Benzydamine N-Oxide
Based on 1 Customer Validation
Benzydamine N-Oxide is a metabolite of Benzydamine. Benzydamine N-Oxide can be used to measure flavin-containing monooxygenase activity.
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- CAS No.: 36504-71-9
- Formule: C19H23N3O2
- Masse moléculaire:325.40
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Stockage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Chemical Information
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CAS No. 36504-71-9
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Appearance Oil
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Masse moléculaire 325.40
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Formule C19H23N3O2
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Color Colorless to light yellow
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SMILES
[O-][N+](C)(CCCOC1=NN(CC2=CC=CC=C2)C3=C1C=CC=C3)C
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
DMSO : 50 mg/mL (153.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.68 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.68 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (273 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Gao C, et al. Drug metabolite synthesis by immobilized human FMO3 and whole cell catalysts. Microb Cell Fact. 2019 Aug 12;18(1):133. [Content Brief]
[2]. Yeung CK, et al. Benzydamine N-oxygenation as a measure of flavin-containing monooxygenase activity. Methods Mol Biol. 2006;320:157-62. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.0731 mL | 15.3657 mL | 30.7314 mL | 76.8285 mL |
| 5 mM | 0.6146 mL | 3.0731 mL | 6.1463 mL | 15.3657 mL | |
| 10 mM | 0.3073 mL | 1.5366 mL | 3.0731 mL | 7.6829 mL | |
| 15 mM | 0.2049 mL | 1.0244 mL | 2.0488 mL | 5.1219 mL | |
| 20 mM | 0.1537 mL | 0.7683 mL | 1.5366 mL | 3.8414 mL | |
| 25 mM | 0.1229 mL | 0.6146 mL | 1.2293 mL | 3.0731 mL | |
| 30 mM | 0.1024 mL | 0.5122 mL | 1.0244 mL | 2.5610 mL | |
| 40 mM | 0.0768 mL | 0.3841 mL | 0.7683 mL | 1.9207 mL | |
| 50 mM | 0.0615 mL | 0.3073 mL | 0.6146 mL | 1.5366 mL | |
| 60 mM | 0.0512 mL | 0.2561 mL | 0.5122 mL | 1.2805 mL | |
| 80 mM | 0.0384 mL | 0.1921 mL | 0.3841 mL | 0.9604 mL | |
| 100 mM | 0.0307 mL | 0.1537 mL | 0.3073 mL | 0.7683 mL |
Benzydamine N-Oxide Related Classifications
HY-N11425 Related Classifications
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