Cited in: Invest Ophthalmol Vis Sci. 2020 Jun 3;61(6):51. [Abstract]
After pretreatment HCECs with 20 nM Brusatol (BT) for 2 hours, add 0.6 mM PAE or DMSO for 4 hours, and then stimulated with A. fumigatus for 8 hours or 24 hours. The Nrf2 and HO-1 expression declined in normal HCECs pretreated with Brusatol (BT). BT partially inhibits PAE-induced Nrf2 and HO-1 expression in infected HCECs.
Brusatol purchased from MedChemExpress. Usage Cited in: Invest Ophthalmol Vis Sci. 2020 Jun 3;61(6):51. [Abstract]
PCR and ELISA results show the mRNA and protein levels of IL-6, IL-8, and TNF-α after blocking Nrf2 activity. Brusatol (BT) pretreatment partially reverses IL-6, TNF-α, and IL-8 expression levels, which are inhibited by PAE in infected HCECs.
-
RSC Adv
Self-assembled nanoplatform-mediated co-delivery of brusatol to sensitize sorafenib for hepatocellular carcinoma treatment. [Abstract]2025 Apr 14;15(15):11675-11687. PMID: 40230634 -
Nanomedicine
Ginger-derived nanovesicles attenuate osteoarthritis progression by inhibiting oxidative stress via the Nrf2 pathway. [Abstract]2024;19(28):2357-2373. PMID: 39360651 -
J Inflamm Res
Protective Effects of Dexmedetomidine on Sepsis-Induced Vascular Leakage by Alleviating Ferroptosis via Regulating Metabolic Reprogramming. [Abstract]2021 Dec 10;14:6765-6782. PMID: 34916824 -
-
-
Sci Rep
UAMC-3203 inhibits ferroptosis and promotes functional recovery in rats with spinal cord injury. [Abstract]2024 Aug 30;14(1):20180. PMID: 39215144 -
Cell Signal
DNMT3A R882H mutation promotes acute leukemic cell survival by regulating glycolysis through the NRF2/NQO1 axis. [Abstract]2023 May:105:110626. PMID: 36758683 -
J Immunol Res
Protective Effect of Uric Acid on ox-LDL-Induced HUVECs Injury via Keap1-Nrf2-ARE Pathway. [Abstract]2021 Nov 1;2021:5151168. PMID: 34761008 -
Mol Med Rep
Atrial natriuretic peptide protects vertebral endplate chondrocytes against H2O2‑induced apoptosis and oxidative stress through activation of the Nrf2/HO‑1 signaling pathway. [Abstract]2021 Nov;24(5):754. PMID: 34476501 -
Toxicol Appl Pharmacol
7,8-Dihydroxyflavone attenuates cisplatin-induced cardiomyocyte apoptosis and mitochondrial dysfunction via the p53/Nrf2 pathway. [Abstract]2025 Sep 22:505:117578. PMID: 40992637 -
J Pharm Pharmacol
The combination of astragaloside IV and Panax notoginseng saponins attenuates cerebral ischaemia-reperfusion injury in rats through ferroptosis and inflammation inhibition via activating Nrf2. [Abstract]2023 Apr 17;75(5):666-676. PMID: 36952592 -
Mol Biol Rep
Hydrogen treatment attenuates ferroptosis and alleviates spinal cord ischemia-reperfusion injury by activating the Nrf2/HO-1 signaling pathway. [Abstract]2026 Jan 24;53(1):324. PMID: 41579273 -
-
Braz J Med Biol Res
Naringin regulates mitochondrial dynamics to protect against acetaminophen-induced hepatotoxicity by activating the AMPK/Nrf2 signaling pathway in vitro. [Abstract]2022 Oct 17;55:e12040. PMID: 36259797 -
-
bioRxiv
2024 Jun 28:2024.06.24.600383. PMID: 38979166 -
Oxid Med Cell Longev
Brusatol Inhibits Tumor Growth and Increases the Efficacy of Cabergoline against Pituitary Adenomas. [Abstract]2021 Jun 16:2021:6696015. PMID: 34221237
Solvant et solubilité
DMSO : 25 mg/mL (48.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.80 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.80 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 1 year; -20°C, 6 months (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
-
Fiche technique (283 KB)
-
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
-
Instruction de manipulation (2659 KB)
Références
[1]. Dongmei Ren, et al. Brusatol enhances the efficacy of chemotherapy by inhibiting the Nrf2-mediated defense mechanism. Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1433-8. [Content Brief]
[2]. Hai-Ming Chen, et al. Synergistic antitumor effect of brusatol combined with cisplatin on colorectal cancer cells. Int J Mol Med. 2018 Mar;41(3):1447-1454. [Content Brief]
[3]. Adedamola Olayanju, et al. Brusatol provokes a rapid and transient inhibition of Nrf2 signaling and sensitizes mammalian cells to chemical toxicity-implications for therapeutic targeting of Nrf2. Free Radic Biol Med. 2015 Jan;78:202-12. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9211 mL | 9.6056 mL | 19.2112 mL | 48.0280 mL |
| 5 mM | 0.3842 mL | 1.9211 mL | 3.8422 mL | 9.6056 mL | |
| 10 mM | 0.1921 mL | 0.9606 mL | 1.9211 mL | 4.8028 mL | |
| 15 mM | 0.1281 mL | 0.6404 mL | 1.2807 mL | 3.2019 mL | |
| 20 mM | 0.0961 mL | 0.4803 mL | 0.9606 mL | 2.4014 mL | |
| 25 mM | 0.0768 mL | 0.3842 mL | 0.7684 mL | 1.9211 mL | |
| 30 mM | 0.0640 mL | 0.3202 mL | 0.6404 mL | 1.6009 mL | |
| 40 mM | 0.0480 mL | 0.2401 mL | 0.4803 mL | 1.2007 mL |