Janelia Fluor® 635, SE
Based on 1 Customer Validation
Janelia Fluor 635, SE (JF635, SE) is a red fluorogenic fluorescent dye containing an NHS ester that can be conjugated with primary amine groups. JF635, SE can be used for live cell imaging. Janelia Fluor? fluorescent dyes can be chemically conjugated to the specific HaloTag substrate (a chloroalkane ligand) to form a fluorescent ligand, rather than binding directly to the HaloTag protein itself. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute (Ex/Em = 635/652 nm).
For research use only. We do not sell to patients.
- Purity: 97.9%
- Formula: C33H29F2N3O6Si
- Molecular Weight:629.68
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Janelia Fluor? fluorescent dyes do not inherently possess HaloTag-binding activity; however, reactive groups such as NHS esters serve as reaction sites for covalent coupling—via chemical synthesis—to an amino-functionalized HaloTag chloroalkane ligand scaffold, thereby yielding a fluorescent HaloTag ligand.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 629.68
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Formula C33H29F2N3O6Si
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Color White to light yellow
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SMILES
O=C(C1=CC=C(C(C(C2=CC=C(C=C2[Si]3(C)C)N4CC(C4)F)=C(C3=C/5)C=CC5=[N+]6CC(C/6)F)=C1)C([O-])=O)ON7C(CCC7=O)=O
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Synonyms
JF635, SE; JF635, NHS
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : 62.96 mg/mL (99.99 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5881 mL | 7.9405 mL | 15.8811 mL | 39.7027 mL |
| 5 mM | 0.3176 mL | 1.5881 mL | 3.1762 mL | 7.9405 mL | |
| 10 mM | 0.1588 mL | 0.7941 mL | 1.5881 mL | 3.9703 mL | |
| 15 mM | 0.1059 mL | 0.5294 mL | 1.0587 mL | 2.6468 mL | |
| 20 mM | 0.0794 mL | 0.3970 mL | 0.7941 mL | 1.9851 mL | |
| 25 mM | 0.0635 mL | 0.3176 mL | 0.6352 mL | 1.5881 mL | |
| 30 mM | 0.0529 mL | 0.2647 mL | 0.5294 mL | 1.3234 mL | |
| 40 mM | 0.0397 mL | 0.1985 mL | 0.3970 mL | 0.9926 mL | |
| 50 mM | 0.0318 mL | 0.1588 mL | 0.3176 mL | 0.7941 mL | |
| 60 mM | 0.0265 mL | 0.1323 mL | 0.2647 mL | 0.6617 mL | |
| 80 mM | 0.0199 mL | 0.0993 mL | 0.1985 mL | 0.4963 mL |
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