AtPep1 TFA
Based on 1 Customer Validation
AtPep1 TFA is an endogenous inducer of innate immune responses. AtPep1 TFA can be isolated from Arabidopsis thaliana leaves. AtPep1 TFA activates the transcription of defensin (PDF1.2), the expression of PROPEP1, the synthesis of hydrogen peroxide (H2O2) near leaf veins, and the alkalization of Arabidopsis suspension cell culture medium. AtPep1 TFA induces the expression of genes encoding defensins and PR-1. AtPep1 TFA is applicable to studies related to Pythium irregulare infection and pathogen infection.
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- Pureté: 98.12%
- Formule: C104H183N39O32·xC2HF3O2
- Masse moléculaire:2491.81 (free base)
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Stockage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Activité biologique
AtPep1 (20 min) TFA induces medium alkalization in suspension-cultured Arabidopsis thaliana cells, with a half-maximal activity of approximately 0.25 nM[1].
AtPep1 (10 nM; 2 h) TFA induces the expression of PROPEP1 and PDF1.2 genes in detached leaves of Arabidopsis thaliana (Columbia ecotype). This response depends on functional jasmonic acid and ethylene pathways, and is partially dependent on NADPH oxidase activity[1].
AtPep1 (10 nM; 2 h) TFA induces vein-associated H2O2 accumulation in detached leaves of Arabidopsis thaliana (Columbia ecotype)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Masse moléculaire 2491.81 (free base)
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Formule C104H183N39O32·xC2HF3O2
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Color White to off-white
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Sequence
Ala-Thr-Lys-Val-Lys-Ala-Lys-Gln-Arg-Gly-Lys-Glu-Lys-Val-Ser-Ser-Gly-Arg-Pro-Gly-Gln-His-Asn
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Sequence Shortening
ATKVKAKQRGKEKVSSGRPGQHN
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvant et solubilité
DMSO : 25 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Pureté et documentation
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Fiche technique (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
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- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Huffaker A, et al. An endogenous peptide signal in Arabidopsis activates components of the innate immune response. Proc Natl Acad Sci U S A. 2006;103(26):10098-10103. [Content Brief]
[2]. Pearce G, et al. Structure-activity studies of AtPep1, a plant peptide signal involved in the innate immune response. Peptides. 2008;29(12):2083-2089. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)